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Effects of vitamin d plus calcium supplements on pharmacokinetics of isoflavones in thai postmenopausal women.

Teekachunhatean S, Pongnad P, Rojanasthein N, Manorot M, Sangdee C - Evid Based Complement Alternat Med (2011)

Bottom Line: The objective of this study was to determine the effects of vitamin D(3) plus calcium supplements (D(3)-calcium) on pharmacokinetics of isoflavones in Thai postmenopausal women.T(max) of daidzein and genistein after regimen B was significantly longer than that of regimen A.Other pharmacokinetic parameters of daidzein and genistein obtained following the three regimens were not significantly different.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.

ABSTRACT
The objective of this study was to determine the effects of vitamin D(3) plus calcium supplements (D(3)-calcium) on pharmacokinetics of isoflavones in Thai postmenopausal women. This study was an open-labeled, randomized three-phase crossover study. Twelve healthy subjects were randomized to receive one of the following regimens: (a) a single dose of isoflavones, (b) a single dose of isoflavones, and D(3)-calcium, or (c) continuous D(3)-calcium for 7 days followed by a single dose of isoflavones on the 8th day. After a washout period, subjects were switched to receive the 2 remaining regimens according to their randomized sequences. Blood samples were collected before dose and at specific time points until 32 hours after isoflavone administration. Plasma was treated with β-glucuronidase/sulfatase to hydrolyze glucuronide and sulfate conjugates of daidzein and genistein. Plasma concentrations of daidzein and genistein were determined by high performance liquid chromatography. The estimated pharmacokinetic parameters of isoflavones were time to maximal plasma concentration (T(max)), maximal plasma concentration (C(max)), half-life (t(1/2)) and area under the plasma concentration-time curve (AUC). T(max) of daidzein and genistein after regimen B was significantly longer than that of regimen A. Other pharmacokinetic parameters of daidzein and genistein obtained following the three regimens were not significantly different.

No MeSH data available.


Mean plasma genistein concentration-time curves after receiving three different regimens obtained from subjects who completed the study without protocol deviation (n = 11). Each point on the curves represents mean plasma concentration at the corresponding time point. Single ISO: a single dose of Flava soy; Single ISO + D3-calcium: a single dose Flava soy and Caltrate 600 + D; Continuous D3-calcium/single ISO: 1 tablet of Caltrate 600 + D twice a day for 7 days, followed by a single dose of Flava soy on the 8th day.
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Related In: Results  -  Collection


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fig3: Mean plasma genistein concentration-time curves after receiving three different regimens obtained from subjects who completed the study without protocol deviation (n = 11). Each point on the curves represents mean plasma concentration at the corresponding time point. Single ISO: a single dose of Flava soy; Single ISO + D3-calcium: a single dose Flava soy and Caltrate 600 + D; Continuous D3-calcium/single ISO: 1 tablet of Caltrate 600 + D twice a day for 7 days, followed by a single dose of Flava soy on the 8th day.

Mentions: The mean plasma concentration-time curves after taking three different regimens of soy extract are shown in Figures 2 and 3. The plasma concentration-time profiles of daidzein and genistein were typically biphasic regardless of soy extract regimens taken. The first and second peak concentrations of both aglycones were generally attained at 2–4 h and 6–8 h, respectively. The mean values of pharmacokinetic parameters of daidzein and genistein after receiving three different regimens are shown in Tables 2 and 3.


Effects of vitamin d plus calcium supplements on pharmacokinetics of isoflavones in thai postmenopausal women.

Teekachunhatean S, Pongnad P, Rojanasthein N, Manorot M, Sangdee C - Evid Based Complement Alternat Med (2011)

Mean plasma genistein concentration-time curves after receiving three different regimens obtained from subjects who completed the study without protocol deviation (n = 11). Each point on the curves represents mean plasma concentration at the corresponding time point. Single ISO: a single dose of Flava soy; Single ISO + D3-calcium: a single dose Flava soy and Caltrate 600 + D; Continuous D3-calcium/single ISO: 1 tablet of Caltrate 600 + D twice a day for 7 days, followed by a single dose of Flava soy on the 8th day.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108160&req=5

fig3: Mean plasma genistein concentration-time curves after receiving three different regimens obtained from subjects who completed the study without protocol deviation (n = 11). Each point on the curves represents mean plasma concentration at the corresponding time point. Single ISO: a single dose of Flava soy; Single ISO + D3-calcium: a single dose Flava soy and Caltrate 600 + D; Continuous D3-calcium/single ISO: 1 tablet of Caltrate 600 + D twice a day for 7 days, followed by a single dose of Flava soy on the 8th day.
Mentions: The mean plasma concentration-time curves after taking three different regimens of soy extract are shown in Figures 2 and 3. The plasma concentration-time profiles of daidzein and genistein were typically biphasic regardless of soy extract regimens taken. The first and second peak concentrations of both aglycones were generally attained at 2–4 h and 6–8 h, respectively. The mean values of pharmacokinetic parameters of daidzein and genistein after receiving three different regimens are shown in Tables 2 and 3.

Bottom Line: The objective of this study was to determine the effects of vitamin D(3) plus calcium supplements (D(3)-calcium) on pharmacokinetics of isoflavones in Thai postmenopausal women.T(max) of daidzein and genistein after regimen B was significantly longer than that of regimen A.Other pharmacokinetic parameters of daidzein and genistein obtained following the three regimens were not significantly different.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.

ABSTRACT
The objective of this study was to determine the effects of vitamin D(3) plus calcium supplements (D(3)-calcium) on pharmacokinetics of isoflavones in Thai postmenopausal women. This study was an open-labeled, randomized three-phase crossover study. Twelve healthy subjects were randomized to receive one of the following regimens: (a) a single dose of isoflavones, (b) a single dose of isoflavones, and D(3)-calcium, or (c) continuous D(3)-calcium for 7 days followed by a single dose of isoflavones on the 8th day. After a washout period, subjects were switched to receive the 2 remaining regimens according to their randomized sequences. Blood samples were collected before dose and at specific time points until 32 hours after isoflavone administration. Plasma was treated with β-glucuronidase/sulfatase to hydrolyze glucuronide and sulfate conjugates of daidzein and genistein. Plasma concentrations of daidzein and genistein were determined by high performance liquid chromatography. The estimated pharmacokinetic parameters of isoflavones were time to maximal plasma concentration (T(max)), maximal plasma concentration (C(max)), half-life (t(1/2)) and area under the plasma concentration-time curve (AUC). T(max) of daidzein and genistein after regimen B was significantly longer than that of regimen A. Other pharmacokinetic parameters of daidzein and genistein obtained following the three regimens were not significantly different.

No MeSH data available.