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Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats.

Kimura M, Shibahara N, Hikiami H, Yoshida T, Jo M, Kaneko M, Nogami T, Fujimoto M, Goto H, Shimada Y - Evid Based Complement Alternat Med (2011)

Bottom Line: Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing.By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I).These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

View Article: PubMed Central - PubMed

Affiliation: Department of Japanese Oriental Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

ABSTRACT
We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500 mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

No MeSH data available.


Related in: MedlinePlus

Photograph of pressure loading. The anesthetized rats were fixed in a lateral position on a wooden fixation plate. As a cushion, a piece of absorbent cotton was placed between the femoral region and the fixation plate, and a stainless steel cylinder weighing 1.02–1.03 kg and measuring 19 mm and 50 cm in diameter and length, respectively, with a silicon stopper measuring 10 mm in diameter, was placed on the skin of the right greater trochanter (1.30 to 1.31 kg/cm2).
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fig2: Photograph of pressure loading. The anesthetized rats were fixed in a lateral position on a wooden fixation plate. As a cushion, a piece of absorbent cotton was placed between the femoral region and the fixation plate, and a stainless steel cylinder weighing 1.02–1.03 kg and measuring 19 mm and 50 cm in diameter and length, respectively, with a silicon stopper measuring 10 mm in diameter, was placed on the skin of the right greater trochanter (1.30 to 1.31 kg/cm2).

Mentions: In this study we adopted a pressure ulcer model of rats [15] using our original modification. After test-keeping for one week, hair from the right greater trochanter of the rat was extensively removed using electric hair clippers and an electric shaver. For anesthesia, sodium pentobarbital at 50 mg/kg was intraperitoneally administered. Before pressure loading, rats assigned to this purpose were anesthetized, and maintenance doses of 40 mg/kg were given at 1 h 15 min and again at 3 h later. If necessary, additional anesthesia (40 mg/kg) was given during pressure loading. The anesthetized rats were fixed in a lateral position on a wooden fixation plate. As a cushion, a piece of absorbent cotton was placed between the femoral region and the fixation plate, and a stainless steel cylinder weighing 1.02–1.03 kg and measuring 19 mm and 50 cm in diameter and length, respectively, with a silicon stopper measuring 10 mm in diameter, was placed on the skin of the right greater trochanter (1.30 to 1.31 kg/cm2) (Figure 2). The duration of pressure loading was 6 h, and pressure loading was performed daily for 4 days.


Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats.

Kimura M, Shibahara N, Hikiami H, Yoshida T, Jo M, Kaneko M, Nogami T, Fujimoto M, Goto H, Shimada Y - Evid Based Complement Alternat Med (2011)

Photograph of pressure loading. The anesthetized rats were fixed in a lateral position on a wooden fixation plate. As a cushion, a piece of absorbent cotton was placed between the femoral region and the fixation plate, and a stainless steel cylinder weighing 1.02–1.03 kg and measuring 19 mm and 50 cm in diameter and length, respectively, with a silicon stopper measuring 10 mm in diameter, was placed on the skin of the right greater trochanter (1.30 to 1.31 kg/cm2).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108106&req=5

fig2: Photograph of pressure loading. The anesthetized rats were fixed in a lateral position on a wooden fixation plate. As a cushion, a piece of absorbent cotton was placed between the femoral region and the fixation plate, and a stainless steel cylinder weighing 1.02–1.03 kg and measuring 19 mm and 50 cm in diameter and length, respectively, with a silicon stopper measuring 10 mm in diameter, was placed on the skin of the right greater trochanter (1.30 to 1.31 kg/cm2).
Mentions: In this study we adopted a pressure ulcer model of rats [15] using our original modification. After test-keeping for one week, hair from the right greater trochanter of the rat was extensively removed using electric hair clippers and an electric shaver. For anesthesia, sodium pentobarbital at 50 mg/kg was intraperitoneally administered. Before pressure loading, rats assigned to this purpose were anesthetized, and maintenance doses of 40 mg/kg were given at 1 h 15 min and again at 3 h later. If necessary, additional anesthesia (40 mg/kg) was given during pressure loading. The anesthetized rats were fixed in a lateral position on a wooden fixation plate. As a cushion, a piece of absorbent cotton was placed between the femoral region and the fixation plate, and a stainless steel cylinder weighing 1.02–1.03 kg and measuring 19 mm and 50 cm in diameter and length, respectively, with a silicon stopper measuring 10 mm in diameter, was placed on the skin of the right greater trochanter (1.30 to 1.31 kg/cm2) (Figure 2). The duration of pressure loading was 6 h, and pressure loading was performed daily for 4 days.

Bottom Line: Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing.By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I).These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

View Article: PubMed Central - PubMed

Affiliation: Department of Japanese Oriental Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

ABSTRACT
We evaluated the effect of kigikenchuto (KKT), a traditional Japanese formula, in a modified rat pressure-loading skin ulcer model. Rats were divided into three groups, KKT extract orally administered (250 or 500 mg/kg/day for 35 days) and control. KKT shortened the duration until healing. Immunohistochemically, KKT increased CD-31-positive vessels in early phase and increased α-smooth muscle actin-(α-SMA-) positive fibroblastic cells in early phase and decreased them in late phase of wound healing. By Western blotting, KKT showed the potential to decrease inflammatory cytokines (MCP-1, IL-1β, and TNF-α) in early phase, decrease vascular endothelial growth factor in early phase and increase it in late phase, and modulate the expression of extracellular protein matrix (α-SMA, TGF-β1, bFGF, collagen III, and collagen I). These results suggested the possibility that KKT accelerates pressure ulcer healing through decreases of inflammatory cytokines, increase of angiogenesis, and induction of extracellular matrix remodeling.

No MeSH data available.


Related in: MedlinePlus