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The comparative protective effects of ganoderma spores lipid and fish oil on N-methyl-N-nitrosourea-induced photoreceptor cell lesion in rats.

Yang G, Xin-Guo D, Na L, Guang-Wei L, Chung PC - Evid Based Complement Alternat Med (2011)

Bottom Line: Purpose.Eyes were obtained at 1, 3, 5, 7, and 10 days.Results.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China.

ABSTRACT
Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P < .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuclei. GSL-treated rats showed significantly elevated a-wave and b-wave amplitudes over MNU group (P < .05) but less than NC group (P < .05) and not significantly different from FO group (P > .05). Conclusion. GSL, like FO, alleviates rat retinal photoreceptor cell damage induced by MNU.

No MeSH data available.


Related in: MedlinePlus

The protection effect of Ganoderma spores lipid (GSL) and fish oil (FO) as shown by electron microscopy on days 1 (a–f), 3 (i–p), and 10 (q–x) for normal control (NC) group (first column) and eyes treated with N-methyl-N-nitrosourea (MNU, second column). Retina in the GSL group (third column) showed milder damage in photoreceptor segments and less nuclear apoptosis than those in MNU group. Retina in the FO group (fourth column) had the same characteristics with those in GLS group. Diamond arrow: pigment epithelium nucleus. Black arrowhead: normal photoreceptor segments. White arrowhead: damaged photoreceptor segments. Black arrow: normal nucleus in the outer nuclear layer. White arrow: apoptosis nucleus in the outer nuclear layer. Round arrow: Bruch's membrane. Scale bar, 1 μm. Magnification: 9700x. These suggest that both GSL and FO treatment can inhibit retinal lesions induced by MNU.
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fig5: The protection effect of Ganoderma spores lipid (GSL) and fish oil (FO) as shown by electron microscopy on days 1 (a–f), 3 (i–p), and 10 (q–x) for normal control (NC) group (first column) and eyes treated with N-methyl-N-nitrosourea (MNU, second column). Retina in the GSL group (third column) showed milder damage in photoreceptor segments and less nuclear apoptosis than those in MNU group. Retina in the FO group (fourth column) had the same characteristics with those in GLS group. Diamond arrow: pigment epithelium nucleus. Black arrowhead: normal photoreceptor segments. White arrowhead: damaged photoreceptor segments. Black arrow: normal nucleus in the outer nuclear layer. White arrow: apoptosis nucleus in the outer nuclear layer. Round arrow: Bruch's membrane. Scale bar, 1 μm. Magnification: 9700x. These suggest that both GSL and FO treatment can inhibit retinal lesions induced by MNU.

Mentions: At all detected time points, photoreceptor segments were intact (Figures 5(a), 5(i), and 5(q)), and photoreceptor cell nuclei were normal in appearance (Figures 5(e), 5(m), and 5(u)) in the NC group. On day 1 after MNU, in the MNU group, photoreceptor segments were severely damaged and being phagocyted (Figure 5(b)), and fragmented photoreceptor cell nuclei were seen (Figure 5(f)). In the GSL group, photoreceptor segments were mainly intact (Figure 5(c)), and photoreceptor cell nuclei were less fragmented than in the MNU group (Figure 5(g)). Similarly, in the FO group, photoreceptor segments were slightly damaged (Figure 5(d)) and photoreceptor cell nuclei were less fragmented than in the MNU group (Figure 5(h)). On day 3 after MNU, photoreceptor segments were completely damaged or lost in the MNU group (Figure 5(j)), and plenty of fragmented photoreceptor cell nuclei were seen (Figure 5(n)). In the GSL group, photoreceptor segments were largely damaged and lost (Figure 5(o)), and some photoreceptor cells were damaged (Figure 5(k)); slightly more damage could be seen in the FO group (Figures 5(l) and 5(p)) than in the GSL group. On day 10 after MNU, photoreceptor segments were completely lost in the MNU group (Figure 5(r)), and the most of the photoreceptor cells were lost (Figure 5(v)). In GSL- (Figures 5(s) and 5(w)) and FO-treated rats (Figures 5(t) and 5(x)), photoreceptor segments were disorganized, and many photoreceptor cells were lost, but no fragmented photoreceptor nuclei could be seen.


