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Action of Coriandrum sativum L. Essential Oil upon Oral Candida albicans Biofilm Formation.

Furletti VF, Teixeira IP, Obando-Pereda G, Mardegan RC, Sartoratto A, Figueira GM, Duarte RM, Rehder VL, Duarte MC, Höfling JF - Evid Based Complement Alternat Med (2011)

Bottom Line: The crude oil also affected the biofilm formation in C. albicans causing a decrease in the biofilm growth.Standards of these compounds tested grouped provided a stronger activity than the oil suggesting a synergistic action from the major oil constituents.The activity of C. sativum oil demonstrates its potential for a new natural antifungal formulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Dental School of Piracicaba, University of Campinas-UNICAMP, 13414-903 Piracicaba, SP, Brazil.

ABSTRACT
The efficacy of extracts and essential oils from Allium tuberosum, Coriandrum sativum, Cymbopogon martini, Cymbopogon winterianus, and Santolina chamaecyparissus was evaluated against Candida spp. isolates from the oral cavity of patients with periodontal disease. The most active oil was fractionated and tested against C. albicans biofilm formation. The oils were obtained by water-distillation and the extracts were prepared with macerated dried plant material. The Minimal Inhibitory Concentration-MIC was determined by the microdilution method. Chemical characterization of oil constituents was performed using Gas Chromatography and Mass Spectrometry (GC-MS). C. sativum activity oil upon cell and biofilm morphology was evaluated by Scanning Electron Microscopy (SEM). The best activities against planktonic Candida spp. were observed for the essential oil and the grouped F(8-10) fractions from C. sativum. The crude oil also affected the biofilm formation in C. albicans causing a decrease in the biofilm growth. Chemical analysis of the F(8-10) fractions detected as major active compounds, 2-hexen-1-ol, 3-hexen-1-ol and cyclodecane. Standards of these compounds tested grouped provided a stronger activity than the oil suggesting a synergistic action from the major oil constituents. The activity of C. sativum oil demonstrates its potential for a new natural antifungal formulation.

No MeSH data available.


Related in: MedlinePlus

Biofilm kinetic development: (a) Control, without treatment; (b) action of nystatin, fluconazole and C. sativum essential oil in the biofilm kinetic development for C. albicans CBS 562; (c) action of nystatin, fluconazole and C. sativum essential oil in the biofilm kinetic development for C. albicans clinical isolate 13A5.
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Related In: Results  -  Collection


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fig1: Biofilm kinetic development: (a) Control, without treatment; (b) action of nystatin, fluconazole and C. sativum essential oil in the biofilm kinetic development for C. albicans CBS 562; (c) action of nystatin, fluconazole and C. sativum essential oil in the biofilm kinetic development for C. albicans clinical isolate 13A5.

Mentions: Figure 1(a) shows the kinetic biofilm development for C. albicans CBS 562 and clinical isolate 13A5. The action of nystatin, fluconazole, and C. sativum essential oil upon biofilm growth was presented in Figures 1(b) and 1(c). The crude essential oil from C. sativum and drugs was applied at regular intervals during biofilm cultivation. The results shown in Figures 1(b) and 1(c) indicate a clear effect of the oil on biofilm formation, characterized due to an increase in the lag phase and a decrease in the biofilm growth. Similar effects were observed for nistatin, though inferior to fluconazole (P ≤ .05).


Action of Coriandrum sativum L. Essential Oil upon Oral Candida albicans Biofilm Formation.

Furletti VF, Teixeira IP, Obando-Pereda G, Mardegan RC, Sartoratto A, Figueira GM, Duarte RM, Rehder VL, Duarte MC, Höfling JF - Evid Based Complement Alternat Med (2011)

Biofilm kinetic development: (a) Control, without treatment; (b) action of nystatin, fluconazole and C. sativum essential oil in the biofilm kinetic development for C. albicans CBS 562; (c) action of nystatin, fluconazole and C. sativum essential oil in the biofilm kinetic development for C. albicans clinical isolate 13A5.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108195&req=5

fig1: Biofilm kinetic development: (a) Control, without treatment; (b) action of nystatin, fluconazole and C. sativum essential oil in the biofilm kinetic development for C. albicans CBS 562; (c) action of nystatin, fluconazole and C. sativum essential oil in the biofilm kinetic development for C. albicans clinical isolate 13A5.
Mentions: Figure 1(a) shows the kinetic biofilm development for C. albicans CBS 562 and clinical isolate 13A5. The action of nystatin, fluconazole, and C. sativum essential oil upon biofilm growth was presented in Figures 1(b) and 1(c). The crude essential oil from C. sativum and drugs was applied at regular intervals during biofilm cultivation. The results shown in Figures 1(b) and 1(c) indicate a clear effect of the oil on biofilm formation, characterized due to an increase in the lag phase and a decrease in the biofilm growth. Similar effects were observed for nistatin, though inferior to fluconazole (P ≤ .05).

Bottom Line: The crude oil also affected the biofilm formation in C. albicans causing a decrease in the biofilm growth.Standards of these compounds tested grouped provided a stronger activity than the oil suggesting a synergistic action from the major oil constituents.The activity of C. sativum oil demonstrates its potential for a new natural antifungal formulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Dental School of Piracicaba, University of Campinas-UNICAMP, 13414-903 Piracicaba, SP, Brazil.

ABSTRACT
The efficacy of extracts and essential oils from Allium tuberosum, Coriandrum sativum, Cymbopogon martini, Cymbopogon winterianus, and Santolina chamaecyparissus was evaluated against Candida spp. isolates from the oral cavity of patients with periodontal disease. The most active oil was fractionated and tested against C. albicans biofilm formation. The oils were obtained by water-distillation and the extracts were prepared with macerated dried plant material. The Minimal Inhibitory Concentration-MIC was determined by the microdilution method. Chemical characterization of oil constituents was performed using Gas Chromatography and Mass Spectrometry (GC-MS). C. sativum activity oil upon cell and biofilm morphology was evaluated by Scanning Electron Microscopy (SEM). The best activities against planktonic Candida spp. were observed for the essential oil and the grouped F(8-10) fractions from C. sativum. The crude oil also affected the biofilm formation in C. albicans causing a decrease in the biofilm growth. Chemical analysis of the F(8-10) fractions detected as major active compounds, 2-hexen-1-ol, 3-hexen-1-ol and cyclodecane. Standards of these compounds tested grouped provided a stronger activity than the oil suggesting a synergistic action from the major oil constituents. The activity of C. sativum oil demonstrates its potential for a new natural antifungal formulation.

No MeSH data available.


Related in: MedlinePlus