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The comparative protective effects of ganoderma spores lipid and fish oil on N-methyl-N-nitrosourea-induced photoreceptor cell lesion in rats.

Yang G, Xin-Guo D, Na L, Guang-Wei L, Chung PC - Evid Based Complement Alternat Med (2011)

Bottom Line: Purpose.Eyes were obtained at 1, 3, 5, 7, and 10 days.Results.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China.

ABSTRACT
Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P < .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuclei. GSL-treated rats showed significantly elevated a-wave and b-wave amplitudes over MNU group (P < .05) but less than NC group (P < .05) and not significantly different from FO group (P > .05). Conclusion. GSL, like FO, alleviates rat retinal photoreceptor cell damage induced by MNU.

No MeSH data available.


Related in: MedlinePlus

The effect of Ganoderma spores lipid (GSL) and fish oil (FO) on the decrease in a- and b-wave amplitude in N-methyl-N-nitrosourea- (MNU-) induced retina damage in rats. (a) A-wave amplitudes of normal control (NC), MNU, GSL, and FO groups; (b) a-wave amplitudes at each time point for NC, MNU, GSL, and FO groups; (c) b-wave amplitudes of NC, MNU, GSL, and FO groups; (d) b-wave amplitudes at each time point for NC, MNU, GSL, and FO groups. After MNU, all eyes showed reductions in both a- and b-wave amplitude compared with the NC group. In rats receiving GSL, both amplitudes decreased significantly less than those of rats in the MNU group, but no significant change was found between the GSL group and the FO group. These data suggest that GSL, similar to FO, has a protective effect on MNU-damaged retinal ERG. *P < .05 versus MNU, **P < .01 versus MNU, #P < .05 versus NC, ##P < .01 versus NC.
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fig2: The effect of Ganoderma spores lipid (GSL) and fish oil (FO) on the decrease in a- and b-wave amplitude in N-methyl-N-nitrosourea- (MNU-) induced retina damage in rats. (a) A-wave amplitudes of normal control (NC), MNU, GSL, and FO groups; (b) a-wave amplitudes at each time point for NC, MNU, GSL, and FO groups; (c) b-wave amplitudes of NC, MNU, GSL, and FO groups; (d) b-wave amplitudes at each time point for NC, MNU, GSL, and FO groups. After MNU, all eyes showed reductions in both a- and b-wave amplitude compared with the NC group. In rats receiving GSL, both amplitudes decreased significantly less than those of rats in the MNU group, but no significant change was found between the GSL group and the FO group. These data suggest that GSL, similar to FO, has a protective effect on MNU-damaged retinal ERG. *P < .05 versus MNU, **P < .01 versus MNU, #P < .05 versus NC, ##P < .01 versus NC.

Mentions: In the MNU group, both a- and b-wave amplitudes were decreased significantly at each time point compared with the NC group (all P < .01, Figures 2(b) and 2(d)). In GSL-treated rats, both a- and b-wave amplitudes were elevated significantly at each time point compared with the MNU group (P < .05, Figures 2(b) and 2(d)) but still lower than the NC group (P < .05 or  .01, Figures 2(b) and 2(d)). Meanwhile, in FO-treated rats, both a- and b-wave amplitudes were basically the same as in the GSL group (P > .05). In brief, GSL-treated eyes exhibited more moderate loss in both a- and b-wave amplitudes compared with MNU control (P < .01, Figures 2(a) and 2(c)), and the loss was statistically significantly similar for the GSL group and FO group (P > .05, Figures 2(a) and 2(c)).


The comparative protective effects of ganoderma spores lipid and fish oil on N-methyl-N-nitrosourea-induced photoreceptor cell lesion in rats.

Yang G, Xin-Guo D, Na L, Guang-Wei L, Chung PC - Evid Based Complement Alternat Med (2011)

The effect of Ganoderma spores lipid (GSL) and fish oil (FO) on the decrease in a- and b-wave amplitude in N-methyl-N-nitrosourea- (MNU-) induced retina damage in rats. (a) A-wave amplitudes of normal control (NC), MNU, GSL, and FO groups; (b) a-wave amplitudes at each time point for NC, MNU, GSL, and FO groups; (c) b-wave amplitudes of NC, MNU, GSL, and FO groups; (d) b-wave amplitudes at each time point for NC, MNU, GSL, and FO groups. After MNU, all eyes showed reductions in both a- and b-wave amplitude compared with the NC group. In rats receiving GSL, both amplitudes decreased significantly less than those of rats in the MNU group, but no significant change was found between the GSL group and the FO group. These data suggest that GSL, similar to FO, has a protective effect on MNU-damaged retinal ERG. *P < .05 versus MNU, **P < .01 versus MNU, #P < .05 versus NC, ##P < .01 versus NC.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108162&req=5

fig2: The effect of Ganoderma spores lipid (GSL) and fish oil (FO) on the decrease in a- and b-wave amplitude in N-methyl-N-nitrosourea- (MNU-) induced retina damage in rats. (a) A-wave amplitudes of normal control (NC), MNU, GSL, and FO groups; (b) a-wave amplitudes at each time point for NC, MNU, GSL, and FO groups; (c) b-wave amplitudes of NC, MNU, GSL, and FO groups; (d) b-wave amplitudes at each time point for NC, MNU, GSL, and FO groups. After MNU, all eyes showed reductions in both a- and b-wave amplitude compared with the NC group. In rats receiving GSL, both amplitudes decreased significantly less than those of rats in the MNU group, but no significant change was found between the GSL group and the FO group. These data suggest that GSL, similar to FO, has a protective effect on MNU-damaged retinal ERG. *P < .05 versus MNU, **P < .01 versus MNU, #P < .05 versus NC, ##P < .01 versus NC.
Mentions: In the MNU group, both a- and b-wave amplitudes were decreased significantly at each time point compared with the NC group (all P < .01, Figures 2(b) and 2(d)). In GSL-treated rats, both a- and b-wave amplitudes were elevated significantly at each time point compared with the MNU group (P < .05, Figures 2(b) and 2(d)) but still lower than the NC group (P < .05 or  .01, Figures 2(b) and 2(d)). Meanwhile, in FO-treated rats, both a- and b-wave amplitudes were basically the same as in the GSL group (P > .05). In brief, GSL-treated eyes exhibited more moderate loss in both a- and b-wave amplitudes compared with MNU control (P < .01, Figures 2(a) and 2(c)), and the loss was statistically significantly similar for the GSL group and FO group (P > .05, Figures 2(a) and 2(c)).

Bottom Line: Purpose.Eyes were obtained at 1, 3, 5, 7, and 10 days.Results.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China.

ABSTRACT
Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P < .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuclei. GSL-treated rats showed significantly elevated a-wave and b-wave amplitudes over MNU group (P < .05) but less than NC group (P < .05) and not significantly different from FO group (P > .05). Conclusion. GSL, like FO, alleviates rat retinal photoreceptor cell damage induced by MNU.

No MeSH data available.


Related in: MedlinePlus