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Novel formulation and evaluation of a Q10-loaded solid lipid nanoparticle cream: in vitro and in vivo studies.

Farboud ES, Nasrollahi SA, Tabbakhi Z - Int J Nanomedicine (2011)

Bottom Line: A simple cream of CoQ10 and a cream containing CoQ10-loaded SLNs were prepared and compared on volunteers aged 20-30 years.In vitro release profiles of CoQ10 from simple cream, SLN alone, and CoQ10-loaded SLN cream showed prolonged release for SLNs compared with the simple cream, whereas there was no significant difference between SLN alone and SLN in cream.In vivo skin hydration and elasticity studies on 25 volunteers suggested good dermal penetration and useful activity of Q10 on skin as a hydratant and antiwrinkle cream.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. farbod@sina.tums.ac.ir

ABSTRACT
Solid lipid nanoparticles (SLNs) of coenzyme Q10 (CoQ10) were formulated by a high-pressure homogenization method. The best formulation of SLN dispersion consisted of 13% lipid (cetyl palmitate or stearic acid), 8% surfactant (Tween 80 or Tego Care 450), and water. Stability tests, particle size analysis, differential scanning calorimetry, transmission electron microscopy, and release study were conducted to find the best formulation. A simple cream of CoQ10 and a cream containing CoQ10-loaded SLNs were prepared and compared on volunteers aged 20-30 years. SLNs with particle size between 50 nm and100 nm exhibited the most suitable stability. In vitro release profiles of CoQ10 from simple cream, SLN alone, and CoQ10-loaded SLN cream showed prolonged release for SLNs compared with the simple cream, whereas there was no significant difference between SLN alone and SLN in cream. In vitro release studies also demonstrated that CoQ10-loaded SLN and SLN cream possessed a biphasic release pattern in comparison with simple cream. In vivo skin hydration and elasticity studies on 25 volunteers suggested good dermal penetration and useful activity of Q10 on skin as a hydratant and antiwrinkle cream.

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Related in: MedlinePlus

Percentage of increase in hydration for simple cream and solid lipid nanoparticle (SLN) cream containing Q10 after 1- and 2-month application periods. All results are expressed as values related to the measured values on day 0 and the untreated control (mean ± standard deviation, n = 25; P < 0.05).
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f6-ijn-6-611: Percentage of increase in hydration for simple cream and solid lipid nanoparticle (SLN) cream containing Q10 after 1- and 2-month application periods. All results are expressed as values related to the measured values on day 0 and the untreated control (mean ± standard deviation, n = 25; P < 0.05).

Mentions: After 1 month of topical application, both formulations showed an increase in skin humidity of 40% and 65% for simple cream and SLN cream, respectively. The elasticity of skin was also improved 5% and 120% for simple cream and SLN cream users, respectively. Surprisingly, after 2 months, in simple cream users a 40% increase in skin humidity and 20% elasticity enhancement was observed, whereas in SLN cream users we detected a 120% and 125% increase in skin humidity and elasticity, respectively. The SLN cream profoundly increased both skin humidity and elasticity when compared with simple cream. The results are shown in Figures 6 and 7.


Novel formulation and evaluation of a Q10-loaded solid lipid nanoparticle cream: in vitro and in vivo studies.

Farboud ES, Nasrollahi SA, Tabbakhi Z - Int J Nanomedicine (2011)

Percentage of increase in hydration for simple cream and solid lipid nanoparticle (SLN) cream containing Q10 after 1- and 2-month application periods. All results are expressed as values related to the measured values on day 0 and the untreated control (mean ± standard deviation, n = 25; P < 0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3107720&req=5

f6-ijn-6-611: Percentage of increase in hydration for simple cream and solid lipid nanoparticle (SLN) cream containing Q10 after 1- and 2-month application periods. All results are expressed as values related to the measured values on day 0 and the untreated control (mean ± standard deviation, n = 25; P < 0.05).
Mentions: After 1 month of topical application, both formulations showed an increase in skin humidity of 40% and 65% for simple cream and SLN cream, respectively. The elasticity of skin was also improved 5% and 120% for simple cream and SLN cream users, respectively. Surprisingly, after 2 months, in simple cream users a 40% increase in skin humidity and 20% elasticity enhancement was observed, whereas in SLN cream users we detected a 120% and 125% increase in skin humidity and elasticity, respectively. The SLN cream profoundly increased both skin humidity and elasticity when compared with simple cream. The results are shown in Figures 6 and 7.

Bottom Line: A simple cream of CoQ10 and a cream containing CoQ10-loaded SLNs were prepared and compared on volunteers aged 20-30 years.In vitro release profiles of CoQ10 from simple cream, SLN alone, and CoQ10-loaded SLN cream showed prolonged release for SLNs compared with the simple cream, whereas there was no significant difference between SLN alone and SLN in cream.In vivo skin hydration and elasticity studies on 25 volunteers suggested good dermal penetration and useful activity of Q10 on skin as a hydratant and antiwrinkle cream.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. farbod@sina.tums.ac.ir

ABSTRACT
Solid lipid nanoparticles (SLNs) of coenzyme Q10 (CoQ10) were formulated by a high-pressure homogenization method. The best formulation of SLN dispersion consisted of 13% lipid (cetyl palmitate or stearic acid), 8% surfactant (Tween 80 or Tego Care 450), and water. Stability tests, particle size analysis, differential scanning calorimetry, transmission electron microscopy, and release study were conducted to find the best formulation. A simple cream of CoQ10 and a cream containing CoQ10-loaded SLNs were prepared and compared on volunteers aged 20-30 years. SLNs with particle size between 50 nm and100 nm exhibited the most suitable stability. In vitro release profiles of CoQ10 from simple cream, SLN alone, and CoQ10-loaded SLN cream showed prolonged release for SLNs compared with the simple cream, whereas there was no significant difference between SLN alone and SLN in cream. In vitro release studies also demonstrated that CoQ10-loaded SLN and SLN cream possessed a biphasic release pattern in comparison with simple cream. In vivo skin hydration and elasticity studies on 25 volunteers suggested good dermal penetration and useful activity of Q10 on skin as a hydratant and antiwrinkle cream.

Show MeSH
Related in: MedlinePlus