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Preliminary association analysis of TLR9 gene polymorphisms and immune parameters in an Italian Holstein calves population.

Catalani E, Nardone A, Barone A, Scicluna MT, Autorino GL, Caprioli A, Lacetera N - BMC Proc (2011)

Bottom Line: For the association study, only results relative to the SNP located in the promoter region have been discussed.Although no significant association was found between genotypes and L, M or H categories, the genotype estimated as the less represented within the population (c) had no calves categorized as H, the highest frequency of L (25%), and mean values of IgG lower (P < 0.005) compared to genotype b.Larger studies are necessary in order to confirm these observations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dipartimento di Produzioni Animali, Università degli Studi della Tuscia, Viterbo, 01100, Italy. nicgio@unitus.it.

ABSTRACT

Background: This preliminary study was aimed at evaluating the association between single nucleotide polymorphisms (SNPs) on Toll like receptor 9 (TLR9) gene and some immunological parameters in a population of Italian Holstein calves.

Methods: The study was carried out in a commercial farm on 68 Holstein calves aging about 6 months. Genomic DNA was extracted from peripheral blood mononuclear cells (PBMC) and genotyped for nine SNPs on TLR9. Immunological parameters considered were the immunoglobulin (Ig) G titers against bovine herpesvirus 1, and the proliferative response of peripheral blood mononuclear cells to mitogens. For the association study, only results relative to the SNP located in the promoter region have been discussed.

Results: Among the nine SNPs expected, only eight were detected. Considering the SNP located in the promoter region, all three possible genotypes were observed, and their distribution was as follows: genotype a (n=34), b (n=19), and c (n=8). On the basis of their response to vaccine, calves were categorized as low (L, n=8), medium (M, n=45) and high responders (H, n=8). Although no significant association was found between genotypes and L, M or H categories, the genotype estimated as the less represented within the population (c) had no calves categorized as H, the highest frequency of L (25%), and mean values of IgG lower (P < 0.005) compared to genotype b. Furthermore, IgG titers were positively correlated with responses of PBMC to mitogens.

Conclusions: Genotype c appeared to be "non advantageous" in terms of immune response. It was characterized by the presence of the mutation in homozygosity and, not surprisingly, it was the most rare genotype in the population. Larger studies are necessary in order to confirm these observations.

No MeSH data available.


Related in: MedlinePlus

Mean values of IgG titers against bovine herpesvirus 1 and proliferative response of PBMC stimulated with concanavalin A (CONA), lipopolysaccharide (LPS) or pokeweed mitogen (PWM) in calves categorized as high (H), medium (M) or low (L) responders. Data are reported as least square means ± standard error.
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Figure 1: Mean values of IgG titers against bovine herpesvirus 1 and proliferative response of PBMC stimulated with concanavalin A (CONA), lipopolysaccharide (LPS) or pokeweed mitogen (PWM) in calves categorized as high (H), medium (M) or low (L) responders. Data are reported as least square means ± standard error.

Mentions: Although no significant association was found between genotypes and H, M, or L categories, genotype c, the less represented within the population, had no calves categorized as H and the highest frequency of L responders (25%), whereas genotype b had the highest frequency of H (21,05%) and the lowest frequency of L responders (5,3%) (Table 1). Furthermore, calves with genotype c had mean values of IgG titers (1.3±0.21) lower (P < 0.005) than their genotype b counterparts (1.7±0.43). Finally, calves categorized as H, M or L showed a similar response in terms of PBMC proliferation, in that PBMC from H, M or L calves showed also the highest, intermediate and lowest proliferative response to mitogens, respectively (Figure 1). In line with these observations, values of IgG titers were positively (P < 0.05) correlated with those relative to proliferation indicating that in the present study the ability to mount a humoral immune response reflected to some extent that involved in developing a cellular response.


Preliminary association analysis of TLR9 gene polymorphisms and immune parameters in an Italian Holstein calves population.

Catalani E, Nardone A, Barone A, Scicluna MT, Autorino GL, Caprioli A, Lacetera N - BMC Proc (2011)

Mean values of IgG titers against bovine herpesvirus 1 and proliferative response of PBMC stimulated with concanavalin A (CONA), lipopolysaccharide (LPS) or pokeweed mitogen (PWM) in calves categorized as high (H), medium (M) or low (L) responders. Data are reported as least square means ± standard error.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108215&req=5

Figure 1: Mean values of IgG titers against bovine herpesvirus 1 and proliferative response of PBMC stimulated with concanavalin A (CONA), lipopolysaccharide (LPS) or pokeweed mitogen (PWM) in calves categorized as high (H), medium (M) or low (L) responders. Data are reported as least square means ± standard error.
Mentions: Although no significant association was found between genotypes and H, M, or L categories, genotype c, the less represented within the population, had no calves categorized as H and the highest frequency of L responders (25%), whereas genotype b had the highest frequency of H (21,05%) and the lowest frequency of L responders (5,3%) (Table 1). Furthermore, calves with genotype c had mean values of IgG titers (1.3±0.21) lower (P < 0.005) than their genotype b counterparts (1.7±0.43). Finally, calves categorized as H, M or L showed a similar response in terms of PBMC proliferation, in that PBMC from H, M or L calves showed also the highest, intermediate and lowest proliferative response to mitogens, respectively (Figure 1). In line with these observations, values of IgG titers were positively (P < 0.05) correlated with those relative to proliferation indicating that in the present study the ability to mount a humoral immune response reflected to some extent that involved in developing a cellular response.

Bottom Line: For the association study, only results relative to the SNP located in the promoter region have been discussed.Although no significant association was found between genotypes and L, M or H categories, the genotype estimated as the less represented within the population (c) had no calves categorized as H, the highest frequency of L (25%), and mean values of IgG lower (P < 0.005) compared to genotype b.Larger studies are necessary in order to confirm these observations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dipartimento di Produzioni Animali, Università degli Studi della Tuscia, Viterbo, 01100, Italy. nicgio@unitus.it.

ABSTRACT

Background: This preliminary study was aimed at evaluating the association between single nucleotide polymorphisms (SNPs) on Toll like receptor 9 (TLR9) gene and some immunological parameters in a population of Italian Holstein calves.

Methods: The study was carried out in a commercial farm on 68 Holstein calves aging about 6 months. Genomic DNA was extracted from peripheral blood mononuclear cells (PBMC) and genotyped for nine SNPs on TLR9. Immunological parameters considered were the immunoglobulin (Ig) G titers against bovine herpesvirus 1, and the proliferative response of peripheral blood mononuclear cells to mitogens. For the association study, only results relative to the SNP located in the promoter region have been discussed.

Results: Among the nine SNPs expected, only eight were detected. Considering the SNP located in the promoter region, all three possible genotypes were observed, and their distribution was as follows: genotype a (n=34), b (n=19), and c (n=8). On the basis of their response to vaccine, calves were categorized as low (L, n=8), medium (M, n=45) and high responders (H, n=8). Although no significant association was found between genotypes and L, M or H categories, the genotype estimated as the less represented within the population (c) had no calves categorized as H, the highest frequency of L (25%), and mean values of IgG lower (P < 0.005) compared to genotype b. Furthermore, IgG titers were positively correlated with responses of PBMC to mitogens.

Conclusions: Genotype c appeared to be "non advantageous" in terms of immune response. It was characterized by the presence of the mutation in homozygosity and, not surprisingly, it was the most rare genotype in the population. Larger studies are necessary in order to confirm these observations.

No MeSH data available.


Related in: MedlinePlus