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Selection of Mx gene genotype as genetic marker for Avian Influenza resistance in Indonesian native chicken.

Sartika T, Sulandari S, Zein MS - BMC Proc (2011)

Bottom Line: Allele frequency of Mx Gene was calculated.The productivity indicators such as age at first laying, egg weight and hen weight at first laying and egg production were also measured.Result showed that the frequency of the resistant allele (Mx+) was 65% and 60% in laying hens and in cocks, respectively, while the frequency of the susceptible allele (Mx-) was 35% and 40% in hens and cocks, resepctively.

View Article: PubMed Central - HTML - PubMed

Affiliation: Indonesian Research Institute for Animal Production (IRIAP), PO BOX 221- Bogor 16002, Indonesia. tikesartika@hotmail.com.

ABSTRACT

Background: In previous studies, the Mx Gene has been demonstrated to confer positive anti viral responses in chicken. The amino acid variation of Asn (allele A) at position 631 was specific to positive antiviral Mx/resistant, while, that of Ser (allele G) was specific to negative Mx/susceptible. This research was aimed at selecting one of the native chicken breeds which was found out to be resistant to avian influenza using molecular technique. The selected breed will then be used as the base population to improve native chicken breed in Indonesia.

Methods: Marker Assisted Selection (MAS) method was used in this research to accelerate the selection process, since the disease resistance had low heritability value. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique used to select the genotype of Mx++, Mx+- and Mx-- that corresponded to the positive antiviral activity (Mx++), or those which had positive or negative activity (Mx+-) and negative antiviral activity (Mx--). There were 200 native hens and 40 cocks used in this experiment. Allele frequency of Mx Gene was calculated. The productivity indicators such as age at first laying, egg weight and hen weight at first laying and egg production were also measured. The chicken that had Mx++ and Mx+- genotypes, were selected to produce offspring.

Results: Result showed that the frequency of the resistant allele (Mx+) was 65% and 60% in laying hens and in cocks, respectively, while the frequency of the susceptible allele (Mx-) was 35% and 40% in hens and cocks, resepctively. Age, egg weight and hen weight at first laying and egg production for susceptible genotype were slightly better than for the resistant genotype which were 172,41 VS 178,81 days; 33,94 VS 32,84 g; 1450 VS 1439 g and 54,32 VS 48,30 %, respectively.

No MeSH data available.


Related in: MedlinePlus

Egg production of native chicken
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Figure 2: Egg production of native chicken

Mentions: Hen day production curve was presented in Figure 2. Based on genotype Mx gene, hen day peak production of hen with GG genotype (64%) was slightly better than of hen with AA and AG genotype (60%). Egg production of native chicken in this study was better than in common native chicken (28%) in Indonesia [13]. Overall, the results indicated that the sensitive allele/ GG genotype tended to be better in productivity than the resistant allele/AA genotype.


Selection of Mx gene genotype as genetic marker for Avian Influenza resistance in Indonesian native chicken.

Sartika T, Sulandari S, Zein MS - BMC Proc (2011)

Egg production of native chicken
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108233&req=5

Figure 2: Egg production of native chicken
Mentions: Hen day production curve was presented in Figure 2. Based on genotype Mx gene, hen day peak production of hen with GG genotype (64%) was slightly better than of hen with AA and AG genotype (60%). Egg production of native chicken in this study was better than in common native chicken (28%) in Indonesia [13]. Overall, the results indicated that the sensitive allele/ GG genotype tended to be better in productivity than the resistant allele/AA genotype.

Bottom Line: Allele frequency of Mx Gene was calculated.The productivity indicators such as age at first laying, egg weight and hen weight at first laying and egg production were also measured.Result showed that the frequency of the resistant allele (Mx+) was 65% and 60% in laying hens and in cocks, respectively, while the frequency of the susceptible allele (Mx-) was 35% and 40% in hens and cocks, resepctively.

View Article: PubMed Central - HTML - PubMed

Affiliation: Indonesian Research Institute for Animal Production (IRIAP), PO BOX 221- Bogor 16002, Indonesia. tikesartika@hotmail.com.

ABSTRACT

Background: In previous studies, the Mx Gene has been demonstrated to confer positive anti viral responses in chicken. The amino acid variation of Asn (allele A) at position 631 was specific to positive antiviral Mx/resistant, while, that of Ser (allele G) was specific to negative Mx/susceptible. This research was aimed at selecting one of the native chicken breeds which was found out to be resistant to avian influenza using molecular technique. The selected breed will then be used as the base population to improve native chicken breed in Indonesia.

Methods: Marker Assisted Selection (MAS) method was used in this research to accelerate the selection process, since the disease resistance had low heritability value. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique used to select the genotype of Mx++, Mx+- and Mx-- that corresponded to the positive antiviral activity (Mx++), or those which had positive or negative activity (Mx+-) and negative antiviral activity (Mx--). There were 200 native hens and 40 cocks used in this experiment. Allele frequency of Mx Gene was calculated. The productivity indicators such as age at first laying, egg weight and hen weight at first laying and egg production were also measured. The chicken that had Mx++ and Mx+- genotypes, were selected to produce offspring.

Results: Result showed that the frequency of the resistant allele (Mx+) was 65% and 60% in laying hens and in cocks, respectively, while the frequency of the susceptible allele (Mx-) was 35% and 40% in hens and cocks, resepctively. Age, egg weight and hen weight at first laying and egg production for susceptible genotype were slightly better than for the resistant genotype which were 172,41 VS 178,81 days; 33,94 VS 32,84 g; 1450 VS 1439 g and 54,32 VS 48,30 %, respectively.

No MeSH data available.


Related in: MedlinePlus