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Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats.

Kimura M, Shibahara N, Hikiami H, Yoshida T, Jo M, Kaneko M, Nogami T, Fujimoto M, Goto H, Shimada Y - Evid Based Complement Alternat Med (2011)

Bottom Line: Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing.By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I).These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

View Article: PubMed Central - PubMed

Affiliation: Department of Japanese Oriental Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

ABSTRACT
We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500 mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

No MeSH data available.


Related in: MedlinePlus

Time courses of the areas of pressure ulcers. Closed circles show the area of skin ulcer of control group, open triangles show that of the LD-KKT group, and open circles show that of the HD-KKT group. The areas of skin ulcers of the LD-KKT and HD-KKT groups were smaller than that of the control group on day 1, and there was no difference among the three groups on days 2 to 9. On day 10, days 12 to 16, and days 18 to 24, the areas of skin ulcers of the LD-KKT and HD-KKT groups were smaller than that of the control group. Values are expressed as mean ± SE (n = 6). *P < .05. **P < .01 versus control.
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fig4: Time courses of the areas of pressure ulcers. Closed circles show the area of skin ulcer of control group, open triangles show that of the LD-KKT group, and open circles show that of the HD-KKT group. The areas of skin ulcers of the LD-KKT and HD-KKT groups were smaller than that of the control group on day 1, and there was no difference among the three groups on days 2 to 9. On day 10, days 12 to 16, and days 18 to 24, the areas of skin ulcers of the LD-KKT and HD-KKT groups were smaller than that of the control group. Values are expressed as mean ± SE (n = 6). *P < .05. **P < .01 versus control.

Mentions: The areas of pressure ulcers of the control, LD-KKT, and HD-KKT groups were evaluated each day. Those of the LD-KKT and HD-KKT groups on day 1 were significantly smaller than that of control, and there was no significant difference among them on days 2 to 9. On day 10, days 12 to 16, and days 18 to 24, the areas of the skin ulcers of the LD-KKT and HD-KKT groups were significantly smaller than that of the control group (Figure 4). The number of days until disappearance of skin ulcer in the control group was 26.67 ± 0.42 days, and those of the LD-KKT group (23.50 ± 3.35 days, P < .05) and HD-KKT group (22.33 ± 0.67 days, P < .01) were significantly shortened compared to the control group.


Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats.

Kimura M, Shibahara N, Hikiami H, Yoshida T, Jo M, Kaneko M, Nogami T, Fujimoto M, Goto H, Shimada Y - Evid Based Complement Alternat Med (2011)

Time courses of the areas of pressure ulcers. Closed circles show the area of skin ulcer of control group, open triangles show that of the LD-KKT group, and open circles show that of the HD-KKT group. The areas of skin ulcers of the LD-KKT and HD-KKT groups were smaller than that of the control group on day 1, and there was no difference among the three groups on days 2 to 9. On day 10, days 12 to 16, and days 18 to 24, the areas of skin ulcers of the LD-KKT and HD-KKT groups were smaller than that of the control group. Values are expressed as mean ± SE (n = 6). *P < .05. **P < .01 versus control.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3108106&req=5

fig4: Time courses of the areas of pressure ulcers. Closed circles show the area of skin ulcer of control group, open triangles show that of the LD-KKT group, and open circles show that of the HD-KKT group. The areas of skin ulcers of the LD-KKT and HD-KKT groups were smaller than that of the control group on day 1, and there was no difference among the three groups on days 2 to 9. On day 10, days 12 to 16, and days 18 to 24, the areas of skin ulcers of the LD-KKT and HD-KKT groups were smaller than that of the control group. Values are expressed as mean ± SE (n = 6). *P < .05. **P < .01 versus control.
Mentions: The areas of pressure ulcers of the control, LD-KKT, and HD-KKT groups were evaluated each day. Those of the LD-KKT and HD-KKT groups on day 1 were significantly smaller than that of control, and there was no significant difference among them on days 2 to 9. On day 10, days 12 to 16, and days 18 to 24, the areas of the skin ulcers of the LD-KKT and HD-KKT groups were significantly smaller than that of the control group (Figure 4). The number of days until disappearance of skin ulcer in the control group was 26.67 ± 0.42 days, and those of the LD-KKT group (23.50 ± 3.35 days, P < .05) and HD-KKT group (22.33 ± 0.67 days, P < .01) were significantly shortened compared to the control group.

Bottom Line: Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing.By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I).These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

View Article: PubMed Central - PubMed

Affiliation: Department of Japanese Oriental Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

ABSTRACT
We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500 mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

No MeSH data available.


Related in: MedlinePlus