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Aspergillus nidulans transcription factor AtfA interacts with the MAPK SakA to regulate general stress responses, development and spore functions.

Lara-Rojas F, Sánchez O, Kawasaki L, Aguirre J - Mol. Microbiol. (2011)

Bottom Line: Constitutive phosphorylation of SakA induced by the fungicide fludioxonil prevents both, germ tube formation and nuclear division.Similarly, Neurospora crassa SakA orthologue OS-2 is phosphorylated in intact conidia and gets dephosphorylated during germination.We propose that SakA-AtfA interaction regulates gene expression during stress and conidiophore development and that SAPK phosphorylation is a conserved mechanism to regulate transitions between non-growing (spore) and growing (mycelia) states.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biología Celular y Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, 04510, México, D.F., México.

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The deletion of atfA causes derepression of sexual development.A. CLK43 (WT) and TFLΔatfA-02 (ΔatfA) strains were induced to undergo sexual development in confluent plates as previously reported (Kawasaki et al., 2002). Pictures were taken from confluent cultures after 10 days of induction. Under these conditions the ΔatfA mutant produced more Hülle cells and cleistothecia (black spheres; some in circles) than the WT.B. Strains CLK43 (WT) or TFLΔatfA-02 (ΔatfA) were induced to undergo sexual development as (A) and samples were taken every 24 h (see Experimental procedures). The total number of cleistothecia per fixed area was counted under a dissection microscope and used to calculate cleistothecia cm−2 as reported (Kawasaki et al., 2002). Data are mean values from three independent experiments; bars indicate standard deviation.
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fig03: The deletion of atfA causes derepression of sexual development.A. CLK43 (WT) and TFLΔatfA-02 (ΔatfA) strains were induced to undergo sexual development in confluent plates as previously reported (Kawasaki et al., 2002). Pictures were taken from confluent cultures after 10 days of induction. Under these conditions the ΔatfA mutant produced more Hülle cells and cleistothecia (black spheres; some in circles) than the WT.B. Strains CLK43 (WT) or TFLΔatfA-02 (ΔatfA) were induced to undergo sexual development as (A) and samples were taken every 24 h (see Experimental procedures). The total number of cleistothecia per fixed area was counted under a dissection microscope and used to calculate cleistothecia cm−2 as reported (Kawasaki et al., 2002). Data are mean values from three independent experiments; bars indicate standard deviation.

Mentions: Since the inactivation of sakA causes a derepression of sexual development (Kawasaki et al., 2002; Lara-Ortiz et al., 2003), we followed the development of sexual fruiting bodies (cleistothecia) in the ΔatfA mutant. As shown in Fig. 3A and B, the elimination of atfA also resulted in derepressed sexual development and higher numbers of cleistothecia, suggesting that SakA and AtfA repress sexual development through the same pathway.


Aspergillus nidulans transcription factor AtfA interacts with the MAPK SakA to regulate general stress responses, development and spore functions.

Lara-Rojas F, Sánchez O, Kawasaki L, Aguirre J - Mol. Microbiol. (2011)

The deletion of atfA causes derepression of sexual development.A. CLK43 (WT) and TFLΔatfA-02 (ΔatfA) strains were induced to undergo sexual development in confluent plates as previously reported (Kawasaki et al., 2002). Pictures were taken from confluent cultures after 10 days of induction. Under these conditions the ΔatfA mutant produced more Hülle cells and cleistothecia (black spheres; some in circles) than the WT.B. Strains CLK43 (WT) or TFLΔatfA-02 (ΔatfA) were induced to undergo sexual development as (A) and samples were taken every 24 h (see Experimental procedures). The total number of cleistothecia per fixed area was counted under a dissection microscope and used to calculate cleistothecia cm−2 as reported (Kawasaki et al., 2002). Data are mean values from three independent experiments; bars indicate standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108070&req=5

fig03: The deletion of atfA causes derepression of sexual development.A. CLK43 (WT) and TFLΔatfA-02 (ΔatfA) strains were induced to undergo sexual development in confluent plates as previously reported (Kawasaki et al., 2002). Pictures were taken from confluent cultures after 10 days of induction. Under these conditions the ΔatfA mutant produced more Hülle cells and cleistothecia (black spheres; some in circles) than the WT.B. Strains CLK43 (WT) or TFLΔatfA-02 (ΔatfA) were induced to undergo sexual development as (A) and samples were taken every 24 h (see Experimental procedures). The total number of cleistothecia per fixed area was counted under a dissection microscope and used to calculate cleistothecia cm−2 as reported (Kawasaki et al., 2002). Data are mean values from three independent experiments; bars indicate standard deviation.
Mentions: Since the inactivation of sakA causes a derepression of sexual development (Kawasaki et al., 2002; Lara-Ortiz et al., 2003), we followed the development of sexual fruiting bodies (cleistothecia) in the ΔatfA mutant. As shown in Fig. 3A and B, the elimination of atfA also resulted in derepressed sexual development and higher numbers of cleistothecia, suggesting that SakA and AtfA repress sexual development through the same pathway.

Bottom Line: Constitutive phosphorylation of SakA induced by the fungicide fludioxonil prevents both, germ tube formation and nuclear division.Similarly, Neurospora crassa SakA orthologue OS-2 is phosphorylated in intact conidia and gets dephosphorylated during germination.We propose that SakA-AtfA interaction regulates gene expression during stress and conidiophore development and that SAPK phosphorylation is a conserved mechanism to regulate transitions between non-growing (spore) and growing (mycelia) states.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biología Celular y Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, 04510, México, D.F., México.

Show MeSH
Related in: MedlinePlus