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Different immune responses to three different vaccines following H6N1 low pathogenic avian influenza virus challenge in Taiwanese local chicken breeds.

Chang CS, Tixier-Boichard M, Chazara O, Lee YP, Chen CF, Chang PC, Chen JW, Bed'hom B - BMC Proc (2011)

Bottom Line: There was no effect of the H6N1 LPAIV challenge at 7 weeks of age on the subsequent responses to ND and IBD vaccine at 11 weeks of age, but, surprisingly, the H6N1 LPAIV challenge significantly affected antibody levels to IB vaccine in some breeds, since IB0 and IB14 antibody titers were lower in the challenge groups.However, there was no significant difference in IB28 antibody titers among the experimental groups.The negative effect of the H6N1 LPAIV challenge on IB vaccine response may be related to the fact that both viruses target the lung tissues, and the type of local immune response induced by LPAIV challenge may not be favourable for birds to make optimum IB-specific antibody response.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Science, National Chung-Hsing University, Taiwan, R,O,C. fattelos@gmail.com.

ABSTRACT

Background: H6N1 low pathogenic avian influenza virus (LPAIV) are frequently isolated in Taiwan and lead to significant economic losses, either directly or indirectly through association with other infectious diseases. This study investigates immune responses to three different vaccines following a H6N1 challenge in different local breeds.

Methods: Experimental animals were sampled from six local chicken breeds maintained at the National Chung-Hsing University, namely Hsin-Yi, Ju-Chi, Hua-Tung (Taiwan), Quemoy (Quemoy Island), Shek-Ki (China), Nagoya (Japan) and a specific pathogen free (SPF) White Leghorn line. A total number of 338 chickens have been distributed between a control and a challenge group, H6N1 challenge was performed at 7 weeks of age; vaccination against Newcastle Disease (ND), Infectious Bursal Disease (IBD) and Infectious Bronchitis (IB) was performed at 11 weeks. The anti-H6N1 LPAIV antibody titers were measured by ELISA at days 0, 7, 14 and 21 after challenge, and the anti-ND, anti-IBD and anti-IB antibody titers were measured by inhibition of hemagglutination test and ELISA at days 0, 14, 28 after vaccination.

Results: There was no effect of the H6N1 LPAIV challenge at 7 weeks of age on the subsequent responses to ND and IBD vaccine at 11 weeks of age, but, surprisingly, the H6N1 LPAIV challenge significantly affected antibody levels to IB vaccine in some breeds, since IB0 and IB14 antibody titers were lower in the challenge groups. However, there was no significant difference in IB28 antibody titers among the experimental groups.

Conclusions: Local breeds have different immune response to H6N1 LPAIV challenge and subsequent vaccines. Differences dealt mainly with kinetics of response and with peak values. Quemoy exhibited higher antibody levels to H6N1, ND and IBD. The negative effect of the H6N1 LPAIV challenge on IB vaccine response may be related to the fact that both viruses target the lung tissues, and the type of local immune response induced by LPAIV challenge may not be favourable for birds to make optimum IB-specific antibody response.

No MeSH data available.


Related in: MedlinePlus

Antibody titers against H6N1 LPAIV on 0, 7, 14 and 28 days post-challenge. a,b within days post-challenge with no common superscript differ significantly (P < 0.05) in Quemoy. * means all breeds have significantly different between control and challenge group.
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Figure 1: Antibody titers against H6N1 LPAIV on 0, 7, 14 and 28 days post-challenge. a,b within days post-challenge with no common superscript differ significantly (P < 0.05) in Quemoy. * means all breeds have significantly different between control and challenge group.

Mentions: There was no mortality for Quemoy, Nagoya and SPF chickens (Table 1). Ju-Chi, Hua-Tung and Shek-Ki exhibited a low mortality (one bird each), and Hsin-Yi had the highest mortality: 7 birds (22.6%, see Table 1). Most of mortality occurred between day 7 and 14 post-challenge. The analysis of variance showed a significant effect of breed on all antibody titers, no effect of sex, and a significant effect of the challenge with a significant breed with challenge interaction on antibody titers from day 7 (Fig 1). This interaction was due to differential response between breeds: Quemoy had the highest antibody titer on day 7 and 14 post-challenge, Hua-Tung showed the highest antibody titer on day 21 post-challenge, Nagoya and SPF showed lower antibody titer on day 14 and 21 post-challenge. Quemoy was the only breed to exhibit a significant antibody titer at day 7 post-challenge. The anti-AI antibody titers were significantly higher for all breeds at days 14 and 21 post-challenge.


