Limits...
Deciphering the genetic control of innate and adaptive immune responses in pig: a combined genetic and genomic study.

Flori L, Gao Y, Oswald IP, Lefevre F, Bouffaud M, Mercat MJ, Bidanel JP, Rogel-Gaillard C - BMC Proc (2011)

Bottom Line: Improving animal robustness and resistance to pathogens by adding health criteria in selection schemes is one of the challenging objectives of the next decade.Across traits, heritability estimates reached 0.4 on average (se=0.1) and 42 of the 54 measured parameters showed moderate to high heritabilities (≥0.2), confirming that many parameters are under genetic control and could be included in selection protocols.Functional analyses revealed that the blood transcriptome is informative for part of the immunity traits and should provide relevant phenotypic information to better characterize some immunity traits.

View Article: PubMed Central - HTML - PubMed

Affiliation: INRA, UMR de Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France. claire.rogel-gaillard@jouy.inra.fr.

ABSTRACT
Improving animal robustness and resistance to pathogens by adding health criteria in selection schemes is one of the challenging objectives of the next decade. In order to better understand the genetic control of immunity in French Large White pigs, we have launched a program combining genetic and genomic studies not focussing on any particular pathogen. Animals recorded for production traits were scored for a wide range of immunity parameters three weeks after vaccination against Mycoplasma hyopneumoniae: i) total white blood cells and lymphocyte counts and proportions of various leucocyte subsets including cells harbouring IgM, γδTCR, CD4/CD8, CD16/CD2 and CD16/CD172a/MHCII, ii) innate immune response parameters (phagocytosis and in vitro production of IL1B, IL6, IL8, TNF, IL12 and IFNαafter blood stimulation), iii) adaptive immune response parameters (lymphocyte proliferation, in vitro production of IL2, IL4, IL10 and IFNγ after blood stimulation, total IgG, IgA, IgM and specific IgG levels) and iv) two acute phase proteins (C-reactive protein and haploglobin). Across traits, heritability estimates reached 0.4 on average (se=0.1) and 42 of the 54 measured parameters showed moderate to high heritabilities (≥0.2), confirming that many parameters are under genetic control and could be included in selection protocols. Functional analyses revealed that the blood transcriptome is informative for part of the immunity traits and should provide relevant phenotypic information to better characterize some immunity traits.

No MeSH data available.


Related in: MedlinePlus

Heritabilities of IR parameters. Heritability estimations equal to or higher than 0.2 are shown with their associated 95CI for each parameter.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3108228&req=5

Figure 1: Heritabilities of IR parameters. Heritability estimations equal to or higher than 0.2 are shown with their associated 95CI for each parameter.

Mentions: Genetic analyses showed that 42 parameters present moderate to high heritabilities (h2≥0.2; Figure 1). Interestingly, significant heritability estimates were obtained for parameters retrieved from various assays provided by cell counting or measured from serum or in vitro stimulated cells, suggesting that the range of heritable parameters is wide and covers various immunity-related responses. Our overall results are in agreement with previous heritability calculations [1,3,5,6] and identification of QTLs for various traits including cell counting [9,16], antibody response [17,18] and more recently serum levels of IL10 and IFNγ cytokines after a challenge by the classical swine fever vaccine [10]. It is well known that IR is highly dependent on environment. However, the growing data set on significant heritability levels of many immunity-related parameters together with the detection of QTLs strongly suggest that a large number of immune traits at various positions in the complex scheme of IR is under a consistent genetic control.


Deciphering the genetic control of innate and adaptive immune responses in pig: a combined genetic and genomic study.

Flori L, Gao Y, Oswald IP, Lefevre F, Bouffaud M, Mercat MJ, Bidanel JP, Rogel-Gaillard C - BMC Proc (2011)

Heritabilities of IR parameters. Heritability estimations equal to or higher than 0.2 are shown with their associated 95CI for each parameter.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108228&req=5

Figure 1: Heritabilities of IR parameters. Heritability estimations equal to or higher than 0.2 are shown with their associated 95CI for each parameter.
Mentions: Genetic analyses showed that 42 parameters present moderate to high heritabilities (h2≥0.2; Figure 1). Interestingly, significant heritability estimates were obtained for parameters retrieved from various assays provided by cell counting or measured from serum or in vitro stimulated cells, suggesting that the range of heritable parameters is wide and covers various immunity-related responses. Our overall results are in agreement with previous heritability calculations [1,3,5,6] and identification of QTLs for various traits including cell counting [9,16], antibody response [17,18] and more recently serum levels of IL10 and IFNγ cytokines after a challenge by the classical swine fever vaccine [10]. It is well known that IR is highly dependent on environment. However, the growing data set on significant heritability levels of many immunity-related parameters together with the detection of QTLs strongly suggest that a large number of immune traits at various positions in the complex scheme of IR is under a consistent genetic control.

Bottom Line: Improving animal robustness and resistance to pathogens by adding health criteria in selection schemes is one of the challenging objectives of the next decade.Across traits, heritability estimates reached 0.4 on average (se=0.1) and 42 of the 54 measured parameters showed moderate to high heritabilities (≥0.2), confirming that many parameters are under genetic control and could be included in selection protocols.Functional analyses revealed that the blood transcriptome is informative for part of the immunity traits and should provide relevant phenotypic information to better characterize some immunity traits.

View Article: PubMed Central - HTML - PubMed

Affiliation: INRA, UMR de Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France. claire.rogel-gaillard@jouy.inra.fr.

ABSTRACT
Improving animal robustness and resistance to pathogens by adding health criteria in selection schemes is one of the challenging objectives of the next decade. In order to better understand the genetic control of immunity in French Large White pigs, we have launched a program combining genetic and genomic studies not focussing on any particular pathogen. Animals recorded for production traits were scored for a wide range of immunity parameters three weeks after vaccination against Mycoplasma hyopneumoniae: i) total white blood cells and lymphocyte counts and proportions of various leucocyte subsets including cells harbouring IgM, γδTCR, CD4/CD8, CD16/CD2 and CD16/CD172a/MHCII, ii) innate immune response parameters (phagocytosis and in vitro production of IL1B, IL6, IL8, TNF, IL12 and IFNαafter blood stimulation), iii) adaptive immune response parameters (lymphocyte proliferation, in vitro production of IL2, IL4, IL10 and IFNγ after blood stimulation, total IgG, IgA, IgM and specific IgG levels) and iv) two acute phase proteins (C-reactive protein and haploglobin). Across traits, heritability estimates reached 0.4 on average (se=0.1) and 42 of the 54 measured parameters showed moderate to high heritabilities (≥0.2), confirming that many parameters are under genetic control and could be included in selection protocols. Functional analyses revealed that the blood transcriptome is informative for part of the immunity traits and should provide relevant phenotypic information to better characterize some immunity traits.

No MeSH data available.


Related in: MedlinePlus