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Effects of dietary plant-derived phytonutrients on the genome-wide profiles and coccidiosis resistance in the broiler chickens.

Lillehoj HS, Kim DK, Bravo DM, Lee SH - BMC Proc (2011)

Bottom Line: To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina.The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease.The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville, MD 20705, USA. Hyun.Lillehoj@ars.usda.gov.

ABSTRACT

Background: The present study was conducted to investigate the effects of dietary plant-derived phytonutrients, carvacrol, cinnamaldehyde and Capsicum oleoresin, on the translational regulation of genes associated with immunology, physiology and metabolism using high-throughput microarray analysis and in vivo disease challenge model of avian coccidiosis.

Methods: In this study, we used nutrigenomics technology to investigate the molecular and genetic mechanisms of dietary modulation of host innate immunity and metabolism by three phytonutrients. To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina. The body weight gain and fecal oocyst production were used to evaluate coccidiosis disease parameters.

Results: Analysis of global gene expression profiles of intestinal tissues from phytonutrient-fed birds indicated that Capsicum oleoresin induced the most gene changes compared to the control group where many of these genes were associated with those of metabolism and immunity. The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease. Furthermore, dietary supplementation with these phytonutrients significantly protected broiler chickens against live coccidiosis challenge infection based on body weight and parasite fecundity.

Conclusions: The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

No MeSH data available.


Related in: MedlinePlus

Canonical Pathway analysis of differential expressed genes by the feeding of three different phytonutrients. Datasets were analyzed by the Ingenuity Pathways Analysis software. The significance is expressed as a P value that is calculated using the right-tailed Fisher’s exact test. (A) The comparison of the pathways related with (A) carbohydrate metabolism and (B) lipid metabolism. Threshold: P value=0.05.
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Figure 2: Canonical Pathway analysis of differential expressed genes by the feeding of three different phytonutrients. Datasets were analyzed by the Ingenuity Pathways Analysis software. The significance is expressed as a P value that is calculated using the right-tailed Fisher’s exact test. (A) The comparison of the pathways related with (A) carbohydrate metabolism and (B) lipid metabolism. Threshold: P value=0.05.

Mentions: Pathway and gene network analysis using IPA software showed that Capsicum oleoresin and cinnamaldehyde significantly modified the pathways related with carbohydrate metabolism (Figure 2A) such as the citrate cycle (P values: 1.95 x 10-4, and 8.91 x 10-4, respectively), and glyoxylate and dicarboxylate metabolism (P values: 2.14 x 10-2, and 1.82 x 10-2, respectively). The pathway for glycolysis/gluconeogenesis was induced by Capsicum oleoresin (P value: 4.07 x 10-2). However, in lipid metabolism, only carvacrol treatment showed statistically significant changes associated with androgen and estrogen metabolism (P value: 9.55 x 10-3) and linoleic acid metabolism (P value: 4.79 x 10-2) pathways (Figure 2B).


Effects of dietary plant-derived phytonutrients on the genome-wide profiles and coccidiosis resistance in the broiler chickens.

Lillehoj HS, Kim DK, Bravo DM, Lee SH - BMC Proc (2011)

Canonical Pathway analysis of differential expressed genes by the feeding of three different phytonutrients. Datasets were analyzed by the Ingenuity Pathways Analysis software. The significance is expressed as a P value that is calculated using the right-tailed Fisher’s exact test. (A) The comparison of the pathways related with (A) carbohydrate metabolism and (B) lipid metabolism. Threshold: P value=0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108230&req=5

Figure 2: Canonical Pathway analysis of differential expressed genes by the feeding of three different phytonutrients. Datasets were analyzed by the Ingenuity Pathways Analysis software. The significance is expressed as a P value that is calculated using the right-tailed Fisher’s exact test. (A) The comparison of the pathways related with (A) carbohydrate metabolism and (B) lipid metabolism. Threshold: P value=0.05.
Mentions: Pathway and gene network analysis using IPA software showed that Capsicum oleoresin and cinnamaldehyde significantly modified the pathways related with carbohydrate metabolism (Figure 2A) such as the citrate cycle (P values: 1.95 x 10-4, and 8.91 x 10-4, respectively), and glyoxylate and dicarboxylate metabolism (P values: 2.14 x 10-2, and 1.82 x 10-2, respectively). The pathway for glycolysis/gluconeogenesis was induced by Capsicum oleoresin (P value: 4.07 x 10-2). However, in lipid metabolism, only carvacrol treatment showed statistically significant changes associated with androgen and estrogen metabolism (P value: 9.55 x 10-3) and linoleic acid metabolism (P value: 4.79 x 10-2) pathways (Figure 2B).

Bottom Line: To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina.The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease.The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

View Article: PubMed Central - HTML - PubMed

Affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville, MD 20705, USA. Hyun.Lillehoj@ars.usda.gov.

ABSTRACT

Background: The present study was conducted to investigate the effects of dietary plant-derived phytonutrients, carvacrol, cinnamaldehyde and Capsicum oleoresin, on the translational regulation of genes associated with immunology, physiology and metabolism using high-throughput microarray analysis and in vivo disease challenge model of avian coccidiosis.

Methods: In this study, we used nutrigenomics technology to investigate the molecular and genetic mechanisms of dietary modulation of host innate immunity and metabolism by three phytonutrients. To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina. The body weight gain and fecal oocyst production were used to evaluate coccidiosis disease parameters.

Results: Analysis of global gene expression profiles of intestinal tissues from phytonutrient-fed birds indicated that Capsicum oleoresin induced the most gene changes compared to the control group where many of these genes were associated with those of metabolism and immunity. The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease. Furthermore, dietary supplementation with these phytonutrients significantly protected broiler chickens against live coccidiosis challenge infection based on body weight and parasite fecundity.

Conclusions: The results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

No MeSH data available.


Related in: MedlinePlus