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Glucose-lowering effect of DLBS3233 is mediated through phosphorylation of tyrosine and upregulation of PPARγ and GLUT4 expression.

Tandrasasmita OM, Wulan DD, Nailufar F, Sinambela J, Tjandrawinata RR - Int J Gen Med (2011)

Bottom Line: DLBS3233 was also found to enhance the expression of genes associated with increased insulin signaling and sensitivity, such as peroxisome proliferator-activated receptor gamma, phosphatidylinositol-3 kinase, Akt, and glucose transporter 4.In addition, glucose transporter 4 protein levels were seen to increase as a result of DLBS3233 administration.DLBS3233, as a combination of herbal extracts, holds promise in the treatment of type 2 diabetes, and possibly also in prevention of the disease.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Pharmacology.

ABSTRACT

Background: DLBS3233 is a standardized extract combination containing Lagerstroemia speciosa and Cinnamomum burmannii. The effect of DLBS3233 on glucose uptake, adiponectin secretion, and insulin signaling was examined in this study.

Methods: 3T3 Swiss albino preadipocytes and adipocytes were used to investigate gene expression detected using the reverse transcription polymerase chain reaction method. Immunoblotting assay and in vitro glucose uptake assay were also carried out in the experiment.

Results: DLBS3233 was seen to increase phosphorylation at the tyrosine residue of the insulin receptor substrate. DLBS3233 was also found to enhance the expression of genes associated with increased insulin signaling and sensitivity, such as peroxisome proliferator-activated receptor gamma, phosphatidylinositol-3 kinase, Akt, and glucose transporter 4. In addition, glucose transporter 4 protein levels were seen to increase as a result of DLBS3233 administration. The combination of extracts also increased glucose uptake and adiponectin secretion, and decreased resistin secretion significantly relative to control cells. Moreover, DLBS3233 administered to insulin-resistant Wistar rats showed an ability to control blood sugar, insulin levels, and other lipoproteins, including high-density lipoprotein, low-density lipoprotein, triglycerides, and total cholesterol.

Conclusion: DLBS3233, as a combination of herbal extracts, holds promise in the treatment of type 2 diabetes, and possibly also in prevention of the disease.

No MeSH data available.


Related in: MedlinePlus

Effect of DLBS3233 9 mg/kg body weight on the levels of glucose (A), postprandial glucose (B), fasting glucose (C), insulin (D), triglycerides (E), total cholesterol (F), low-density lipoprotein (G), and high-density lipoprotein (H) in insulin-resistant Wistar rats, and comparison of fasting glucose and insulin level (I) and further expressed as homeostasis model assessment (J).Notes: Results are mean value ± standard deviation of two independent experiments. *P < 0.05 indicates a significant difference compared with GF group.Abbreviation: GF, glucose and fructose.
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f5-ijgm-4-345: Effect of DLBS3233 9 mg/kg body weight on the levels of glucose (A), postprandial glucose (B), fasting glucose (C), insulin (D), triglycerides (E), total cholesterol (F), low-density lipoprotein (G), and high-density lipoprotein (H) in insulin-resistant Wistar rats, and comparison of fasting glucose and insulin level (I) and further expressed as homeostasis model assessment (J).Notes: Results are mean value ± standard deviation of two independent experiments. *P < 0.05 indicates a significant difference compared with GF group.Abbreviation: GF, glucose and fructose.

Mentions: Treatment with DLBS3233 9 mg/kg body weight for a further 2 weeks achieved a significant decrease in random glucose (by 29.64%, Figure 5A), postprandial glucose (by 30.62%, Figure 5B), and fasting glucose (by 31.41, Figure 5B). Similar results were also found for other biochemical parameters, including for insulin (Figure 5D), triglycerides (Figure 5E), total cholesterol (Figure 5F), and LDL levels (Figure 5G), with decreases of 64.71%, 33.78%, 21.36%, and 30.81%, respectively. The levels of these biochemical parameters were lower when compared with those in the insulin-resistant group, and were approximately equal to those reached in normal conditions. Moreover, HDL levels in insulin-resistant rats treated with DLBS3233 were almost the same as normal, and 18.20% higher compared with levels in the insulin-resistant group (Figure 5H).