Different immune responses to three different vaccines following H6N1 low pathogenic avian influenza virus challenge in Taiwanese local chicken breeds.

Chang CS, Tixier-Boichard M, Chazara O, Lee YP, Chen CF, Chang PC, Chen JW, Bed'hom B - BMC Proc (2011)

Antibody titers against H6N1 LPAIV on 0, 7, 14 and 28 days post-challenge. a,b within days post-challenge with no common superscript differ significantly (P < 0.05) in Quemoy. * means all breeds have significantly different between control and challenge group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108229&req=5

Figure 1: Antibody titers against H6N1 LPAIV on 0, 7, 14 and 28 days post-challenge. a,b within days post-challenge with no common superscript differ significantly (P < 0.05) in Quemoy. * means all breeds have significantly different between control and challenge group.
Mentions: There was no mortality for Quemoy, Nagoya and SPF chickens (Table 1). Ju-Chi, Hua-Tung and Shek-Ki exhibited a low mortality (one bird each), and Hsin-Yi had the highest mortality: 7 birds (22.6%, see Table 1). Most of mortality occurred between day 7 and 14 post-challenge. The analysis of variance showed a significant effect of breed on all antibody titers, no effect of sex, and a significant effect of the challenge with a significant breed with challenge interaction on antibody titers from day 7 (Fig 1). This interaction was due to differential response between breeds: Quemoy had the highest antibody titer on day 7 and 14 post-challenge, Hua-Tung showed the highest antibody titer on day 21 post-challenge, Nagoya and SPF showed lower antibody titer on day 14 and 21 post-challenge. Quemoy was the only breed to exhibit a significant antibody titer at day 7 post-challenge. The anti-AI antibody titers were significantly higher for all breeds at days 14 and 21 post-challenge.

Bottom Line: There was no effect of the H6N1 LPAIV challenge at 7 weeks of age on the subsequent responses to ND and IBD vaccine at 11 weeks of age, but, surprisingly, the H6N1 LPAIV challenge significantly affected antibody levels to IB vaccine in some breeds, since IB0 and IB14 antibody titers were lower in the challenge groups.However, there was no significant difference in IB28 antibody titers among the experimental groups.The negative effect of the H6N1 LPAIV challenge on IB vaccine response may be related to the fact that both viruses target the lung tissues, and the type of local immune response induced by LPAIV challenge may not be favourable for birds to make optimum IB-specific antibody response.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Science, National Chung-Hsing University, Taiwan, R,O,C. fattelos@gmail.com.

ABSTRACT

Background: H6N1 low pathogenic avian influenza virus (LPAIV) are frequently isolated in Taiwan and lead to significant economic losses, either directly or indirectly through association with other infectious diseases. This study investigates immune responses to three different vaccines following a H6N1 challenge in different local breeds.

Methods: Experimental animals were sampled from six local chicken breeds maintained at the National Chung-Hsing University, namely Hsin-Yi, Ju-Chi, Hua-Tung (Taiwan), Quemoy (Quemoy Island), Shek-Ki (China), Nagoya (Japan) and a specific pathogen free (SPF) White Leghorn line. A total number of 338 chickens have been distributed between a control and a challenge group, H6N1 challenge was performed at 7 weeks of age; vaccination against Newcastle Disease (ND), Infectious Bursal Disease (IBD) and Infectious Bronchitis (IB) was performed at 11 weeks. The anti-H6N1 LPAIV antibody titers were measured by ELISA at days 0, 7, 14 and 21 after challenge, and the anti-ND, anti-IBD and anti-IB antibody titers were measured by inhibition of hemagglutination test and ELISA at days 0, 14, 28 after vaccination.

Results: There was no effect of the H6N1 LPAIV challenge at 7 weeks of age on the subsequent responses to ND and IBD vaccine at 11 weeks of age, but, surprisingly, the H6N1 LPAIV challenge significantly affected antibody levels to IB vaccine in some breeds, since IB0 and IB14 antibody titers were lower in the challenge groups. However, there was no significant difference in IB28 antibody titers among the experimental groups.

Conclusions: Local breeds have different immune response to H6N1 LPAIV challenge and subsequent vaccines. Differences dealt mainly with kinetics of response and with peak values. Quemoy exhibited higher antibody levels to H6N1, ND and IBD. The negative effect of the H6N1 LPAIV challenge on IB vaccine response may be related to the fact that both viruses target the lung tissues, and the type of local immune response induced by LPAIV challenge may not be favourable for birds to make optimum IB-specific antibody response.

No MeSH data available.


Related in: MedlinePlus