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Low fish oil intake improves insulin sensitivity, lipid profile and muscle metabolism on insulin resistant MSG-obese rats.

Yamazaki RK, Brito GA, Coelho I, Pequitto DC, Yamaguchi AA, Borghetti G, Schiessel DL, Kryczyk M, Machado J, Rocha RE, Aikawa J, Iagher F, Naliwaiko K, Tanhoffer RA, Nunes EA, Fernandes LC - Lipids Health Dis (2011)

Bottom Line: Yet, ObFO revealed lower levels of total cholesterol (TC; 30%) and triacylglycerol (TG; 33%) compared to Ob.Low dose of fish oil supplementation (1 g/kg/day) was able to reduce TC and TG levels, in addition to improved systemic and muscle insulin sensitivity.These results lend credence to the benefits of n-3 fatty acids upon the deleterious effects of insulin resistance mechanisms.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Biological Sciences Building, Federal University of Parana, Curitiba, PR, Brazil. rkyamazaki@hotmail.com

ABSTRACT

Background: Obesity is commonly associated with diabetes, cardiovascular diseases and cancer. The purpose of this study was to determinate the effect of a lower dose of fish oil supplementation on insulin sensitivity, lipid profile, and muscle metabolism in obese rats.

Methods: Monosodium glutamate (MSG) (4 mg/g body weight) was injected in neonatal Wistar male rats. Three-month-old rats were divided in normal-weight control group (C), coconut fat-treated normal weight group (CO), fish oil-treated normal weight group (FO), obese control group (Ob), coconut fat-treated obese group (ObCO) and fish oil-treated obese group (ObFO). Obese insulin-resistant rats were supplemented with fish oil or coconut fat (1 g/kg/day) for 4 weeks. Insulin sensitivity, fasting blood biochemicals parameters, and skeletal muscle glucose metabolism were analyzed.

Results: Obese animals (Ob) presented higher Index Lee and 2.5 fold epididymal and retroperitoneal adipose tissue than C. Insulin sensitivity test (Kitt) showed that fish oil supplementation was able to maintain insulin sensitivity of obese rats (ObFO) similar to C. There were no changes in glucose and HDL-cholesterol levels amongst groups. Yet, ObFO revealed lower levels of total cholesterol (TC; 30%) and triacylglycerol (TG; 33%) compared to Ob. Finally, since exposed to insulin, ObFO skeletal muscle revealed an increase of 10% in lactate production, 38% in glycogen synthesis and 39% in oxidation of glucose compared to Ob.

Conclusions: Low dose of fish oil supplementation (1 g/kg/day) was able to reduce TC and TG levels, in addition to improved systemic and muscle insulin sensitivity. These results lend credence to the benefits of n-3 fatty acids upon the deleterious effects of insulin resistance mechanisms.

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Related in: MedlinePlus

Muscle metabolism. A. Lactate production by skeletal muscle incubated; B. Glycogen synthesis from incubated skeletal muscle; C. Glucose oxidation from skeletal muscle incubated. 100% was considered as the median of the values obtained in control group. * when compared with their respective control group; # when compared with the respective group without stimuli.
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Figure 3: Muscle metabolism. A. Lactate production by skeletal muscle incubated; B. Glycogen synthesis from incubated skeletal muscle; C. Glucose oxidation from skeletal muscle incubated. 100% was considered as the median of the values obtained in control group. * when compared with their respective control group; # when compared with the respective group without stimuli.

Mentions: Incubated skeletal muscle from FO group had 15% higher lactate production when compared to C group (p < 0.05) (Figure 3A). Coconut fat supplementation did not alter lactate production by skeletal muscle when compared to control group (p > 0.05). Insulin stimuli increased lactate production by 14% on control group and by 10% on FO group when compared with the absence of stimulus (p < 0.05). No alteration was observed in ObCO after added insulin. Interestingly, only the ObFO group plus insulin presented an increase of 10% when compared with absence of insulin (p < 0.05). In presence of insulin, C and CO did not alter lactate production when compared with their respective control groups (p > 0.05).


