Limits...
Design of a novel flow-and-shoot microbeam.

Garty G, Grad M, Jones BK, Xu Y, Xu J, Randers-Pehrson G, Attinger D, Brenner DJ - Radiat Prot Dosimetry (2010)

Bottom Line: With the proposed FAST system, RARAF expects to reach a throughput of 100,000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude.The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users.This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.

View Article: PubMed Central - PubMed

Affiliation: RARAF, Columbia University, 136 S. Broadway, Irvington, NY 10533, USA. gyg2101@columbia.edu

ABSTRACT
Presented here is a novel microbeam technology--the Flow-And-ShooT (FAST) microbeam--under development at RARAF. In this system, cells undergo controlled fluidic transport along a microfluidic channel intersecting the microbeam path. They are imaged and tracked in real-time, using a high-speed camera and dynamically targeted, using a magnetic Point and Shoot system. With the proposed FAST system, RARAF expects to reach a throughput of 100,000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude. The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users. This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.

Show MeSH
A fluorescent image of two beads flowing through a microfluidic channel. The beads are denoted by arrows, and the measured trajectories are overlaid. The dashed lines denote the boundaries of the channel.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3108275&req=5

NCQ476F3: A fluorescent image of two beads flowing through a microfluidic channel. The beads are denoted by arrows, and the measured trajectories are overlaid. The dashed lines denote the boundaries of the channel.

Mentions: In their preliminary studies, the authors used a 25 frame-per-second (fps) image-intensified camera(3) to take pictures of a fluorescent bead, then analysed the images and located its position at different times. In these experiments, the effective pixel size was 1.3 μm. Figure 3 shows an image of 5 μm diameter fluorescent beads flowing through the channel. Because of the laminar flow, the bead motion is fairly smooth and linear(11), so that the cell position at subsequent time points can be accurately predicted.Figure 3.


Design of a novel flow-and-shoot microbeam.

Garty G, Grad M, Jones BK, Xu Y, Xu J, Randers-Pehrson G, Attinger D, Brenner DJ - Radiat Prot Dosimetry (2010)

A fluorescent image of two beads flowing through a microfluidic channel. The beads are denoted by arrows, and the measured trajectories are overlaid. The dashed lines denote the boundaries of the channel.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3108275&req=5

NCQ476F3: A fluorescent image of two beads flowing through a microfluidic channel. The beads are denoted by arrows, and the measured trajectories are overlaid. The dashed lines denote the boundaries of the channel.
Mentions: In their preliminary studies, the authors used a 25 frame-per-second (fps) image-intensified camera(3) to take pictures of a fluorescent bead, then analysed the images and located its position at different times. In these experiments, the effective pixel size was 1.3 μm. Figure 3 shows an image of 5 μm diameter fluorescent beads flowing through the channel. Because of the laminar flow, the bead motion is fairly smooth and linear(11), so that the cell position at subsequent time points can be accurately predicted.Figure 3.

Bottom Line: With the proposed FAST system, RARAF expects to reach a throughput of 100,000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude.The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users.This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.

View Article: PubMed Central - PubMed

Affiliation: RARAF, Columbia University, 136 S. Broadway, Irvington, NY 10533, USA. gyg2101@columbia.edu

ABSTRACT
Presented here is a novel microbeam technology--the Flow-And-ShooT (FAST) microbeam--under development at RARAF. In this system, cells undergo controlled fluidic transport along a microfluidic channel intersecting the microbeam path. They are imaged and tracked in real-time, using a high-speed camera and dynamically targeted, using a magnetic Point and Shoot system. With the proposed FAST system, RARAF expects to reach a throughput of 100,000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude. The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users. This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.

Show MeSH