Limits...
The comparative protective effects of ganoderma spores lipid and fish oil on N-methyl-N-nitrosourea-induced photoreceptor cell lesion in rats.

Yang G, Xin-Guo D, Na L, Guang-Wei L, Chung PC - Evid Based Complement Alternat Med (2011)

Bottom Line: Purpose.Eyes were obtained at 1, 3, 5, 7, and 10 days.Results.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China.

ABSTRACT
Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P < .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuclei. GSL-treated rats showed significantly elevated a-wave and b-wave amplitudes over MNU group (P < .05) but less than NC group (P < .05) and not significantly different from FO group (P > .05). Conclusion. GSL, like FO, alleviates rat retinal photoreceptor cell damage induced by MNU.

No MeSH data available.


Related in: MedlinePlus

Schematic representation of animal groups and experimental protocol. Rats were randomly divided into the normal control (NC) group (NC group), the untreated N-methyl-N-nitrosourea (MNU) model control group (MNU group), the Ganoderma spores lipid (GSL) treatment group (GSL group), and the fish oil (FO) treatment group (FO group). We used 0.5% hydroxypropyl methyl cellulose (MC) as the excipient. ▿ Intraperitoneal injection was of 40 mg/kg MNU or 4 mL/kg PBS. Six rats were sacrificed at each time point after MNU or PBS injection.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3108162&req=5

fig1: Schematic representation of animal groups and experimental protocol. Rats were randomly divided into the normal control (NC) group (NC group), the untreated N-methyl-N-nitrosourea (MNU) model control group (MNU group), the Ganoderma spores lipid (GSL) treatment group (GSL group), and the fish oil (FO) treatment group (FO group). We used 0.5% hydroxypropyl methyl cellulose (MC) as the excipient. ▿ Intraperitoneal injection was of 40 mg/kg MNU or 4 mL/kg PBS. Six rats were sacrificed at each time point after MNU or PBS injection.

Mentions: The rats were randomly divided into four groups (Figure 1), including the normal control group (NC group, n = 30), the untreated MNU model control group (MNU group, n = 30), the GSL treatment group (GSL group, n = 30), and the FO treatment group (FO group, n = 30). According to our previous work [12], the dose of GSL (BXLC 070116, specific gravity 0.9173, Holistol International Ltd., Hong Kong, China) used was 2 mg/kg body weight by intragastric administration once daily. GSL was mixed in 1 mL 0.5% hydroxypropyl methyl cellulose (MC) used as the excipient. Intragastric administration of GSL was performed each day for the three days before the animal model was established. One hour after the administration of GSL on day three, a single intraperitoneal injection of 40 mg/kg MNU (Sigma, St. Louis, Mo) was given to establish the animal model. MNU, kept at −20°C in the dark, was dissolved in PBS to a final concentration of 10 mg/mL immediately before use. GSL was consecutively given to animals daily for ten days after MNU injection. In the NC group, rats received intragastric administration of excipient (1 mL once daily) instead of GSL and intraperitoneal injection of 4 ml/kg PBS instead of MNU compared with the GSL group. In the MNU group, the excipient (1 mL once daily) was intragastric administrated instead of GSL. In the FO group, rats received intragastric administration of FO (Incromega DHA 500TG SR, Croda, UK, 2 mg/kg once daily) instead of GSL. One hour after administration of GSL, or FO, or MC on days 1, 3, 5, 7, and 10 post-MNU or PBS injection in each group, the left eye of six randomly selected animals was chosen for electroretinogram (ERG) analysis. The amplitudes of a- and b-waves were analyzed. After ERG analysis, animals were sacrificed, and right eyes were prepared for analysis by light microscopy and electromicroscopy.


The comparative protective effects of ganoderma spores lipid and fish oil on N-methyl-N-nitrosourea-induced photoreceptor cell lesion in rats.