The comparative protective effects of ganoderma spores lipid and fish oil on N-methyl-N-nitrosourea-induced photoreceptor cell lesion in rats.

Yang G, Xin-Guo D, Na L, Guang-Wei L, Chung PC - Evid Based Complement Alternat Med (2011)

The protection effect of Ganoderma spores lipid (GSL) and fish oil (FO) as shown by electron microscopy on days 1 (a–f), 3 (i–p), and 10 (q–x) for normal control (NC) group (first column) and eyes treated with N-methyl-N-nitrosourea (MNU, second column). Retina in the GSL group (third column) showed milder damage in photoreceptor segments and less nuclear apoptosis than those in MNU group. Retina in the FO group (fourth column) had the same characteristics with those in GLS group. Diamond arrow: pigment epithelium nucleus. Black arrowhead: normal photoreceptor segments. White arrowhead: damaged photoreceptor segments. Black arrow: normal nucleus in the outer nuclear layer. White arrow: apoptosis nucleus in the outer nuclear layer. Round arrow: Bruch's membrane. Scale bar, 1 μm. Magnification: 9700x. These suggest that both GSL and FO treatment can inhibit retinal lesions induced by MNU.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig5: The protection effect of Ganoderma spores lipid (GSL) and fish oil (FO) as shown by electron microscopy on days 1 (a–f), 3 (i–p), and 10 (q–x) for normal control (NC) group (first column) and eyes treated with N-methyl-N-nitrosourea (MNU, second column). Retina in the GSL group (third column) showed milder damage in photoreceptor segments and less nuclear apoptosis than those in MNU group. Retina in the FO group (fourth column) had the same characteristics with those in GLS group. Diamond arrow: pigment epithelium nucleus. Black arrowhead: normal photoreceptor segments. White arrowhead: damaged photoreceptor segments. Black arrow: normal nucleus in the outer nuclear layer. White arrow: apoptosis nucleus in the outer nuclear layer. Round arrow: Bruch's membrane. Scale bar, 1 μm. Magnification: 9700x. These suggest that both GSL and FO treatment can inhibit retinal lesions induced by MNU.
Mentions: At all detected time points, photoreceptor segments were intact (Figures 5(a), 5(i), and 5(q)), and photoreceptor cell nuclei were normal in appearance (Figures 5(e), 5(m), and 5(u)) in the NC group. On day 1 after MNU, in the MNU group, photoreceptor segments were severely damaged and being phagocyted (Figure 5(b)), and fragmented photoreceptor cell nuclei were seen (Figure 5(f)). In the GSL group, photoreceptor segments were mainly intact (Figure 5(c)), and photoreceptor cell nuclei were less fragmented than in the MNU group (Figure 5(g)). Similarly, in the FO group, photoreceptor segments were slightly damaged (Figure 5(d)) and photoreceptor cell nuclei were less fragmented than in the MNU group (Figure 5(h)). On day 3 after MNU, photoreceptor segments were completely damaged or lost in the MNU group (Figure 5(j)), and plenty of fragmented photoreceptor cell nuclei were seen (Figure 5(n)). In the GSL group, photoreceptor segments were largely damaged and lost (Figure 5(o)), and some photoreceptor cells were damaged (Figure 5(k)); slightly more damage could be seen in the FO group (Figures 5(l) and 5(p)) than in the GSL group. On day 10 after MNU, photoreceptor segments were completely lost in the MNU group (Figure 5(r)), and the most of the photoreceptor cells were lost (Figure 5(v)). In GSL- (Figures 5(s) and 5(w)) and FO-treated rats (Figures 5(t) and 5(x)), photoreceptor segments were disorganized, and many photoreceptor cells were lost, but no fragmented photoreceptor nuclei could be seen.

Bottom Line: Purpose.Eyes were obtained at 1, 3, 5, 7, and 10 days.Results.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China.

ABSTRACT
Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P < .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuclei. GSL-treated rats showed significantly elevated a-wave and b-wave amplitudes over MNU group (P < .05) but less than NC group (P < .05) and not significantly different from FO group (P > .05). Conclusion. GSL, like FO, alleviates rat retinal photoreceptor cell damage induced by MNU.

No MeSH data available.


Related in: MedlinePlus