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Species identification of marine fishes in china with DNA barcoding.

Zhang J - Evid Based Complement Alternat Med (2011)

Bottom Line: There are no overlaps of pairwise genetic variations between conspecific and interspecific comparisons apart from the genera Pampus in which the introgressive hybridization was detected.High efficiency of species identification was demonstrated in the present study by DNA barcoding.Due to the incidence of cryptic species, an assumed threshold is suggested to expedite discovering of new species and biodiversity, especially involving biotas of few studies.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.

ABSTRACT
DNA barcoding is a molecular method that uses a short standardized DNA sequence as a species identification tool. In this study, the standard 652 base-pair region of the mitochondrial cytochrome oxidase subunit I gene (COI) was sequenced in marine fish specimens captured in China. The average genetic distance was 50-fold higher between species than within species, as Kimura two parameter (K2P) genetic distances averaged 15.742% among congeners and only 0.319% for intraspecific individuals. There are no overlaps of pairwise genetic variations between conspecific and interspecific comparisons apart from the genera Pampus in which the introgressive hybridization was detected. High efficiency of species identification was demonstrated in the present study by DNA barcoding. Due to the incidence of cryptic species, an assumed threshold is suggested to expedite discovering of new species and biodiversity, especially involving biotas of few studies.

No MeSH data available.


Boxplot distributions of S, G, and F. Intra-species (S), interspecies among congeneric species (G), and intergenera but intrafamily (F) K2P distances for different families.
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fig2: Boxplot distributions of S, G, and F. Intra-species (S), interspecies among congeneric species (G), and intergenera but intrafamily (F) K2P distances for different families.

Mentions: The mean intraspecies K2P (Kimura two-parameter) distance was 0.319%; the distance increased sharply to 15.742% among individuals of congeneric species. Overall, the average genetic distance among congeneric species is nearly 50-fold higher than that among individuals within species. For the higher taxonomic ranks (family, order, and class), mean pairwise genetic distances increased gradually and reached 20.199%, 24.656%, and 25.225%, respectively (Table 1). Standard errors for K2P genetic distances were small, and values of the mean and median were close within different taxonomic ranks (Table 1). This indicates fluctuations of K2P genetic distances tend to be convergent (Figures 1 and 2).


Species identification of marine fishes in china with DNA barcoding.

Zhang J - Evid Based Complement Alternat Med (2011)

Boxplot distributions of S, G, and F. Intra-species (S), interspecies among congeneric species (G), and intergenera but intrafamily (F) K2P distances for different families.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108176&req=5

fig2: Boxplot distributions of S, G, and F. Intra-species (S), interspecies among congeneric species (G), and intergenera but intrafamily (F) K2P distances for different families.
Mentions: The mean intraspecies K2P (Kimura two-parameter) distance was 0.319%; the distance increased sharply to 15.742% among individuals of congeneric species. Overall, the average genetic distance among congeneric species is nearly 50-fold higher than that among individuals within species. For the higher taxonomic ranks (family, order, and class), mean pairwise genetic distances increased gradually and reached 20.199%, 24.656%, and 25.225%, respectively (Table 1). Standard errors for K2P genetic distances were small, and values of the mean and median were close within different taxonomic ranks (Table 1). This indicates fluctuations of K2P genetic distances tend to be convergent (Figures 1 and 2).

Bottom Line: There are no overlaps of pairwise genetic variations between conspecific and interspecific comparisons apart from the genera Pampus in which the introgressive hybridization was detected.High efficiency of species identification was demonstrated in the present study by DNA barcoding.Due to the incidence of cryptic species, an assumed threshold is suggested to expedite discovering of new species and biodiversity, especially involving biotas of few studies.

View Article: PubMed Central - PubMed

Affiliation: College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.

ABSTRACT
DNA barcoding is a molecular method that uses a short standardized DNA sequence as a species identification tool. In this study, the standard 652 base-pair region of the mitochondrial cytochrome oxidase subunit I gene (COI) was sequenced in marine fish specimens captured in China. The average genetic distance was 50-fold higher between species than within species, as Kimura two parameter (K2P) genetic distances averaged 15.742% among congeners and only 0.319% for intraspecific individuals. There are no overlaps of pairwise genetic variations between conspecific and interspecific comparisons apart from the genera Pampus in which the introgressive hybridization was detected. High efficiency of species identification was demonstrated in the present study by DNA barcoding. Due to the incidence of cryptic species, an assumed threshold is suggested to expedite discovering of new species and biodiversity, especially involving biotas of few studies.

No MeSH data available.