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Genetic and Functional Diversity of Pseudomonas aeruginosa Lipopolysaccharide.

Lam JS, Taylor VL, Islam ST, Hao Y, Kocíncová D - Front Microbiol (2011)

Bottom Line: Most P. aeruginosa strains produce two distinct forms of O-Ag, one a homopolymer of D-rhamnose that is a common polysaccharide antigen (CPA, formerly termed A band), and the other a heteropolymer of three to five distinct (and often unique dideoxy) sugars in its repeat units, known as O-specific antigen (OSA, formerly termed B band).Compositional differences in the O units among the OSA from different strains form the basis of the International Antigenic Typing Scheme for classification via serotyping of different strains of P. aeruginosa.The focus of this review is to provide state-of-the-art knowledge on the genetic and resultant functional diversity of LPS produced by P. aeruginosa.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cellular Biology, University of Guelph Guelph, ON, Canada.

ABSTRACT
Lipopolysccharide (LPS) is an integral component of the Pseudomonas aeruginosa cell envelope, occupying the outer leaflet of the outer membrane in this Gram-negative opportunistic pathogen. It is important for bacterium-host interactions and has been shown to be a major virulence factor for this organism. Structurally, P. aeruginosa LPS is composed of three domains, namely, lipid A, core oligosaccharide, and the distal O antigen (O-Ag). Most P. aeruginosa strains produce two distinct forms of O-Ag, one a homopolymer of D-rhamnose that is a common polysaccharide antigen (CPA, formerly termed A band), and the other a heteropolymer of three to five distinct (and often unique dideoxy) sugars in its repeat units, known as O-specific antigen (OSA, formerly termed B band). Compositional differences in the O units among the OSA from different strains form the basis of the International Antigenic Typing Scheme for classification via serotyping of different strains of P. aeruginosa. The focus of this review is to provide state-of-the-art knowledge on the genetic and resultant functional diversity of LPS produced by P. aeruginosa. The underlying factors contributing to this diversity will be thoroughly discussed and presented in the context of its contributions to host-pathogen interactions and the control/prevention of infection.

No MeSH data available.


Related in: MedlinePlus

Different lipid A forms of P. aeruginosa and the proposed biosynthesis and modification pathway.
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Figure 5: Different lipid A forms of P. aeruginosa and the proposed biosynthesis and modification pathway.

Mentions: The lipid A domain of LPS is a glucosamine-based lipid that anchors the LPS in the bacterial outer membrane (OM). The structure of P. aeruginosa lipid A has been studied in great detail using both MS and NMR techniques, and it has been reviewed by several groups (Knirel et al., 2006; King et al., 2009; Moskowitz and Ernst, 2010). Similar to lipid A of other Gram-negative bacteria, P. aeruginosa lipid A is composed of a diglucosamine biphosphate backbone [4-P-β-D-GlcNII-(1 → 6)-α-D-GlcNI-(1 → P], and O- and N- linked primary and secondary fatty acids. The major structural differences of lipid A are observed in the number, the position, the nature of the linked acyl groups, and the modification of the phosphate groups (Figure 5). For example, the chain lengths of the fatty acids attached to lipid A of P. aeruginosa (C10/C12) are shorter than those of E. coli and Salmonella enterica sv. Typhimurium (C12/C14; Trent, 2004). Heterogeneity of lipid A structure has also been observed within different P. aeruginosa strains, which arises from different growth conditions and isolation sources.


Genetic and Functional Diversity of Pseudomonas aeruginosa Lipopolysaccharide.

Lam JS, Taylor VL, Islam ST, Hao Y, Kocíncová D - Front Microbiol (2011)

Different lipid A forms of P. aeruginosa and the proposed biosynthesis and modification pathway.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108286&req=5

Figure 5: Different lipid A forms of P. aeruginosa and the proposed biosynthesis and modification pathway.
Mentions: The lipid A domain of LPS is a glucosamine-based lipid that anchors the LPS in the bacterial outer membrane (OM). The structure of P. aeruginosa lipid A has been studied in great detail using both MS and NMR techniques, and it has been reviewed by several groups (Knirel et al., 2006; King et al., 2009; Moskowitz and Ernst, 2010). Similar to lipid A of other Gram-negative bacteria, P. aeruginosa lipid A is composed of a diglucosamine biphosphate backbone [4-P-β-D-GlcNII-(1 → 6)-α-D-GlcNI-(1 → P], and O- and N- linked primary and secondary fatty acids. The major structural differences of lipid A are observed in the number, the position, the nature of the linked acyl groups, and the modification of the phosphate groups (Figure 5). For example, the chain lengths of the fatty acids attached to lipid A of P. aeruginosa (C10/C12) are shorter than those of E. coli and Salmonella enterica sv. Typhimurium (C12/C14; Trent, 2004). Heterogeneity of lipid A structure has also been observed within different P. aeruginosa strains, which arises from different growth conditions and isolation sources.

Bottom Line: Most P. aeruginosa strains produce two distinct forms of O-Ag, one a homopolymer of D-rhamnose that is a common polysaccharide antigen (CPA, formerly termed A band), and the other a heteropolymer of three to five distinct (and often unique dideoxy) sugars in its repeat units, known as O-specific antigen (OSA, formerly termed B band).Compositional differences in the O units among the OSA from different strains form the basis of the International Antigenic Typing Scheme for classification via serotyping of different strains of P. aeruginosa.The focus of this review is to provide state-of-the-art knowledge on the genetic and resultant functional diversity of LPS produced by P. aeruginosa.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cellular Biology, University of Guelph Guelph, ON, Canada.

ABSTRACT
Lipopolysccharide (LPS) is an integral component of the Pseudomonas aeruginosa cell envelope, occupying the outer leaflet of the outer membrane in this Gram-negative opportunistic pathogen. It is important for bacterium-host interactions and has been shown to be a major virulence factor for this organism. Structurally, P. aeruginosa LPS is composed of three domains, namely, lipid A, core oligosaccharide, and the distal O antigen (O-Ag). Most P. aeruginosa strains produce two distinct forms of O-Ag, one a homopolymer of D-rhamnose that is a common polysaccharide antigen (CPA, formerly termed A band), and the other a heteropolymer of three to five distinct (and often unique dideoxy) sugars in its repeat units, known as O-specific antigen (OSA, formerly termed B band). Compositional differences in the O units among the OSA from different strains form the basis of the International Antigenic Typing Scheme for classification via serotyping of different strains of P. aeruginosa. The focus of this review is to provide state-of-the-art knowledge on the genetic and resultant functional diversity of LPS produced by P. aeruginosa. The underlying factors contributing to this diversity will be thoroughly discussed and presented in the context of its contributions to host-pathogen interactions and the control/prevention of infection.

No MeSH data available.


Related in: MedlinePlus