Glucose-lowering effect of DLBS3233 is mediated through phosphorylation of tyrosine and upregulation of PPARγ and GLUT4 expression.

Tandrasasmita OM, Wulan DD, Nailufar F, Sinambela J, Tjandrawinata RR - Int J Gen Med (2011)

Effect of DLBS3233 9 mg/kg body weight on the levels of glucose (A), postprandial glucose (B), fasting glucose (C), insulin (D), triglycerides (E), total cholesterol (F), low-density lipoprotein (G), and high-density lipoprotein (H) in insulin-resistant Wistar rats, and comparison of fasting glucose and insulin level (I) and further expressed as homeostasis model assessment (J).Notes: Results are mean value ± standard deviation of two independent experiments. *P < 0.05 indicates a significant difference compared with GF group.Abbreviation: GF, glucose and fructose.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108202&req=5

f5-ijgm-4-345: Effect of DLBS3233 9 mg/kg body weight on the levels of glucose (A), postprandial glucose (B), fasting glucose (C), insulin (D), triglycerides (E), total cholesterol (F), low-density lipoprotein (G), and high-density lipoprotein (H) in insulin-resistant Wistar rats, and comparison of fasting glucose and insulin level (I) and further expressed as homeostasis model assessment (J).Notes: Results are mean value ± standard deviation of two independent experiments. *P < 0.05 indicates a significant difference compared with GF group.Abbreviation: GF, glucose and fructose.
Mentions: Treatment with DLBS3233 9 mg/kg body weight for a further 2 weeks achieved a significant decrease in random glucose (by 29.64%, Figure 5A), postprandial glucose (by 30.62%, Figure 5B), and fasting glucose (by 31.41, Figure 5B). Similar results were also found for other biochemical parameters, including for insulin (Figure 5D), triglycerides (Figure 5E), total cholesterol (Figure 5F), and LDL levels (Figure 5G), with decreases of 64.71%, 33.78%, 21.36%, and 30.81%, respectively. The levels of these biochemical parameters were lower when compared with those in the insulin-resistant group, and were approximately equal to those reached in normal conditions. Moreover, HDL levels in insulin-resistant rats treated with DLBS3233 were almost the same as normal, and 18.20% higher compared with levels in the insulin-resistant group (Figure 5H).

Bottom Line: DLBS3233 was also found to enhance the expression of genes associated with increased insulin signaling and sensitivity, such as peroxisome proliferator-activated receptor gamma, phosphatidylinositol-3 kinase, Akt, and glucose transporter 4.In addition, glucose transporter 4 protein levels were seen to increase as a result of DLBS3233 administration.DLBS3233, as a combination of herbal extracts, holds promise in the treatment of type 2 diabetes, and possibly also in prevention of the disease.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Pharmacology.

ABSTRACT

Background: DLBS3233 is a standardized extract combination containing Lagerstroemia speciosa and Cinnamomum burmannii. The effect of DLBS3233 on glucose uptake, adiponectin secretion, and insulin signaling was examined in this study.

Methods: 3T3 Swiss albino preadipocytes and adipocytes were used to investigate gene expression detected using the reverse transcription polymerase chain reaction method. Immunoblotting assay and in vitro glucose uptake assay were also carried out in the experiment.

Results: DLBS3233 was seen to increase phosphorylation at the tyrosine residue of the insulin receptor substrate. DLBS3233 was also found to enhance the expression of genes associated with increased insulin signaling and sensitivity, such as peroxisome proliferator-activated receptor gamma, phosphatidylinositol-3 kinase, Akt, and glucose transporter 4. In addition, glucose transporter 4 protein levels were seen to increase as a result of DLBS3233 administration. The combination of extracts also increased glucose uptake and adiponectin secretion, and decreased resistin secretion significantly relative to control cells. Moreover, DLBS3233 administered to insulin-resistant Wistar rats showed an ability to control blood sugar, insulin levels, and other lipoproteins, including high-density lipoprotein, low-density lipoprotein, triglycerides, and total cholesterol.

Conclusion: DLBS3233, as a combination of herbal extracts, holds promise in the treatment of type 2 diabetes, and possibly also in prevention of the disease.

No MeSH data available.


Related in: MedlinePlus