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Cereal based diets modulate some markers of oxidative stress and inflammation in lean and obese Zucker rats.

Belobrajdic DP, Lam YY, Mano M, Wittert GA, Bird AR - Nutr Metab (Lond) (2011)

Bottom Line: The potential of cereals with high antioxidant capacity for reducing oxidative stress and inflammation in obesity is unknown.These changes in circulating PAI-1 levels could not be explained by PAI-1 secretion rates from visceral or subcutaneous adipose tissue.Cereal-based diets with moderate and high antioxidant capacity elicited modest improvements in indices of oxidative stress and inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Food Futures Flagship, Adelaide, 5000, Australia. damien.belobrajdic@csiro.au.

ABSTRACT

Background: The potential of cereals with high antioxidant capacity for reducing oxidative stress and inflammation in obesity is unknown. This study investigated the impact of wheat bran, barley or a control diet (α-cellulose) on the development of oxidative stress and inflammation in lean and obese Zucker rats.

Methods: Seven wk old, lean and obese male Zucker rats (n = 8/group) were fed diets that contained wheat bran, barley or α-cellulose (control). After 3 months on these diets, systolic blood pressure was measured and plasma was analysed for glucose, insulin, lipids, oxygen radical absorbance capacity (ORAC), malondialdehyde, glutathione peroxidase and adipokine concentration (leptin, adiponectin, interleukin (IL)-1β, IL-6, TNFα, plasminogen activator inhibitor (PAI)-1, monocyte chemotactic protein (MCP)-1). Adipokine secretion rates from visceral and subcutaneous adipose tissue explants were also determined.

Results: Obese rats had higher body weight, systolic blood pressure and fasting blood lipids, glucose, insulin, leptin and IL-1β in comparison to lean rats, and these measures were not reduced by consumption of wheat bran or barley based diets. Serum ORAC tended to be higher in obese rats fed wheat bran and barley in comparison to control (p = 0.06). Obese rats had higher plasma malondialdehyde (p < 0.01) and lower plasma glutathione peroxidase concentration (p < 0.01) but these levels were not affected by diet type. PAI-1 was elevated in the plasma of obese rats, and the wheat bran diet in comparison to the control group reduced PAI-1 to levels seen in the lean rats (p < 0.05). These changes in circulating PAI-1 levels could not be explained by PAI-1 secretion rates from visceral or subcutaneous adipose tissue.

Conclusions: A 3-month dietary intervention was sufficient for Zucker obese rats to develop oxidative stress and systemic inflammation. Cereal-based diets with moderate and high antioxidant capacity elicited modest improvements in indices of oxidative stress and inflammation.

No MeSH data available.


Related in: MedlinePlus

Adipokine secretion rates from subcutaneous and visceral fat depots in lean (□) and obese (■) Zucker rats. The concentration of adipokines A) adiponectin, B) leptin, C) MCP-1, D) PAI-1 and E) IL-6 were measured in media that was incubated with adipose tissue for 24 hrs. Values are expressed as means ± SEM, n = 8. A significant difference between groups, p < 0.001 is denoted by (*).
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Figure 3: Adipokine secretion rates from subcutaneous and visceral fat depots in lean (□) and obese (■) Zucker rats. The concentration of adipokines A) adiponectin, B) leptin, C) MCP-1, D) PAI-1 and E) IL-6 were measured in media that was incubated with adipose tissue for 24 hrs. Values are expressed as means ± SEM, n = 8. A significant difference between groups, p < 0.001 is denoted by (*).

Mentions: The secretion of adipokines from the subcutaneous fat of obese rats was higher for leptin (p < 0.001) and monocyte chemoatractant protein-1 (MCP-1) (p < 0.01) and a trend to lower adiponectin (p = 0.10) in comparison to lean rats (Figure 3). The secretion of adipokines from the visceral fat of obese rats was lower for adiponectin (p < 0.05), with no change in IL-6, leptin, MCP-1 and PAI-1 (Figure 3). Diet type did not affect adipokine secretion levels from subcutaneous or visceral fat (data not shown).


