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The comparative protective effects of ganoderma spores lipid and fish oil on N-methyl-N-nitrosourea-induced photoreceptor cell lesion in rats.

Yang G, Xin-Guo D, Na L, Guang-Wei L, Chung PC - Evid Based Complement Alternat Med (2011)

Bottom Line: Purpose.Eyes were obtained at 1, 3, 5, 7, and 10 days.Results.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China.

ABSTRACT
Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P < .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuclei. GSL-treated rats showed significantly elevated a-wave and b-wave amplitudes over MNU group (P < .05) but less than NC group (P < .05) and not significantly different from FO group (P > .05). Conclusion. GSL, like FO, alleviates rat retinal photoreceptor cell damage induced by MNU.

No MeSH data available.


Related in: MedlinePlus

The relative protection effects of Ganoderma spores lipid (GSL) and fish oil (FO) as reflected by the pathological score of rat retina. (a) Retina pathological scores of rats in the normal control (NC), N-methyl-N-nitrosourea (MNU), GSL, and FO group; (b) scores on day 1; (c) scores on day 3; (d) scores on day 5; (e) scores on day 7; (f) scores on day 10. Retina pathological score was evaluated by histopathology (H-E staining) on days 1, 3, 5, 7, and 10 after MNU injection. Each point represents the score of one rat (right eye). Scores from GSL- and FO-treated eyes were significantly lower than those from MNU-treated eyes on days 3, 5, 7, and 10 after MNU (P < .05). There was no significant difference in scores of GSL- and FO-treated eyes (P > .05). The horizontal bar denotes the average of each group. *P < .05 versus MNU, ***P < .001 versus MNU, ###P < .001 versus NC.
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fig4: The relative protection effects of Ganoderma spores lipid (GSL) and fish oil (FO) as reflected by the pathological score of rat retina. (a) Retina pathological scores of rats in the normal control (NC), N-methyl-N-nitrosourea (MNU), GSL, and FO group; (b) scores on day 1; (c) scores on day 3; (d) scores on day 5; (e) scores on day 7; (f) scores on day 10. Retina pathological score was evaluated by histopathology (H-E staining) on days 1, 3, 5, 7, and 10 after MNU injection. Each point represents the score of one rat (right eye). Scores from GSL- and FO-treated eyes were significantly lower than those from MNU-treated eyes on days 3, 5, 7, and 10 after MNU (P < .05). There was no significant difference in scores of GSL- and FO-treated eyes (P > .05). The horizontal bar denotes the average of each group. *P < .05 versus MNU, ***P < .001 versus MNU, ###P < .001 versus NC.

Mentions: Retina pathological score was evaluated by histopathology (H-E staining) and scored (Figure 4). As shown in Figure 4(a), MNU injection caused significantly higher scores than in the NC group (P < .001). After treatment with GSL, rats developed less retinal pathology than those in the MNU group (P < .001) but still higher than in the NC group (P < .001); disease scores of GSL-treated rats were similar to those of FO treated rats (P = .98 versus GSL group, P < .001 versus MNU group). Meanwhile, retina pathological score was evaluated on days 1, 3, 5, 7, and 10 after MNU injection (Figures 4(b)–4(f)). Scores from GSL- and FO-treated eyes were significantly lower than those of MNU-treated eyes at days 3, 5, 7, and 10 (P < .05). GSL- and FO-treated eyes did not differ significantly in scores (P > .05).


The comparative protective effects of ganoderma spores lipid and fish oil on N-methyl-N-nitrosourea-induced photoreceptor cell lesion in rats.

Yang G, Xin-Guo D, Na L, Guang-Wei L, Chung PC - Evid Based Complement Alternat Med (2011)

The relative protection effects of Ganoderma spores lipid (GSL) and fish oil (FO) as reflected by the pathological score of rat retina. (a) Retina pathological scores of rats in the normal control (NC), N-methyl-N-nitrosourea (MNU), GSL, and FO group; (b) scores on day 1; (c) scores on day 3; (d) scores on day 5; (e) scores on day 7; (f) scores on day 10. Retina pathological score was evaluated by histopathology (H-E staining) on days 1, 3, 5, 7, and 10 after MNU injection. Each point represents the score of one rat (right eye). Scores from GSL- and FO-treated eyes were significantly lower than those from MNU-treated eyes on days 3, 5, 7, and 10 after MNU (P < .05). There was no significant difference in scores of GSL- and FO-treated eyes (P > .05). The horizontal bar denotes the average of each group. *P < .05 versus MNU, ***P < .001 versus MNU, ###P < .001 versus NC.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108162&req=5

fig4: The relative protection effects of Ganoderma spores lipid (GSL) and fish oil (FO) as reflected by the pathological score of rat retina. (a) Retina pathological scores of rats in the normal control (NC), N-methyl-N-nitrosourea (MNU), GSL, and FO group; (b) scores on day 1; (c) scores on day 3; (d) scores on day 5; (e) scores on day 7; (f) scores on day 10. Retina pathological score was evaluated by histopathology (H-E staining) on days 1, 3, 5, 7, and 10 after MNU injection. Each point represents the score of one rat (right eye). Scores from GSL- and FO-treated eyes were significantly lower than those from MNU-treated eyes on days 3, 5, 7, and 10 after MNU (P < .05). There was no significant difference in scores of GSL- and FO-treated eyes (P > .05). The horizontal bar denotes the average of each group. *P < .05 versus MNU, ***P < .001 versus MNU, ###P < .001 versus NC.
Mentions: Retina pathological score was evaluated by histopathology (H-E staining) and scored (Figure 4). As shown in Figure 4(a), MNU injection caused significantly higher scores than in the NC group (P < .001). After treatment with GSL, rats developed less retinal pathology than those in the MNU group (P < .001) but still higher than in the NC group (P < .001); disease scores of GSL-treated rats were similar to those of FO treated rats (P = .98 versus GSL group, P < .001 versus MNU group). Meanwhile, retina pathological score was evaluated on days 1, 3, 5, 7, and 10 after MNU injection (Figures 4(b)–4(f)). Scores from GSL- and FO-treated eyes were significantly lower than those of MNU-treated eyes at days 3, 5, 7, and 10 (P < .05). GSL- and FO-treated eyes did not differ significantly in scores (P > .05).

Bottom Line: Purpose.Eyes were obtained at 1, 3, 5, 7, and 10 days.Results.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China.

ABSTRACT
Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P < .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuclei. GSL-treated rats showed significantly elevated a-wave and b-wave amplitudes over MNU group (P < .05) but less than NC group (P < .05) and not significantly different from FO group (P > .05). Conclusion. GSL, like FO, alleviates rat retinal photoreceptor cell damage induced by MNU.

No MeSH data available.


Related in: MedlinePlus