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Anti-inflammatory effects of pre-seasonal Th1-adjuvant vaccine to Parietaria judaica in asthmatics.

Scichilone N, Minaldi C, Santagata R, Battaglia S, Camarda G, Bellia V - J Asthma Allergy (2011)

Bottom Line: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048).In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33).Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

View Article: PubMed Central - PubMed

Affiliation: Dipartimento Biomedico di Medicina, Interna e Specialistica (Di.Bi.M.I.S.), Sezione di Pneumologia, University of Palermo, Palermo, Italy.

ABSTRACT

Background: The ultra-short course pre-seasonal allergy vaccine, containing appropriate allergoids with the adjuvant monophosphoryl lipid A (MPL), may be effective in treating allergic symptoms.

Objective: To explore the timing of the immunological responses to the pre-seasonal allergy vaccine.

Methods: Four subcutaneous injections of the active product (Pollinex Quattro) were administered to 20 Parietaria-sensitive intermittent asthmatics (M/F: 12/8; age: 48 ± 10 years; FEV(1)% predicted: 108% ± 12%) during the 6 weeks prior to the start of the pollen season. Exhaled breath condensate (EBC) was collected immediately before the first and immediately after the last injections (t(1) and t(2)), during the pollen season (t(3)) and after (t(4)) the pollen season. EBC was analyzed to determine the levels of pH and 8-isoprostane. Ten Parietaria-sensitive asthmatics served as the untreated control group at t(1) and t(2).

Results: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048). In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33). Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

Conclusion: These findings show that the ultra-short course of vaccine adjuvated with MPL acutely reduces the degree of airway inflammation, as expressed by markers of oxidative stress, and suggest that this reduction is maintained during and after the pollen season.

No MeSH data available.


Related in: MedlinePlus

Schematic of the protocol that describes the study (see also study design). A total of 7 visit days were needed to complete the study. Phase 1 was the screening evaluation consisting of lung function assessment and skin prick testing. Phase 2 consisted of 4 visits at which the study subjects received subcutaneous injections of the active product with increasing strength. In the first week (week 0) and the last (week 6) the allergy vaccine was injected, and the biological assessment was conducted. The untreated control group also participated in this phase. Phase 3 was only conducted in the study subjects. In March and April (during pollen season) and August (after pollen season) each subject repeated the biological examination.Abbreviation: EBC, exhaled breath condensate.
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f1-jaa-4-019: Schematic of the protocol that describes the study (see also study design). A total of 7 visit days were needed to complete the study. Phase 1 was the screening evaluation consisting of lung function assessment and skin prick testing. Phase 2 consisted of 4 visits at which the study subjects received subcutaneous injections of the active product with increasing strength. In the first week (week 0) and the last (week 6) the allergy vaccine was injected, and the biological assessment was conducted. The untreated control group also participated in this phase. Phase 3 was only conducted in the study subjects. In March and April (during pollen season) and August (after pollen season) each subject repeated the biological examination.Abbreviation: EBC, exhaled breath condensate.

Mentions: The study had a longitudinal experimental design and consisted of three phases. Figure 1 describes the protocol and the time-frame of the study. A total of 7 visit days were needed to complete the study.


Anti-inflammatory effects of pre-seasonal Th1-adjuvant vaccine to Parietaria judaica in asthmatics.

Scichilone N, Minaldi C, Santagata R, Battaglia S, Camarda G, Bellia V - J Asthma Allergy (2011)

Schematic of the protocol that describes the study (see also study design). A total of 7 visit days were needed to complete the study. Phase 1 was the screening evaluation consisting of lung function assessment and skin prick testing. Phase 2 consisted of 4 visits at which the study subjects received subcutaneous injections of the active product with increasing strength. In the first week (week 0) and the last (week 6) the allergy vaccine was injected, and the biological assessment was conducted. The untreated control group also participated in this phase. Phase 3 was only conducted in the study subjects. In March and April (during pollen season) and August (after pollen season) each subject repeated the biological examination.Abbreviation: EBC, exhaled breath condensate.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108305&req=5

f1-jaa-4-019: Schematic of the protocol that describes the study (see also study design). A total of 7 visit days were needed to complete the study. Phase 1 was the screening evaluation consisting of lung function assessment and skin prick testing. Phase 2 consisted of 4 visits at which the study subjects received subcutaneous injections of the active product with increasing strength. In the first week (week 0) and the last (week 6) the allergy vaccine was injected, and the biological assessment was conducted. The untreated control group also participated in this phase. Phase 3 was only conducted in the study subjects. In March and April (during pollen season) and August (after pollen season) each subject repeated the biological examination.Abbreviation: EBC, exhaled breath condensate.
Mentions: The study had a longitudinal experimental design and consisted of three phases. Figure 1 describes the protocol and the time-frame of the study. A total of 7 visit days were needed to complete the study.

Bottom Line: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048).In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33).Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

View Article: PubMed Central - PubMed

Affiliation: Dipartimento Biomedico di Medicina, Interna e Specialistica (Di.Bi.M.I.S.), Sezione di Pneumologia, University of Palermo, Palermo, Italy.

ABSTRACT

Background: The ultra-short course pre-seasonal allergy vaccine, containing appropriate allergoids with the adjuvant monophosphoryl lipid A (MPL), may be effective in treating allergic symptoms.

Objective: To explore the timing of the immunological responses to the pre-seasonal allergy vaccine.

Methods: Four subcutaneous injections of the active product (Pollinex Quattro) were administered to 20 Parietaria-sensitive intermittent asthmatics (M/F: 12/8; age: 48 ± 10 years; FEV(1)% predicted: 108% ± 12%) during the 6 weeks prior to the start of the pollen season. Exhaled breath condensate (EBC) was collected immediately before the first and immediately after the last injections (t(1) and t(2)), during the pollen season (t(3)) and after (t(4)) the pollen season. EBC was analyzed to determine the levels of pH and 8-isoprostane. Ten Parietaria-sensitive asthmatics served as the untreated control group at t(1) and t(2).

Results: Measured pH levels were 7.64 ± 0.33 at t(1), 7.67 ± 0.23 at t(2), 7.72 ± 0.34 at t(3), and 7.82 ± 0.34 at t(4) (P = 0.049 vs baseline). 8-isoprostane levels were significantly lower than baseline at each visit (mean difference from baseline, for t(2): -0.77 pg, P = 0.031; for t(3): -0.92 pg, P = 0.010; for t(4): -0.70 pg, P = 0.048). In the control group, pH levels were 7.73 ± 0.26 at baseline and did not change after 6 weeks (7.79 ± 0.25, P = 0.33). Similarly, the concentrations of 8-isoprostane in the control group were not different from those of the study group at baseline (P = 0.86), and the levels remained unchanged after 6 weeks (P = 0.58).

Conclusion: These findings show that the ultra-short course of vaccine adjuvated with MPL acutely reduces the degree of airway inflammation, as expressed by markers of oxidative stress, and suggest that this reduction is maintained during and after the pollen season.

No MeSH data available.


Related in: MedlinePlus