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Selection of Mx gene genotype as genetic marker for Avian Influenza resistance in Indonesian native chicken.

Sartika T, Sulandari S, Zein MS - BMC Proc (2011)

Bottom Line: Allele frequency of Mx Gene was calculated.The productivity indicators such as age at first laying, egg weight and hen weight at first laying and egg production were also measured.Result showed that the frequency of the resistant allele (Mx+) was 65% and 60% in laying hens and in cocks, respectively, while the frequency of the susceptible allele (Mx-) was 35% and 40% in hens and cocks, resepctively.

View Article: PubMed Central - HTML - PubMed

Affiliation: Indonesian Research Institute for Animal Production (IRIAP), PO BOX 221- Bogor 16002, Indonesia. tikesartika@hotmail.com.

ABSTRACT

Background: In previous studies, the Mx Gene has been demonstrated to confer positive anti viral responses in chicken. The amino acid variation of Asn (allele A) at position 631 was specific to positive antiviral Mx/resistant, while, that of Ser (allele G) was specific to negative Mx/susceptible. This research was aimed at selecting one of the native chicken breeds which was found out to be resistant to avian influenza using molecular technique. The selected breed will then be used as the base population to improve native chicken breed in Indonesia.

Methods: Marker Assisted Selection (MAS) method was used in this research to accelerate the selection process, since the disease resistance had low heritability value. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique used to select the genotype of Mx++, Mx+- and Mx-- that corresponded to the positive antiviral activity (Mx++), or those which had positive or negative activity (Mx+-) and negative antiviral activity (Mx--). There were 200 native hens and 40 cocks used in this experiment. Allele frequency of Mx Gene was calculated. The productivity indicators such as age at first laying, egg weight and hen weight at first laying and egg production were also measured. The chicken that had Mx++ and Mx+- genotypes, were selected to produce offspring.

Results: Result showed that the frequency of the resistant allele (Mx+) was 65% and 60% in laying hens and in cocks, respectively, while the frequency of the susceptible allele (Mx-) was 35% and 40% in hens and cocks, resepctively. Age, egg weight and hen weight at first laying and egg production for susceptible genotype were slightly better than for the resistant genotype which were 172,41 VS 178,81 days; 33,94 VS 32,84 g; 1450 VS 1439 g and 54,32 VS 48,30 %, respectively.

No MeSH data available.


Related in: MedlinePlus

Genotyping of Mx gene by acrylamide gel AA, Genotype of Mx gene: resistant/resistant AG, Genotype of Mx gene: resistant/sensitive GG, Genotype of Mx gene: sensitive/sensitive
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Figure 1: Genotyping of Mx gene by acrylamide gel AA, Genotype of Mx gene: resistant/resistant AG, Genotype of Mx gene: resistant/sensitive GG, Genotype of Mx gene: sensitive/sensitive

Mentions: The genomic DNA of 200 hens and 40 cocks were successfully amplified. Identification of resistant and sensitive chicken Mx gene was examined by mismatch PCR-RFLP. The PCR product was cleaved with the restriction enzyme of the Rsa1 and the digested showing polymorphism bands, one band with 100 bp in length (A/A, homozygous resistant Mx allelic genes); two bands with 100 bp and 73 bp in length (A/G, heterozygous Mx allelic genes); and one band with 73 bp in length (G/G, homozygous sensitive Mx allelic gene). An example of genotyping results is presented in Figure 1, and corresponding allele frequency is presented in Table 1.


Selection of Mx gene genotype as genetic marker for Avian Influenza resistance in Indonesian native chicken.

Sartika T, Sulandari S, Zein MS - BMC Proc (2011)

Genotyping of Mx gene by acrylamide gel AA, Genotype of Mx gene: resistant/resistant AG, Genotype of Mx gene: resistant/sensitive GG, Genotype of Mx gene: sensitive/sensitive
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108233&req=5

Figure 1: Genotyping of Mx gene by acrylamide gel AA, Genotype of Mx gene: resistant/resistant AG, Genotype of Mx gene: resistant/sensitive GG, Genotype of Mx gene: sensitive/sensitive
Mentions: The genomic DNA of 200 hens and 40 cocks were successfully amplified. Identification of resistant and sensitive chicken Mx gene was examined by mismatch PCR-RFLP. The PCR product was cleaved with the restriction enzyme of the Rsa1 and the digested showing polymorphism bands, one band with 100 bp in length (A/A, homozygous resistant Mx allelic genes); two bands with 100 bp and 73 bp in length (A/G, heterozygous Mx allelic genes); and one band with 73 bp in length (G/G, homozygous sensitive Mx allelic gene). An example of genotyping results is presented in Figure 1, and corresponding allele frequency is presented in Table 1.

Bottom Line: Allele frequency of Mx Gene was calculated.The productivity indicators such as age at first laying, egg weight and hen weight at first laying and egg production were also measured.Result showed that the frequency of the resistant allele (Mx+) was 65% and 60% in laying hens and in cocks, respectively, while the frequency of the susceptible allele (Mx-) was 35% and 40% in hens and cocks, resepctively.

View Article: PubMed Central - HTML - PubMed

Affiliation: Indonesian Research Institute for Animal Production (IRIAP), PO BOX 221- Bogor 16002, Indonesia. tikesartika@hotmail.com.

ABSTRACT

Background: In previous studies, the Mx Gene has been demonstrated to confer positive anti viral responses in chicken. The amino acid variation of Asn (allele A) at position 631 was specific to positive antiviral Mx/resistant, while, that of Ser (allele G) was specific to negative Mx/susceptible. This research was aimed at selecting one of the native chicken breeds which was found out to be resistant to avian influenza using molecular technique. The selected breed will then be used as the base population to improve native chicken breed in Indonesia.

Methods: Marker Assisted Selection (MAS) method was used in this research to accelerate the selection process, since the disease resistance had low heritability value. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique used to select the genotype of Mx++, Mx+- and Mx-- that corresponded to the positive antiviral activity (Mx++), or those which had positive or negative activity (Mx+-) and negative antiviral activity (Mx--). There were 200 native hens and 40 cocks used in this experiment. Allele frequency of Mx Gene was calculated. The productivity indicators such as age at first laying, egg weight and hen weight at first laying and egg production were also measured. The chicken that had Mx++ and Mx+- genotypes, were selected to produce offspring.

Results: Result showed that the frequency of the resistant allele (Mx+) was 65% and 60% in laying hens and in cocks, respectively, while the frequency of the susceptible allele (Mx-) was 35% and 40% in hens and cocks, resepctively. Age, egg weight and hen weight at first laying and egg production for susceptible genotype were slightly better than for the resistant genotype which were 172,41 VS 178,81 days; 33,94 VS 32,84 g; 1450 VS 1439 g and 54,32 VS 48,30 %, respectively.

No MeSH data available.


Related in: MedlinePlus