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Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats.

Kimura M, Shibahara N, Hikiami H, Yoshida T, Jo M, Kaneko M, Nogami T, Fujimoto M, Goto H, Shimada Y - Evid Based Complement Alternat Med (2011)

Bottom Line: Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing.By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I).These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

View Article: PubMed Central - PubMed

Affiliation: Department of Japanese Oriental Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

ABSTRACT
We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500 mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

No MeSH data available.


Related in: MedlinePlus

VEGF immunopositive area at pressure ulcer edge (days 3 and 14; scale bar, 100 μm). On day 3, VEGF immunopositive cells were mainly endothelial cells. On day 14, the LD-KKT and HD-KKT groups had more VEGF immunopositive fibroblasts than the control group.
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fig7: VEGF immunopositive area at pressure ulcer edge (days 3 and 14; scale bar, 100 μm). On day 3, VEGF immunopositive cells were mainly endothelial cells. On day 14, the LD-KKT and HD-KKT groups had more VEGF immunopositive fibroblasts than the control group.

Mentions: On day 3, positive reaction to anti-VEGF antibody was observed at the ulcer edge. The necrotic layer, granulation layer, and subcutaneous tissue of the central area showed negative reaction to anti-VEGF antibody, and there was no particular difference among the three groups. On day 14, numerous VEGF immunopositive cells were observed in the granulation tissue of all groups, and in KKT-administered groups more VEGF immunopositive cells were seen compared to the control group (Figure 7). On day 35, VEGF-positive cells were still observed in KKT-administered rats, but had almost disappeared in control rats.


Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats.

Kimura M, Shibahara N, Hikiami H, Yoshida T, Jo M, Kaneko M, Nogami T, Fujimoto M, Goto H, Shimada Y - Evid Based Complement Alternat Med (2011)

VEGF immunopositive area at pressure ulcer edge (days 3 and 14; scale bar, 100 μm). On day 3, VEGF immunopositive cells were mainly endothelial cells. On day 14, the LD-KKT and HD-KKT groups had more VEGF immunopositive fibroblasts than the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108106&req=5

fig7: VEGF immunopositive area at pressure ulcer edge (days 3 and 14; scale bar, 100 μm). On day 3, VEGF immunopositive cells were mainly endothelial cells. On day 14, the LD-KKT and HD-KKT groups had more VEGF immunopositive fibroblasts than the control group.
Mentions: On day 3, positive reaction to anti-VEGF antibody was observed at the ulcer edge. The necrotic layer, granulation layer, and subcutaneous tissue of the central area showed negative reaction to anti-VEGF antibody, and there was no particular difference among the three groups. On day 14, numerous VEGF immunopositive cells were observed in the granulation tissue of all groups, and in KKT-administered groups more VEGF immunopositive cells were seen compared to the control group (Figure 7). On day 35, VEGF-positive cells were still observed in KKT-administered rats, but had almost disappeared in control rats.

Bottom Line: Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing.By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I).These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

View Article: PubMed Central - PubMed

Affiliation: Department of Japanese Oriental Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

ABSTRACT
We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500 mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

No MeSH data available.


Related in: MedlinePlus