Yang G, Xin-Guo D, Na L, Guang-Wei L, Chung PC - Evid Based Complement Alternat Med (2011)

Schematic representation of animal groups and experimental protocol. Rats were randomly divided into the normal control (NC) group (NC group), the untreated N-methyl-N-nitrosourea (MNU) model control group (MNU group), the Ganoderma spores lipid (GSL) treatment group (GSL group), and the fish oil (FO) treatment group (FO group). We used 0.5% hydroxypropyl methyl cellulose (MC) as the excipient. ▿ Intraperitoneal injection was of 40 mg/kg MNU or 4 mL/kg PBS. Six rats were sacrificed at each time point after MNU or PBS injection.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108162&req=5

fig1: Schematic representation of animal groups and experimental protocol. Rats were randomly divided into the normal control (NC) group (NC group), the untreated N-methyl-N-nitrosourea (MNU) model control group (MNU group), the Ganoderma spores lipid (GSL) treatment group (GSL group), and the fish oil (FO) treatment group (FO group). We used 0.5% hydroxypropyl methyl cellulose (MC) as the excipient. ▿ Intraperitoneal injection was of 40 mg/kg MNU or 4 mL/kg PBS. Six rats were sacrificed at each time point after MNU or PBS injection.
Mentions: The rats were randomly divided into four groups (Figure 1), including the normal control group (NC group, n = 30), the untreated MNU model control group (MNU group, n = 30), the GSL treatment group (GSL group, n = 30), and the FO treatment group (FO group, n = 30). According to our previous work [12], the dose of GSL (BXLC 070116, specific gravity 0.9173, Holistol International Ltd., Hong Kong, China) used was 2 mg/kg body weight by intragastric administration once daily. GSL was mixed in 1 mL 0.5% hydroxypropyl methyl cellulose (MC) used as the excipient. Intragastric administration of GSL was performed each day for the three days before the animal model was established. One hour after the administration of GSL on day three, a single intraperitoneal injection of 40 mg/kg MNU (Sigma, St. Louis, Mo) was given to establish the animal model. MNU, kept at −20°C in the dark, was dissolved in PBS to a final concentration of 10 mg/mL immediately before use. GSL was consecutively given to animals daily for ten days after MNU injection. In the NC group, rats received intragastric administration of excipient (1 mL once daily) instead of GSL and intraperitoneal injection of 4 ml/kg PBS instead of MNU compared with the GSL group. In the MNU group, the excipient (1 mL once daily) was intragastric administrated instead of GSL. In the FO group, rats received intragastric administration of FO (Incromega DHA 500TG SR, Croda, UK, 2 mg/kg once daily) instead of GSL. One hour after administration of GSL, or FO, or MC on days 1, 3, 5, 7, and 10 post-MNU or PBS injection in each group, the left eye of six randomly selected animals was chosen for electroretinogram (ERG) analysis. The amplitudes of a- and b-waves were analyzed. After ERG analysis, animals were sacrificed, and right eyes were prepared for analysis by light microscopy and electromicroscopy.

Bottom Line: Purpose.Eyes were obtained at 1, 3, 5, 7, and 10 days.Results.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road South, Guangzhou 510060, China.

ABSTRACT
Purpose. To compare Ganoderma spores lipid (GSL) and fish oil (FO) in inhibiting retinal photoreceptor cell lesions induced by N-methyl-N-nitrosourea (MNU) in rats. Methods. 120 rats were untreated (normal control, NC group) or treated with a single intraperitoneal injection of 40 mg/kg MNU (MNU group) then treated with GSL (GSL group) or FO (FO group). Eyes were obtained at 1, 3, 5, 7, and 10 days. Results. Light microscopy assay demonstrated that GSL and FO alleviated rat retinal photoreceptor cell damage (GSL and FO versus MNU group P < .001) similarly (GSL versus FO group P = .980). Electron microscopy confirmed that GSL and FO reversed damage to photoreceptor segments and photoreceptor cell nuclei. GSL-treated rats showed significantly elevated a-wave and b-wave amplitudes over MNU group (P < .05) but less than NC group (P < .05) and not significantly different from FO group (P > .05). Conclusion. GSL, like FO, alleviates rat retinal photoreceptor cell damage induced by MNU.

No MeSH data available.


Related in: MedlinePlus