Low fish oil intake improves insulin sensitivity, lipid profile and muscle metabolism on insulin resistant MSG-obese rats.

Yamazaki RK, Brito GA, Coelho I, Pequitto DC, Yamaguchi AA, Borghetti G, Schiessel DL, Kryczyk M, Machado J, Rocha RE, Aikawa J, Iagher F, Naliwaiko K, Tanhoffer RA, Nunes EA, Fernandes LC - Lipids Health Dis (2011)

Muscle metabolism. A. Lactate production by skeletal muscle incubated; B. Glycogen synthesis from incubated skeletal muscle; C. Glucose oxidation from skeletal muscle incubated. 100% was considered as the median of the values obtained in control group. * when compared with their respective control group; # when compared with the respective group without stimuli.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108314&req=5

Figure 3: Muscle metabolism. A. Lactate production by skeletal muscle incubated; B. Glycogen synthesis from incubated skeletal muscle; C. Glucose oxidation from skeletal muscle incubated. 100% was considered as the median of the values obtained in control group. * when compared with their respective control group; # when compared with the respective group without stimuli.
Mentions: Incubated skeletal muscle from FO group had 15% higher lactate production when compared to C group (p < 0.05) (Figure 3A). Coconut fat supplementation did not alter lactate production by skeletal muscle when compared to control group (p > 0.05). Insulin stimuli increased lactate production by 14% on control group and by 10% on FO group when compared with the absence of stimulus (p < 0.05). No alteration was observed in ObCO after added insulin. Interestingly, only the ObFO group plus insulin presented an increase of 10% when compared with absence of insulin (p < 0.05). In presence of insulin, C and CO did not alter lactate production when compared with their respective control groups (p > 0.05).

Bottom Line: Yet, ObFO revealed lower levels of total cholesterol (TC; 30%) and triacylglycerol (TG; 33%) compared to Ob.Low dose of fish oil supplementation (1 g/kg/day) was able to reduce TC and TG levels, in addition to improved systemic and muscle insulin sensitivity.These results lend credence to the benefits of n-3 fatty acids upon the deleterious effects of insulin resistance mechanisms.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Biological Sciences Building, Federal University of Parana, Curitiba, PR, Brazil. rkyamazaki@hotmail.com

ABSTRACT

Background: Obesity is commonly associated with diabetes, cardiovascular diseases and cancer. The purpose of this study was to determinate the effect of a lower dose of fish oil supplementation on insulin sensitivity, lipid profile, and muscle metabolism in obese rats.

Methods: Monosodium glutamate (MSG) (4 mg/g body weight) was injected in neonatal Wistar male rats. Three-month-old rats were divided in normal-weight control group (C), coconut fat-treated normal weight group (CO), fish oil-treated normal weight group (FO), obese control group (Ob), coconut fat-treated obese group (ObCO) and fish oil-treated obese group (ObFO). Obese insulin-resistant rats were supplemented with fish oil or coconut fat (1 g/kg/day) for 4 weeks. Insulin sensitivity, fasting blood biochemicals parameters, and skeletal muscle glucose metabolism were analyzed.

Results: Obese animals (Ob) presented higher Index Lee and 2.5 fold epididymal and retroperitoneal adipose tissue than C. Insulin sensitivity test (Kitt) showed that fish oil supplementation was able to maintain insulin sensitivity of obese rats (ObFO) similar to C. There were no changes in glucose and HDL-cholesterol levels amongst groups. Yet, ObFO revealed lower levels of total cholesterol (TC; 30%) and triacylglycerol (TG; 33%) compared to Ob. Finally, since exposed to insulin, ObFO skeletal muscle revealed an increase of 10% in lactate production, 38% in glycogen synthesis and 39% in oxidation of glucose compared to Ob.

Conclusions: Low dose of fish oil supplementation (1 g/kg/day) was able to reduce TC and TG levels, in addition to improved systemic and muscle insulin sensitivity. These results lend credence to the benefits of n-3 fatty acids upon the deleterious effects of insulin resistance mechanisms.

Show MeSH
Related in: MedlinePlus