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Jejunal gene expression patterns correlate with severity of systemic infection in chicken.

Schokker D, Smits MA, Rebel JM - BMC Proc (2011)

Bottom Line: Not much is known about the effect of Salmonella enteritidis on changes in the developmental processes occurring in the intestine of young chicken.The functional properties of all the genes taken together present in 3 clusters exhibiting positive correlation at early time-points were compared with the functional properties of the genes displaying antagonistic correlations in 1 cluster.The genes in the latter cluster were mostly involved in cell turn-over and metabolism.

View Article: PubMed Central - HTML - PubMed

Affiliation: Wageningen UR Livestock Research, Animal Breeding and Genomics Centre, Lelystad, P,O, Box 65, 8200 AB, The Netherlands. dirkjan.schokker@wur.nl.

ABSTRACT

Background: Not much is known about the effect of Salmonella enteritidis on changes in the developmental processes occurring in the intestine of young chicken. Therefore we investigated the correlation of intestinal gene expression patterns with the severity of systemic Salmonella infections.

Methods: The number of Salmonella colony forming units (CFUs) in the liver of infected chicken were plotted against the average intestinal expression profiles of previously identified gene expression clusters. The functional properties of all the genes taken together present in 3 clusters exhibiting positive correlation at early time-points were compared with the functional properties of the genes displaying antagonistic correlations in 1 cluster. The top 5 ranking functional groups were analysed in further detail.

Results: Three clusters showed gene expression profiles which were positively correlated with the severity of systemic disease as measured by the number of Salmonella colony forming units in the liver. In these clusters, genes involved in morphological processes were predominantly present. One cluster had a profile that was negatively correlated with the severity of systemic disease, as measured by numbers of CFUs in the liver. The genes in the latter cluster were mostly involved in cell turn-over and metabolism.

Conclusions: In the developing jejunum of young chicken, both stimulatory and inhibitory gene expression mechanisms are correlated with the severity of systemic Salmonella infections.

No MeSH data available.


Related in: MedlinePlus

Correlation of average gene expression patterns of genes present in clusters F–I with Salmonella CFU per gram liver tissue in time. Cluster F shows negative correlation with the number of Colony Forming Units (CFUs) in liver from 0.33 – 4 days post infection (dpi), whereas clusters G, H and I have positive correlation in that time period.
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Figure 1: Correlation of average gene expression patterns of genes present in clusters F–I with Salmonella CFU per gram liver tissue in time. Cluster F shows negative correlation with the number of Colony Forming Units (CFUs) in liver from 0.33 – 4 days post infection (dpi), whereas clusters G, H and I have positive correlation in that time period.

Mentions: The average gene expression profiles of the genes in clusters F, G, H, and I showed irregularities at 2 days pi compared to the smooth profiles of the corresponding clusters obtained form non-infected chicken. Clusters G, H, and I showed a positive correlation with the amount of Salmonella colony forming units (CFU) in liver, whereas cluster F showed an anti-correlation (Fig. 1). These positive and negative correlations are based on the period between 8 hours pi to 4 days pi. This correlation suggests that the irregularity in gene expression patterns is most probably directly related to the systemic invasion of Salmonella from the intestinal tract.


Jejunal gene expression patterns correlate with severity of systemic infection in chicken.

Schokker D, Smits MA, Rebel JM - BMC Proc (2011)

Correlation of average gene expression patterns of genes present in clusters F–I with Salmonella CFU per gram liver tissue in time. Cluster F shows negative correlation with the number of Colony Forming Units (CFUs) in liver from 0.33 – 4 days post infection (dpi), whereas clusters G, H and I have positive correlation in that time period.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108234&req=5

Figure 1: Correlation of average gene expression patterns of genes present in clusters F–I with Salmonella CFU per gram liver tissue in time. Cluster F shows negative correlation with the number of Colony Forming Units (CFUs) in liver from 0.33 – 4 days post infection (dpi), whereas clusters G, H and I have positive correlation in that time period.
Mentions: The average gene expression profiles of the genes in clusters F, G, H, and I showed irregularities at 2 days pi compared to the smooth profiles of the corresponding clusters obtained form non-infected chicken. Clusters G, H, and I showed a positive correlation with the amount of Salmonella colony forming units (CFU) in liver, whereas cluster F showed an anti-correlation (Fig. 1). These positive and negative correlations are based on the period between 8 hours pi to 4 days pi. This correlation suggests that the irregularity in gene expression patterns is most probably directly related to the systemic invasion of Salmonella from the intestinal tract.

Bottom Line: Not much is known about the effect of Salmonella enteritidis on changes in the developmental processes occurring in the intestine of young chicken.The functional properties of all the genes taken together present in 3 clusters exhibiting positive correlation at early time-points were compared with the functional properties of the genes displaying antagonistic correlations in 1 cluster.The genes in the latter cluster were mostly involved in cell turn-over and metabolism.

View Article: PubMed Central - HTML - PubMed

Affiliation: Wageningen UR Livestock Research, Animal Breeding and Genomics Centre, Lelystad, P,O, Box 65, 8200 AB, The Netherlands. dirkjan.schokker@wur.nl.

ABSTRACT

Background: Not much is known about the effect of Salmonella enteritidis on changes in the developmental processes occurring in the intestine of young chicken. Therefore we investigated the correlation of intestinal gene expression patterns with the severity of systemic Salmonella infections.

Methods: The number of Salmonella colony forming units (CFUs) in the liver of infected chicken were plotted against the average intestinal expression profiles of previously identified gene expression clusters. The functional properties of all the genes taken together present in 3 clusters exhibiting positive correlation at early time-points were compared with the functional properties of the genes displaying antagonistic correlations in 1 cluster. The top 5 ranking functional groups were analysed in further detail.

Results: Three clusters showed gene expression profiles which were positively correlated with the severity of systemic disease as measured by the number of Salmonella colony forming units in the liver. In these clusters, genes involved in morphological processes were predominantly present. One cluster had a profile that was negatively correlated with the severity of systemic disease, as measured by numbers of CFUs in the liver. The genes in the latter cluster were mostly involved in cell turn-over and metabolism.

Conclusions: In the developing jejunum of young chicken, both stimulatory and inhibitory gene expression mechanisms are correlated with the severity of systemic Salmonella infections.

No MeSH data available.


Related in: MedlinePlus