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Anti-inflammatory effects of pre-seasonal Th1-adjuvant vaccine to Parietaria judaica in asthmatics.

Scichilone N, Minaldi C, Santagata R, Battaglia S, Camarda G, Bellia V - J Asthma Allergy (2011)

Bottom Line: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048).In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33).Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

View Article: PubMed Central - PubMed

Affiliation: Dipartimento Biomedico di Medicina, Interna e Specialistica (Di.Bi.M.I.S.), Sezione di Pneumologia, University of Palermo, Palermo, Italy.

ABSTRACT

Background: The ultra-short course pre-seasonal allergy vaccine, containing appropriate allergoids with the adjuvant monophosphoryl lipid A (MPL), may be effective in treating allergic symptoms.

Objective: To explore the timing of the immunological responses to the pre-seasonal allergy vaccine.

Methods: Four subcutaneous injections of the active product (Pollinex Quattro) were administered to 20 Parietaria-sensitive intermittent asthmatics (M/F: 12/8; age: 48 ± 10 years; FEV(1)% predicted: 108% ± 12%) during the 6 weeks prior to the start of the pollen season. Exhaled breath condensate (EBC) was collected immediately before the first and immediately after the last injections (t(1) and t(2)), during the pollen season (t(3)) and after (t(4)) the pollen season. EBC was analyzed to determine the levels of pH and 8-isoprostane. Ten Parietaria-sensitive asthmatics served as the untreated control group at t(1) and t(2).

Results: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048). In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33). Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

Conclusion: These findings show that the ultra-short course of vaccine adjuvated with MPL acutely reduces the degree of airway inflammation, as expressed by markers of oxidative stress, and suggest that this reduction is maintained during and after the pollen season.

No MeSH data available.


Related in: MedlinePlus

Means (connected circles) for the pH levels that were assessed in the exhaled breath condensate of the study subjects. These evaluations were obtained at the time of the first (t1) and the last (t2) pre-seasonal injections, during (t3) and outside (t4) the pollen season.Notes: Symbols indicate statistically significant effects of immunotherapy in post-hoc analyses: *P = 0.049, t4 vs baseline.
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f3-jaa-4-019: Means (connected circles) for the pH levels that were assessed in the exhaled breath condensate of the study subjects. These evaluations were obtained at the time of the first (t1) and the last (t2) pre-seasonal injections, during (t3) and outside (t4) the pollen season.Notes: Symbols indicate statistically significant effects of immunotherapy in post-hoc analyses: *P = 0.049, t4 vs baseline.

Mentions: As a secondary aim, we tested whether changes in markers of airway inflammation also occurred in the study subjects during and after pollen season. This phase had only an exploratory aim, and did not include the control group. All study subjects completed the study. Five subjects who experienced mild asthma exacerbations during the study, presumably due to respiratory viral infections, had their next respective assessment postponed for 3 weeks. The EBC pH increased in comparison to the baseline values, both at t3 (7.72 ± 0.34) and t4 (7.82 ± 0.35), reaching a significantly higher level only at t4 with respect to baseline (P = 0.049) (Figure 3). Remarkably, the 8-isoprostane levels remained significantly lower than baseline during the allergy season (mean difference from baseline: −0.92 pg, P = 0.010). When the collection of EBC was performed outside the pollen season (t4), the concentrations of 8-isoprostane remained lower than the baseline values (mean difference from baseline: −0.70 pg, P = 0.048) (Figure 4). As expected, lung function assessed outside pollen season did not differ from that recorded at the beginning of the study (FEV1/FVC: 0.81 ± 0.02; FEV1% predicted: 111% ± 10%, P = 0.81).


Anti-inflammatory effects of pre-seasonal Th1-adjuvant vaccine to Parietaria judaica in asthmatics.

Scichilone N, Minaldi C, Santagata R, Battaglia S, Camarda G, Bellia V - J Asthma Allergy (2011)

Means (connected circles) for the pH levels that were assessed in the exhaled breath condensate of the study subjects. These evaluations were obtained at the time of the first (t1) and the last (t2) pre-seasonal injections, during (t3) and outside (t4) the pollen season.Notes: Symbols indicate statistically significant effects of immunotherapy in post-hoc analyses: *P = 0.049, t4 vs baseline.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108305&req=5

f3-jaa-4-019: Means (connected circles) for the pH levels that were assessed in the exhaled breath condensate of the study subjects. These evaluations were obtained at the time of the first (t1) and the last (t2) pre-seasonal injections, during (t3) and outside (t4) the pollen season.Notes: Symbols indicate statistically significant effects of immunotherapy in post-hoc analyses: *P = 0.049, t4 vs baseline.
Mentions: As a secondary aim, we tested whether changes in markers of airway inflammation also occurred in the study subjects during and after pollen season. This phase had only an exploratory aim, and did not include the control group. All study subjects completed the study. Five subjects who experienced mild asthma exacerbations during the study, presumably due to respiratory viral infections, had their next respective assessment postponed for 3 weeks. The EBC pH increased in comparison to the baseline values, both at t3 (7.72 ± 0.34) and t4 (7.82 ± 0.35), reaching a significantly higher level only at t4 with respect to baseline (P = 0.049) (Figure 3). Remarkably, the 8-isoprostane levels remained significantly lower than baseline during the allergy season (mean difference from baseline: −0.92 pg, P = 0.010). When the collection of EBC was performed outside the pollen season (t4), the concentrations of 8-isoprostane remained lower than the baseline values (mean difference from baseline: −0.70 pg, P = 0.048) (Figure 4). As expected, lung function assessed outside pollen season did not differ from that recorded at the beginning of the study (FEV1/FVC: 0.81 ± 0.02; FEV1% predicted: 111% ± 10%, P = 0.81).

Bottom Line: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048).In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33).Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

View Article: PubMed Central - PubMed

Affiliation: Dipartimento Biomedico di Medicina, Interna e Specialistica (Di.Bi.M.I.S.), Sezione di Pneumologia, University of Palermo, Palermo, Italy.

ABSTRACT

Background: The ultra-short course pre-seasonal allergy vaccine, containing appropriate allergoids with the adjuvant monophosphoryl lipid A (MPL), may be effective in treating allergic symptoms.

Objective: To explore the timing of the immunological responses to the pre-seasonal allergy vaccine.

Methods: Four subcutaneous injections of the active product (Pollinex Quattro) were administered to 20 Parietaria-sensitive intermittent asthmatics (M/F: 12/8; age: 48 ± 10 years; FEV(1)% predicted: 108% ± 12%) during the 6 weeks prior to the start of the pollen season. Exhaled breath condensate (EBC) was collected immediately before the first and immediately after the last injections (t(1) and t(2)), during the pollen season (t(3)) and after (t(4)) the pollen season. EBC was analyzed to determine the levels of pH and 8-isoprostane. Ten Parietaria-sensitive asthmatics served as the untreated control group at t(1) and t(2).

Results: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048). In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33). Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

Conclusion: These findings show that the ultra-short course of vaccine adjuvated with MPL acutely reduces the degree of airway inflammation, as expressed by markers of oxidative stress, and suggest that this reduction is maintained during and after the pollen season.

No MeSH data available.


Related in: MedlinePlus