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Correlation in chicken between the marker LEI0258 alleles and Major Histocompatibility Complex sequences.

Chazara O, Juul-Madsen HR, Chang CS, Tixier-Boichard M, Bed'hom B - BMC Proc (2011)

Bottom Line: Here we describe to which extent the LEI0258 alleles are associated with alleles of classical class I genes and non-classical class II genes, in reference animals as well as local breeds with unknown MHC haplotypes.Overall we found that the LEI0258 marker genotypes gave good indications of the MHC haplotypes, and a very good predictions (>0.95) of the heterozygosity of an animal at the MHC locus.Our results show that the LEI0258 alleles are strongly associated with haplotypes of classical class I genes and non-classical class II genes, unravelling the reasons why this marker is becoming the reference marker for MHC genotyping in chickens.

View Article: PubMed Central - HTML - PubMed

Affiliation: UMR 1313 Génétique Animale et Biologie Intégrative, INRA/AgroParisTech, 78352 Jouy en Josas, France. olympe.chazara@jouy.inra.fr.

ABSTRACT

Background: The LEI0258 marker is located within the B region of the chicken Major Histocompatibility Complex (MHC), and is surprisingly well associated with serology. Therefore, the correlation between the LEI0258 alleles and the MHC class I and the class II alleles at the level of sequences is worth investigating in chickens. Here we describe to which extent the LEI0258 alleles are associated with alleles of classical class I genes and non-classical class II genes, in reference animals as well as local breeds with unknown MHC haplotypes.

Methods: For the class I region, in an exploratory project, we studied 10 animals from 3 breeds: Rhode Island Red, White Leghorn and Fayoumi chickens, by cloning and sequencing B-F1 and B-F2 cDNA from exon 1 to 3'UTR. For the class II region, we reconstructed haplotypes of the 8.8 kb genomic region encompassing three non-classical class II genes: B-DMA, B-DMB1 and B-DMB2, for 146 animals from more than 50 breeds including wild species of jungle fowls.

Results: Overall we found that the LEI0258 marker genotypes gave good indications of the MHC haplotypes, and a very good predictions (>0.95) of the heterozygosity of an animal at the MHC locus.

Conclusions: Our results show that the LEI0258 alleles are strongly associated with haplotypes of classical class I genes and non-classical class II genes, unravelling the reasons why this marker is becoming the reference marker for MHC genotyping in chickens.

No MeSH data available.


Core chicken MHC B region showing gene content and positions of the primers used for PCR
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Figure 1: Core chicken MHC B region showing gene content and positions of the primers used for PCR

Mentions: The chicken MHC B class I region comprises two genes, encoding class Iα chains: B-F1 (B-FI or B-F minor) and B-F2 (B-FIV or B-F major). The B-F2 gene is described as the major expressed locus. In addition, the chicken MHC B class II region comprises three genes encoding non-classical DM chains, one α chain and two β chains: respectively B-DMA, B-DMB1 and B-DMB2 (Figure 1). At the border of the class II region, one atypical variable number of tandem repeat (VNTR) marker has been reported: the LEI0258 marker, which is highly polymorphic, with 26 allele sizes already reported [2].


Correlation in chicken between the marker LEI0258 alleles and Major Histocompatibility Complex sequences.

Chazara O, Juul-Madsen HR, Chang CS, Tixier-Boichard M, Bed'hom B - BMC Proc (2011)

Core chicken MHC B region showing gene content and positions of the primers used for PCR
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108224&req=5

Figure 1: Core chicken MHC B region showing gene content and positions of the primers used for PCR
Mentions: The chicken MHC B class I region comprises two genes, encoding class Iα chains: B-F1 (B-FI or B-F minor) and B-F2 (B-FIV or B-F major). The B-F2 gene is described as the major expressed locus. In addition, the chicken MHC B class II region comprises three genes encoding non-classical DM chains, one α chain and two β chains: respectively B-DMA, B-DMB1 and B-DMB2 (Figure 1). At the border of the class II region, one atypical variable number of tandem repeat (VNTR) marker has been reported: the LEI0258 marker, which is highly polymorphic, with 26 allele sizes already reported [2].

Bottom Line: Here we describe to which extent the LEI0258 alleles are associated with alleles of classical class I genes and non-classical class II genes, in reference animals as well as local breeds with unknown MHC haplotypes.Overall we found that the LEI0258 marker genotypes gave good indications of the MHC haplotypes, and a very good predictions (>0.95) of the heterozygosity of an animal at the MHC locus.Our results show that the LEI0258 alleles are strongly associated with haplotypes of classical class I genes and non-classical class II genes, unravelling the reasons why this marker is becoming the reference marker for MHC genotyping in chickens.

View Article: PubMed Central - HTML - PubMed

Affiliation: UMR 1313 Génétique Animale et Biologie Intégrative, INRA/AgroParisTech, 78352 Jouy en Josas, France. olympe.chazara@jouy.inra.fr.

ABSTRACT

Background: The LEI0258 marker is located within the B region of the chicken Major Histocompatibility Complex (MHC), and is surprisingly well associated with serology. Therefore, the correlation between the LEI0258 alleles and the MHC class I and the class II alleles at the level of sequences is worth investigating in chickens. Here we describe to which extent the LEI0258 alleles are associated with alleles of classical class I genes and non-classical class II genes, in reference animals as well as local breeds with unknown MHC haplotypes.

Methods: For the class I region, in an exploratory project, we studied 10 animals from 3 breeds: Rhode Island Red, White Leghorn and Fayoumi chickens, by cloning and sequencing B-F1 and B-F2 cDNA from exon 1 to 3'UTR. For the class II region, we reconstructed haplotypes of the 8.8 kb genomic region encompassing three non-classical class II genes: B-DMA, B-DMB1 and B-DMB2, for 146 animals from more than 50 breeds including wild species of jungle fowls.

Results: Overall we found that the LEI0258 marker genotypes gave good indications of the MHC haplotypes, and a very good predictions (>0.95) of the heterozygosity of an animal at the MHC locus.

Conclusions: Our results show that the LEI0258 alleles are strongly associated with haplotypes of classical class I genes and non-classical class II genes, unravelling the reasons why this marker is becoming the reference marker for MHC genotyping in chickens.

No MeSH data available.