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Effects of dietary plant-derived phytonutrients on the genome-wide profiles and coccidiosis resistance in the broiler chickens.

Lillehoj HS, Kim DK, Bravo DM, Lee SH - BMC Proc (2011)

Bottom Line: To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina.The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease.The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville, MD 20705, USA. Hyun.Lillehoj@ars.usda.gov.

ABSTRACT

Background: The present study was conducted to investigate the effects of dietary plant-derived phytonutrients, carvacrol, cinnamaldehyde and Capsicum oleoresin, on the translational regulation of genes associated with immunology, physiology and metabolism using high-throughput microarray analysis and in vivo disease challenge model of avian coccidiosis.

Methods: In this study, we used nutrigenomics technology to investigate the molecular and genetic mechanisms of dietary modulation of host innate immunity and metabolism by three phytonutrients. To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina. The body weight gain and fecal oocyst production were used to evaluate coccidiosis disease parameters.

Results: Analysis of global gene expression profiles of intestinal tissues from phytonutrient-fed birds indicated that Capsicum oleoresin induced the most gene changes compared to the control group where many of these genes were associated with those of metabolism and immunity. The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease. Furthermore, dietary supplementation with these phytonutrients significantly protected broiler chickens against live coccidiosis challenge infection based on body weight and parasite fecundity.

Conclusions: The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

No MeSH data available.


Related in: MedlinePlus

Body weight gains (A) and total fecal oocyst output (B) of broiler birds fed with diet containing XT6930 (5 mg/kg of carvacrol, 3 mg/kg of cinnamaldehyde, 2 mg/kg of Capsicum) post E. acervulina infection. Body weights were measured at 0 and 9 days post E. acervulina infection. Fecal oocyst outputs were collected during the 6-9 days post-infection period. *P<0.05
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Figure 4: Body weight gains (A) and total fecal oocyst output (B) of broiler birds fed with diet containing XT6930 (5 mg/kg of carvacrol, 3 mg/kg of cinnamaldehyde, 2 mg/kg of Capsicum) post E. acervulina infection. Body weights were measured at 0 and 9 days post E. acervulina infection. Fecal oocyst outputs were collected during the 6-9 days post-infection period. *P<0.05

Mentions: To further validate biological functions of carvacrol, cinnamaldehyde, and Capsicum oleoresin in a poultry infectious disease model, broiler birds fed with standard diet supplemented with the plant extract mix designated as XT6930 (5 mg/kg of carvacrol, 3 mg/kg of cinnamaldehyde, and 2 mg/kg of Capsicum oleoresin) were orally challenged with E. acervulina. Feeding with XT6930 significantly enhanced body weight gains and reduced gut lesions of E. acervulina-infected chickens (Figure 4). Furthermore, broiler chickens which were continuously fed with a standard diet supplemented with carvacrol, cinnamaldehyde and Capsicum oleoresin from hatch showed significantly reduced gut lesions and lower pro-inflammatory cytokine gene expression (data not shown) post challenge infection with E. acervulina compared to the controls fed only the standard diet.


Effects of dietary plant-derived phytonutrients on the genome-wide profiles and coccidiosis resistance in the broiler chickens.

Lillehoj HS, Kim DK, Bravo DM, Lee SH - BMC Proc (2011)

Body weight gains (A) and total fecal oocyst output (B) of broiler birds fed with diet containing XT6930 (5 mg/kg of carvacrol, 3 mg/kg of cinnamaldehyde, 2 mg/kg of Capsicum) post E. acervulina infection. Body weights were measured at 0 and 9 days post E. acervulina infection. Fecal oocyst outputs were collected during the 6-9 days post-infection period. *P<0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108230&req=5

Figure 4: Body weight gains (A) and total fecal oocyst output (B) of broiler birds fed with diet containing XT6930 (5 mg/kg of carvacrol, 3 mg/kg of cinnamaldehyde, 2 mg/kg of Capsicum) post E. acervulina infection. Body weights were measured at 0 and 9 days post E. acervulina infection. Fecal oocyst outputs were collected during the 6-9 days post-infection period. *P<0.05
Mentions: To further validate biological functions of carvacrol, cinnamaldehyde, and Capsicum oleoresin in a poultry infectious disease model, broiler birds fed with standard diet supplemented with the plant extract mix designated as XT6930 (5 mg/kg of carvacrol, 3 mg/kg of cinnamaldehyde, and 2 mg/kg of Capsicum oleoresin) were orally challenged with E. acervulina. Feeding with XT6930 significantly enhanced body weight gains and reduced gut lesions of E. acervulina-infected chickens (Figure 4). Furthermore, broiler chickens which were continuously fed with a standard diet supplemented with carvacrol, cinnamaldehyde and Capsicum oleoresin from hatch showed significantly reduced gut lesions and lower pro-inflammatory cytokine gene expression (data not shown) post challenge infection with E. acervulina compared to the controls fed only the standard diet.

Bottom Line: To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina.The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease.The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville, MD 20705, USA. Hyun.Lillehoj@ars.usda.gov.

ABSTRACT

Background: The present study was conducted to investigate the effects of dietary plant-derived phytonutrients, carvacrol, cinnamaldehyde and Capsicum oleoresin, on the translational regulation of genes associated with immunology, physiology and metabolism using high-throughput microarray analysis and in vivo disease challenge model of avian coccidiosis.

Methods: In this study, we used nutrigenomics technology to investigate the molecular and genetic mechanisms of dietary modulation of host innate immunity and metabolism by three phytonutrients. To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina. The body weight gain and fecal oocyst production were used to evaluate coccidiosis disease parameters.

Results: Analysis of global gene expression profiles of intestinal tissues from phytonutrient-fed birds indicated that Capsicum oleoresin induced the most gene changes compared to the control group where many of these genes were associated with those of metabolism and immunity. The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease. Furthermore, dietary supplementation with these phytonutrients significantly protected broiler chickens against live coccidiosis challenge infection based on body weight and parasite fecundity.

Conclusions: The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

No MeSH data available.


Related in: MedlinePlus