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Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats.

Kimura M, Shibahara N, Hikiami H, Yoshida T, Jo M, Kaneko M, Nogami T, Fujimoto M, Goto H, Shimada Y - Evid Based Complement Alternat Med (2011)

Bottom Line: Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing.By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I).These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

View Article: PubMed Central - PubMed

Affiliation: Department of Japanese Oriental Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

ABSTRACT
We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500 mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

No MeSH data available.


Related in: MedlinePlus

Expressions of extracellular matrix remodeling factors in skin tissue of pressure ulcer on days 3, 7, 14, and 35 as examined by Western Blotting. (a): α−SMA, (b): TGF-β1, (c): bFGF, (d): collagen III, (e): collagen I. Values are expressed as mean ± SE (n = 4–7). *P < .05, *P < .01 versus control.
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fig11: Expressions of extracellular matrix remodeling factors in skin tissue of pressure ulcer on days 3, 7, 14, and 35 as examined by Western Blotting. (a): α−SMA, (b): TGF-β1, (c): bFGF, (d): collagen III, (e): collagen I. Values are expressed as mean ± SE (n = 4–7). *P < .05, *P < .01 versus control.

Mentions: Extracellular matrix protein: the protein level of α-SMA of the HD-KKT group increased on day 3 and those of the LD-KKT and HD-KKT groups decreased on day 35 compared of that of the control group with statistical significance (Figure 11(a)). The values of TGF-β1 in the HD-KKT group on days 3 and 14 were significantly higher than that of control (Figure 11(b)). The values of bFGF in the LD-KKT and HD-KKT groups were significantly lower than that of control on day 7, and that of the HD-KKT group was higher than that of control on day 35 (Figure 11(c)). The protein levels of collagen III of the LD-KKT and HD-KKT groups were significantly higher than that of control on day 3 (Figure 11(d)). The protein level of collagen I of the LD-KKT group on day 7 and that of the HD-KKT group on day 35 were significantly elevated compared to those of control (Figure 11(e)).


Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats.

Kimura M, Shibahara N, Hikiami H, Yoshida T, Jo M, Kaneko M, Nogami T, Fujimoto M, Goto H, Shimada Y - Evid Based Complement Alternat Med (2011)

Expressions of extracellular matrix remodeling factors in skin tissue of pressure ulcer on days 3, 7, 14, and 35 as examined by Western Blotting. (a): α−SMA, (b): TGF-β1, (c): bFGF, (d): collagen III, (e): collagen I. Values are expressed as mean ± SE (n = 4–7). *P < .05, *P < .01 versus control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108106&req=5

fig11: Expressions of extracellular matrix remodeling factors in skin tissue of pressure ulcer on days 3, 7, 14, and 35 as examined by Western Blotting. (a): α−SMA, (b): TGF-β1, (c): bFGF, (d): collagen III, (e): collagen I. Values are expressed as mean ± SE (n = 4–7). *P < .05, *P < .01 versus control.
Mentions: Extracellular matrix protein: the protein level of α-SMA of the HD-KKT group increased on day 3 and those of the LD-KKT and HD-KKT groups decreased on day 35 compared of that of the control group with statistical significance (Figure 11(a)). The values of TGF-β1 in the HD-KKT group on days 3 and 14 were significantly higher than that of control (Figure 11(b)). The values of bFGF in the LD-KKT and HD-KKT groups were significantly lower than that of control on day 7, and that of the HD-KKT group was higher than that of control on day 35 (Figure 11(c)). The protein levels of collagen III of the LD-KKT and HD-KKT groups were significantly higher than that of control on day 3 (Figure 11(d)). The protein level of collagen I of the LD-KKT group on day 7 and that of the HD-KKT group on day 35 were significantly elevated compared to those of control (Figure 11(e)).

Bottom Line: Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing.By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I).These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

View Article: PubMed Central - PubMed

Affiliation: Department of Japanese Oriental Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

ABSTRACT
We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500 mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

No MeSH data available.


Related in: MedlinePlus