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Design of a novel flow-and-shoot microbeam.

Garty G, Grad M, Jones BK, Xu Y, Xu J, Randers-Pehrson G, Attinger D, Brenner DJ - Radiat Prot Dosimetry (2010)

Bottom Line: With the proposed FAST system, RARAF expects to reach a throughput of 100,000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude.The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users.This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.

View Article: PubMed Central - PubMed

Affiliation: RARAF, Columbia University, 136 S. Broadway, Irvington, NY 10533, USA. gyg2101@columbia.edu

ABSTRACT
Presented here is a novel microbeam technology--the Flow-And-ShooT (FAST) microbeam--under development at RARAF. In this system, cells undergo controlled fluidic transport along a microfluidic channel intersecting the microbeam path. They are imaged and tracked in real-time, using a high-speed camera and dynamically targeted, using a magnetic Point and Shoot system. With the proposed FAST system, RARAF expects to reach a throughput of 100,000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude. The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users. This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.

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Photograph of flow-through chip used for testing cell tracking.
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NCQ476F2: Photograph of flow-through chip used for testing cell tracking.

Mentions: For preliminary testing of cell flow and targeting, the authors have manufactured a PDMS microfluidic chip (Figure 2), featuring a flow-through channel, as described previously. For testing of the tracking system, the channels were sealed by plasma-bonding a standard glass cover slip. For cell irradiations, channels will be sealed to a 1-μm thick Si3N4 window, which will also serve as the beam-line vacuum window. The cross section of the channel has respective width and height of 200 and 20 μm, so that the cells, when targeted by the microbeam, flow within 20 μm of the exit window. This should be contrasted with the distance of 50–100 μm currently maintained in the PMM between the exit window and the (moving) cell dish.Figure 2.


Design of a novel flow-and-shoot microbeam.

Garty G, Grad M, Jones BK, Xu Y, Xu J, Randers-Pehrson G, Attinger D, Brenner DJ - Radiat Prot Dosimetry (2010)

Photograph of flow-through chip used for testing cell tracking.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3108275&req=5

NCQ476F2: Photograph of flow-through chip used for testing cell tracking.
Mentions: For preliminary testing of cell flow and targeting, the authors have manufactured a PDMS microfluidic chip (Figure 2), featuring a flow-through channel, as described previously. For testing of the tracking system, the channels were sealed by plasma-bonding a standard glass cover slip. For cell irradiations, channels will be sealed to a 1-μm thick Si3N4 window, which will also serve as the beam-line vacuum window. The cross section of the channel has respective width and height of 200 and 20 μm, so that the cells, when targeted by the microbeam, flow within 20 μm of the exit window. This should be contrasted with the distance of 50–100 μm currently maintained in the PMM between the exit window and the (moving) cell dish.Figure 2.

Bottom Line: With the proposed FAST system, RARAF expects to reach a throughput of 100,000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude.The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users.This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.

View Article: PubMed Central - PubMed

Affiliation: RARAF, Columbia University, 136 S. Broadway, Irvington, NY 10533, USA. gyg2101@columbia.edu

ABSTRACT
Presented here is a novel microbeam technology--the Flow-And-ShooT (FAST) microbeam--under development at RARAF. In this system, cells undergo controlled fluidic transport along a microfluidic channel intersecting the microbeam path. They are imaged and tracked in real-time, using a high-speed camera and dynamically targeted, using a magnetic Point and Shoot system. With the proposed FAST system, RARAF expects to reach a throughput of 100,000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude. The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users. This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.

Show MeSH