Cereal based diets modulate some markers of oxidative stress and inflammation in lean and obese Zucker rats.

Belobrajdic DP, Lam YY, Mano M, Wittert GA, Bird AR - Nutr Metab (Lond) (2011)

Adipokine secretion rates from subcutaneous and visceral fat depots in lean (□) and obese (■) Zucker rats. The concentration of adipokines A) adiponectin, B) leptin, C) MCP-1, D) PAI-1 and E) IL-6 were measured in media that was incubated with adipose tissue for 24 hrs. Values are expressed as means ± SEM, n = 8. A significant difference between groups, p < 0.001 is denoted by (*).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108278&req=5

Figure 3: Adipokine secretion rates from subcutaneous and visceral fat depots in lean (□) and obese (■) Zucker rats. The concentration of adipokines A) adiponectin, B) leptin, C) MCP-1, D) PAI-1 and E) IL-6 were measured in media that was incubated with adipose tissue for 24 hrs. Values are expressed as means ± SEM, n = 8. A significant difference between groups, p < 0.001 is denoted by (*).
Mentions: The secretion of adipokines from the subcutaneous fat of obese rats was higher for leptin (p < 0.001) and monocyte chemoatractant protein-1 (MCP-1) (p < 0.01) and a trend to lower adiponectin (p = 0.10) in comparison to lean rats (Figure 3). The secretion of adipokines from the visceral fat of obese rats was lower for adiponectin (p < 0.05), with no change in IL-6, leptin, MCP-1 and PAI-1 (Figure 3). Diet type did not affect adipokine secretion levels from subcutaneous or visceral fat (data not shown).

Bottom Line: The potential of cereals with high antioxidant capacity for reducing oxidative stress and inflammation in obesity is unknown.These changes in circulating PAI-1 levels could not be explained by PAI-1 secretion rates from visceral or subcutaneous adipose tissue.Cereal-based diets with moderate and high antioxidant capacity elicited modest improvements in indices of oxidative stress and inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Food Futures Flagship, Adelaide, 5000, Australia. damien.belobrajdic@csiro.au.

ABSTRACT

Background: The potential of cereals with high antioxidant capacity for reducing oxidative stress and inflammation in obesity is unknown. This study investigated the impact of wheat bran, barley or a control diet (α-cellulose) on the development of oxidative stress and inflammation in lean and obese Zucker rats.

Methods: Seven wk old, lean and obese male Zucker rats (n = 8/group) were fed diets that contained wheat bran, barley or α-cellulose (control). After 3 months on these diets, systolic blood pressure was measured and plasma was analysed for glucose, insulin, lipids, oxygen radical absorbance capacity (ORAC), malondialdehyde, glutathione peroxidase and adipokine concentration (leptin, adiponectin, interleukin (IL)-1β, IL-6, TNFα, plasminogen activator inhibitor (PAI)-1, monocyte chemotactic protein (MCP)-1). Adipokine secretion rates from visceral and subcutaneous adipose tissue explants were also determined.

Results: Obese rats had higher body weight, systolic blood pressure and fasting blood lipids, glucose, insulin, leptin and IL-1β in comparison to lean rats, and these measures were not reduced by consumption of wheat bran or barley based diets. Serum ORAC tended to be higher in obese rats fed wheat bran and barley in comparison to control (p = 0.06). Obese rats had higher plasma malondialdehyde (p < 0.01) and lower plasma glutathione peroxidase concentration (p < 0.01) but these levels were not affected by diet type. PAI-1 was elevated in the plasma of obese rats, and the wheat bran diet in comparison to the control group reduced PAI-1 to levels seen in the lean rats (p < 0.05). These changes in circulating PAI-1 levels could not be explained by PAI-1 secretion rates from visceral or subcutaneous adipose tissue.

Conclusions: A 3-month dietary intervention was sufficient for Zucker obese rats to develop oxidative stress and systemic inflammation. Cereal-based diets with moderate and high antioxidant capacity elicited modest improvements in indices of oxidative stress and inflammation.

No MeSH data available.


Related in: MedlinePlus