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The comparative protective effects of ganoderma spores lipid and fish oil on N-methyl-N-nitrosourea-induced photoreceptor cell lesion in rats.

Yang G, Xin-Guo D, Na L, Guang-Wei L, Chung PC - Evid Based Complement Alternat Med (2011)

Bottom Line: Purpose.Eyes were obtained at 1, 3, 5, 7, and 10 days.Results.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China.

ABSTRACT
Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P < .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuclei. GSL-treated rats showed significantly elevated a-wave and b-wave amplitudes over MNU group (P < .05) but less than NC group (P < .05) and not significantly different from FO group (P > .05). Conclusion. GSL, like FO, alleviates rat retinal photoreceptor cell damage induced by MNU.

No MeSH data available.


Related in: MedlinePlus

The H-E staining photographs of rat retina treated with Ganoderma spores lipid (GSL) and fish oil (FO). (a, e, i) Normal control (NC) group on days 1, 3, and 10, respectively; (b, f, j) N-methyl-N-nitrosourea (MNU) treated group on days 1, 3, and 10, respectively; (c, g, k) GSL group on days 1, 3, and 10, respectively; (d, h, l) FO group on days 1, 3, and 10, respectively. A single systemic administration of MNU evoked progressive retinal lesions. GSL and FO treatment caused inhibition of retinal lesions induced by MNU. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer; OS, outer segment. Scale bar, 40 μm.
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fig3: The H-E staining photographs of rat retina treated with Ganoderma spores lipid (GSL) and fish oil (FO). (a, e, i) Normal control (NC) group on days 1, 3, and 10, respectively; (b, f, j) N-methyl-N-nitrosourea (MNU) treated group on days 1, 3, and 10, respectively; (c, g, k) GSL group on days 1, 3, and 10, respectively; (d, h, l) FO group on days 1, 3, and 10, respectively. A single systemic administration of MNU evoked progressive retinal lesions. GSL and FO treatment caused inhibition of retinal lesions induced by MNU. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer; OS, outer segment. Scale bar, 40 μm.

Mentions: A single systemic administration of MNU evoked progressive retinal lesions in all MNU treated rats, while NC rats retained normal retinal appearance. As shown in Figure 3, compared with retinas from the NC group (Figures 3(a), 3(e), and 3(i)) retinas from MNU-treated animals (Figures 3(b), 3(f), and 3(j)) showed an apparent reduction in ONL thickness and distorted nuclear orientation. GSL treatment (Figures 3(c), 3(g), and 3(k)) significantly inhibited the retinal lesions induced by MNU, as did FO treatment (Figures 3(d), 3(h), and 3(l)).


The comparative protective effects of ganoderma spores lipid and fish oil on N-methyl-N-nitrosourea-induced photoreceptor cell lesion in rats.

Yang G, Xin-Guo D, Na L, Guang-Wei L, Chung PC - Evid Based Complement Alternat Med (2011)

The H-E staining photographs of rat retina treated with Ganoderma spores lipid (GSL) and fish oil (FO). (a, e, i) Normal control (NC) group on days 1, 3, and 10, respectively; (b, f, j) N-methyl-N-nitrosourea (MNU) treated group on days 1, 3, and 10, respectively; (c, g, k) GSL group on days 1, 3, and 10, respectively; (d, h, l) FO group on days 1, 3, and 10, respectively. A single systemic administration of MNU evoked progressive retinal lesions. GSL and FO treatment caused inhibition of retinal lesions induced by MNU. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer; OS, outer segment. Scale bar, 40 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3108162&req=5

fig3: The H-E staining photographs of rat retina treated with Ganoderma spores lipid (GSL) and fish oil (FO). (a, e, i) Normal control (NC) group on days 1, 3, and 10, respectively; (b, f, j) N-methyl-N-nitrosourea (MNU) treated group on days 1, 3, and 10, respectively; (c, g, k) GSL group on days 1, 3, and 10, respectively; (d, h, l) FO group on days 1, 3, and 10, respectively. A single systemic administration of MNU evoked progressive retinal lesions. GSL and FO treatment caused inhibition of retinal lesions induced by MNU. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer; OS, outer segment. Scale bar, 40 μm.
Mentions: A single systemic administration of MNU evoked progressive retinal lesions in all MNU treated rats, while NC rats retained normal retinal appearance. As shown in Figure 3, compared with retinas from the NC group (Figures 3(a), 3(e), and 3(i)) retinas from MNU-treated animals (Figures 3(b), 3(f), and 3(j)) showed an apparent reduction in ONL thickness and distorted nuclear orientation. GSL treatment (Figures 3(c), 3(g), and 3(k)) significantly inhibited the retinal lesions induced by MNU, as did FO treatment (Figures 3(d), 3(h), and 3(l)).

Bottom Line: Purpose.Eyes were obtained at 1, 3, 5, 7, and 10 days.Results.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China.

ABSTRACT
Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P < .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuclei. GSL-treated rats showed significantly elevated a-wave and b-wave amplitudes over MNU group (P < .05) but less than NC group (P < .05) and not significantly different from FO group (P > .05). Conclusion. GSL, like FO, alleviates rat retinal photoreceptor cell damage induced by MNU.

No MeSH data available.


Related in: MedlinePlus