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Effects of dietary plant-derived phytonutrients on the genome-wide profiles and coccidiosis resistance in the broiler chickens.

Lillehoj HS, Kim DK, Bravo DM, Lee SH - BMC Proc (2011)

Bottom Line: To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina.The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease.The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville, MD 20705, USA. Hyun.Lillehoj@ars.usda.gov.

ABSTRACT

Background: The present study was conducted to investigate the effects of dietary plant-derived phytonutrients, carvacrol, cinnamaldehyde and Capsicum oleoresin, on the translational regulation of genes associated with immunology, physiology and metabolism using high-throughput microarray analysis and in vivo disease challenge model of avian coccidiosis.

Methods: In this study, we used nutrigenomics technology to investigate the molecular and genetic mechanisms of dietary modulation of host innate immunity and metabolism by three phytonutrients. To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina. The body weight gain and fecal oocyst production were used to evaluate coccidiosis disease parameters.

Results: Analysis of global gene expression profiles of intestinal tissues from phytonutrient-fed birds indicated that Capsicum oleoresin induced the most gene changes compared to the control group where many of these genes were associated with those of metabolism and immunity. The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease. Furthermore, dietary supplementation with these phytonutrients significantly protected broiler chickens against live coccidiosis challenge infection based on body weight and parasite fecundity.

Conclusions: The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

No MeSH data available.


Related in: MedlinePlus

The most reliable network of genes exhibiting >2.0-fold expression induced by cinnamaldehyde treatment. Top functions of this network are Antigen presentation, Humoral immune response and Inflammatory disease. Up- and down-regulated genes are illustrated with red and green colors, respectively. The intensity of each gene indicates the expression level of the genes.
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Figure 3: The most reliable network of genes exhibiting >2.0-fold expression induced by cinnamaldehyde treatment. Top functions of this network are Antigen presentation, Humoral immune response and Inflammatory disease. Up- and down-regulated genes are illustrated with red and green colors, respectively. The intensity of each gene indicates the expression level of the genes.

Mentions: IPA network analysis revealed that 7, 9, and 17 biologically relevant networks were associated with cinnamaldehyde, carvacrol, and Capsicum oleoresin groups, respectively. Among the networks, the three most reliable ones from each treatment were displayed in Table 1. Top functions represent the top 3 high-level functions from the Functional Analysis of a Network which represents an overview of the biological functions associated with a given network. Figure 3 describes the first network from the treatment group of cinnamaldehyde. This network includes 18 focus genes related to the functions of antigen presentation, humoral immune response, and inflammatory disease. All relationships between genes in the network are graphically represented as lines and nodes are displayed using various shapes representing the functional class of the gene product. These relationships are supported by at least one literature reference or from canonical information stored in the Ingenuity Knowledge Base.


Effects of dietary plant-derived phytonutrients on the genome-wide profiles and coccidiosis resistance in the broiler chickens.

Lillehoj HS, Kim DK, Bravo DM, Lee SH - BMC Proc (2011)

The most reliable network of genes exhibiting >2.0-fold expression induced by cinnamaldehyde treatment. Top functions of this network are Antigen presentation, Humoral immune response and Inflammatory disease. Up- and down-regulated genes are illustrated with red and green colors, respectively. The intensity of each gene indicates the expression level of the genes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108230&req=5

Figure 3: The most reliable network of genes exhibiting >2.0-fold expression induced by cinnamaldehyde treatment. Top functions of this network are Antigen presentation, Humoral immune response and Inflammatory disease. Up- and down-regulated genes are illustrated with red and green colors, respectively. The intensity of each gene indicates the expression level of the genes.
Mentions: IPA network analysis revealed that 7, 9, and 17 biologically relevant networks were associated with cinnamaldehyde, carvacrol, and Capsicum oleoresin groups, respectively. Among the networks, the three most reliable ones from each treatment were displayed in Table 1. Top functions represent the top 3 high-level functions from the Functional Analysis of a Network which represents an overview of the biological functions associated with a given network. Figure 3 describes the first network from the treatment group of cinnamaldehyde. This network includes 18 focus genes related to the functions of antigen presentation, humoral immune response, and inflammatory disease. All relationships between genes in the network are graphically represented as lines and nodes are displayed using various shapes representing the functional class of the gene product. These relationships are supported by at least one literature reference or from canonical information stored in the Ingenuity Knowledge Base.

Bottom Line: To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina.The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease.The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville, MD 20705, USA. Hyun.Lillehoj@ars.usda.gov.

ABSTRACT

Background: The present study was conducted to investigate the effects of dietary plant-derived phytonutrients, carvacrol, cinnamaldehyde and Capsicum oleoresin, on the translational regulation of genes associated with immunology, physiology and metabolism using high-throughput microarray analysis and in vivo disease challenge model of avian coccidiosis.

Methods: In this study, we used nutrigenomics technology to investigate the molecular and genetic mechanisms of dietary modulation of host innate immunity and metabolism by three phytonutrients. To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina. The body weight gain and fecal oocyst production were used to evaluate coccidiosis disease parameters.

Results: Analysis of global gene expression profiles of intestinal tissues from phytonutrient-fed birds indicated that Capsicum oleoresin induced the most gene changes compared to the control group where many of these genes were associated with those of metabolism and immunity. The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease. Furthermore, dietary supplementation with these phytonutrients significantly protected broiler chickens against live coccidiosis challenge infection based on body weight and parasite fecundity.

Conclusions: The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

No MeSH data available.


Related in: MedlinePlus