Limits...
Anti-inflammatory effects of pre-seasonal Th1-adjuvant vaccine to Parietaria judaica in asthmatics.

Scichilone N, Minaldi C, Santagata R, Battaglia S, Camarda G, Bellia V - J Asthma Allergy (2011)

Bottom Line: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048).In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33).Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

View Article: PubMed Central - PubMed

Affiliation: Dipartimento Biomedico di Medicina, Interna e Specialistica (Di.Bi.M.I.S.), Sezione di Pneumologia, University of Palermo, Palermo, Italy.

ABSTRACT

Background: The ultra-short course pre-seasonal allergy vaccine, containing appropriate allergoids with the adjuvant monophosphoryl lipid A (MPL), may be effective in treating allergic symptoms.

Objective: To explore the timing of the immunological responses to the pre-seasonal allergy vaccine.

Methods: Four subcutaneous injections of the active product (Pollinex Quattro) were administered to 20 Parietaria-sensitive intermittent asthmatics (M/F: 12/8; age: 48 ± 10 years; FEV(1)% predicted: 108% ± 12%) during the 6 weeks prior to the start of the pollen season. Exhaled breath condensate (EBC) was collected immediately before the first and immediately after the last injections (t(1) and t(2)), during the pollen season (t(3)) and after (t(4)) the pollen season. EBC was analyzed to determine the levels of pH and 8-isoprostane. Ten Parietaria-sensitive asthmatics served as the untreated control group at t(1) and t(2).

Results: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048). In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33). Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

Conclusion: These findings show that the ultra-short course of vaccine adjuvated with MPL acutely reduces the degree of airway inflammation, as expressed by markers of oxidative stress, and suggest that this reduction is maintained during and after the pollen season.

No MeSH data available.


Related in: MedlinePlus

Individual data for the concentrations of 8-isoprostane assessed in the exhaled breath condensate of the study subjects. The blue circles represent the levels of 8-isoprostane measured before the first injection of the vaccine (t1), and the red circles represent the levels of 8-isoprostane measured immediately after the last injection of the vaccine (t2). Each individual (x axis) is expressed by a blue circle and the respective red circle.Abbreviation: pg, picograms.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3108305&req=5

f2-jaa-4-019: Individual data for the concentrations of 8-isoprostane assessed in the exhaled breath condensate of the study subjects. The blue circles represent the levels of 8-isoprostane measured before the first injection of the vaccine (t1), and the red circles represent the levels of 8-isoprostane measured immediately after the last injection of the vaccine (t2). Each individual (x axis) is expressed by a blue circle and the respective red circle.Abbreviation: pg, picograms.

Mentions: None of the volunteers reported any adverse events associated with the immunotherapy. During this phase, none of the subjects required rescue medications or anti-histamines. Treatment with pre-seasonal adjuvated vaccine did not significantly change the EBC pH (7.64 ± 0.33 at t1 and 7.67 ± 0.23 at t2; P = 0.52). In the control group the pH was 7.73 ± 0.26 and did not change at the following assessment performed 6 weeks later (7.79 ± 0.25, P = 0.33). The concentrations of 8-isoprostane in the EBC significantly dropped immediately after the ultra-short course of allergy vaccine (t2): the mean difference from baseline was −0.77 pg (P = 0.031). The baseline (t1) and post treatment (t2) values for each individual are depicted in Figure 2. In the control group, the concentrations of 8-isoprostane were not different from those of the study group at baseline (44.1 ± 1.4 pg/mL vs 44.4 ± 0.5 pg/mL, P = 0.86; study group vs controls), and remained unchanged after 6 weeks (44.6 ± 1.2 pg/mL, P = 0.58). This finding lends support to the occurrence of the early anti-inflammatory effect of immunotherapy in the study group.


Anti-inflammatory effects of pre-seasonal Th1-adjuvant vaccine to Parietaria judaica in asthmatics.

Scichilone N, Minaldi C, Santagata R, Battaglia S, Camarda G, Bellia V - J Asthma Allergy (2011)

Individual data for the concentrations of 8-isoprostane assessed in the exhaled breath condensate of the study subjects. The blue circles represent the levels of 8-isoprostane measured before the first injection of the vaccine (t1), and the red circles represent the levels of 8-isoprostane measured immediately after the last injection of the vaccine (t2). Each individual (x axis) is expressed by a blue circle and the respective red circle.Abbreviation: pg, picograms.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108305&req=5

f2-jaa-4-019: Individual data for the concentrations of 8-isoprostane assessed in the exhaled breath condensate of the study subjects. The blue circles represent the levels of 8-isoprostane measured before the first injection of the vaccine (t1), and the red circles represent the levels of 8-isoprostane measured immediately after the last injection of the vaccine (t2). Each individual (x axis) is expressed by a blue circle and the respective red circle.Abbreviation: pg, picograms.
Mentions: None of the volunteers reported any adverse events associated with the immunotherapy. During this phase, none of the subjects required rescue medications or anti-histamines. Treatment with pre-seasonal adjuvated vaccine did not significantly change the EBC pH (7.64 ± 0.33 at t1 and 7.67 ± 0.23 at t2; P = 0.52). In the control group the pH was 7.73 ± 0.26 and did not change at the following assessment performed 6 weeks later (7.79 ± 0.25, P = 0.33). The concentrations of 8-isoprostane in the EBC significantly dropped immediately after the ultra-short course of allergy vaccine (t2): the mean difference from baseline was −0.77 pg (P = 0.031). The baseline (t1) and post treatment (t2) values for each individual are depicted in Figure 2. In the control group, the concentrations of 8-isoprostane were not different from those of the study group at baseline (44.1 ± 1.4 pg/mL vs 44.4 ± 0.5 pg/mL, P = 0.86; study group vs controls), and remained unchanged after 6 weeks (44.6 ± 1.2 pg/mL, P = 0.58). This finding lends support to the occurrence of the early anti-inflammatory effect of immunotherapy in the study group.

Bottom Line: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048).In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33).Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

View Article: PubMed Central - PubMed

Affiliation: Dipartimento Biomedico di Medicina, Interna e Specialistica (Di.Bi.M.I.S.), Sezione di Pneumologia, University of Palermo, Palermo, Italy.

ABSTRACT

Background: The ultra-short course pre-seasonal allergy vaccine, containing appropriate allergoids with the adjuvant monophosphoryl lipid A (MPL), may be effective in treating allergic symptoms.

Objective: To explore the timing of the immunological responses to the pre-seasonal allergy vaccine.

Methods: Four subcutaneous injections of the active product (Pollinex Quattro) were administered to 20 Parietaria-sensitive intermittent asthmatics (M/F: 12/8; age: 48 ± 10 years; FEV(1)% predicted: 108% ± 12%) during the 6 weeks prior to the start of the pollen season. Exhaled breath condensate (EBC) was collected immediately before the first and immediately after the last injections (t(1) and t(2)), during the pollen season (t(3)) and after (t(4)) the pollen season. EBC was analyzed to determine the levels of pH and 8-isoprostane. Ten Parietaria-sensitive asthmatics served as the untreated control group at t(1) and t(2).

Results: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048). In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33). Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

Conclusion: These findings show that the ultra-short course of vaccine adjuvated with MPL acutely reduces the degree of airway inflammation, as expressed by markers of oxidative stress, and suggest that this reduction is maintained during and after the pollen season.

No MeSH data available.


Related in: MedlinePlus