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The changes of T lymphocytes and cytokines in ICR mice fed with Fe3O4 magnetic nanoparticles.

Wang J, Chen B, Jin N, Xia G, Chen Y, Zhou Y, Cai X, Ding J, Li X, Wang X - Int J Nanomedicine (2011)

Bottom Line: The Fe(3)O(4)-MNPs were synthesized, and their characteristics such as particle size, zeta potential, and X-ray diffraction patterns were measured and determined.Our results indicated that there were no significant differences in splenocyte proliferation and release of cytokines between exposed and control groups.Furthermore, there was no significant difference in the proportions of T-lymphocyte subsets in the low-dose Fe(3)O(4)-MNPs group when compared to the control group, but the proportions of CD3(+)CD4(+) and CD3(+)CD8(+) T-lymphocyte subsets both in the medium- and high-dose Fe(3)O(4)-MNPs groups were higher than those in the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, Zhongda Hospital, Southeast University, Nanjing, People’s Republic of China.

ABSTRACT
The aim of this article is to study the changes inhibited T lymphocytes and cytokines related to the cellular immunity in ICR (imprinting control region) mice fed with Fe(3)O(4) magnetic nanoparticles (Fe(3)O(4)-MNPs). The Fe(3)O(4)-MNPs were synthesized, and their characteristics such as particle size, zeta potential, and X-ray diffraction patterns were measured and determined. All ICR mice were sacrificed after being exposed to 0, 300, 600, and 1200 mg/kg of Fe(3)O(4)-MNPs by single gastric administration for 14 days. Splenocytes proliferation was indicated with stimulate index by MTT assay; release of cytokines in the serum of ICR mice was detected by enzyme-linked immunosorbent assay, and the phenotypic analyses of T-lymphocyte subsets were performed using flow cytometry. Our results indicated that there were no significant differences in splenocyte proliferation and release of cytokines between exposed and control groups. Furthermore, there was no significant difference in the proportions of T-lymphocyte subsets in the low-dose Fe(3)O(4)-MNPs group when compared to the control group, but the proportions of CD3(+)CD4(+) and CD3(+)CD8(+) T-lymphocyte subsets both in the medium- and high-dose Fe(3)O(4)-MNPs groups were higher than those in the control group. It is concluded that a high dose of Fe(3)O(4)-MNPs, to some extent, could influence in vivo immune function of normal ICR mice.

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Splenocytes proliferation of ICR mice fed with different doses of Fe3O4-MNPs.Notes: A Control group, B low-dose group (300 mg/kg Fe3O4-MNPs), C medium-dose group (600 mg/kg Fe3O4-MNPs), D high-dose group (1200 mg/kg Fe3O4-MNPs), *P > 0.05, there were no difference between exposed groups and control group.Abbreviations: Fe3O4-MNPs, magnetic Fe3O4 nanoparticles; ICR, imprinting control region.
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f3-ijn-6-605: Splenocytes proliferation of ICR mice fed with different doses of Fe3O4-MNPs.Notes: A Control group, B low-dose group (300 mg/kg Fe3O4-MNPs), C medium-dose group (600 mg/kg Fe3O4-MNPs), D high-dose group (1200 mg/kg Fe3O4-MNPs), *P > 0.05, there were no difference between exposed groups and control group.Abbreviations: Fe3O4-MNPs, magnetic Fe3O4 nanoparticles; ICR, imprinting control region.

Mentions: Wilcoxon rank test was carried out to compare the data of splenocyte proliferation from the ICR mice in the groups fed with a different dose of Fe3O4-MNPs, which were not subject to the normal distribution. Our results showed that there was no significant difference in SI between the exposed and control groups (P > 0.05) (Figure 3).


The changes of T lymphocytes and cytokines in ICR mice fed with Fe3O4 magnetic nanoparticles.

Wang J, Chen B, Jin N, Xia G, Chen Y, Zhou Y, Cai X, Ding J, Li X, Wang X - Int J Nanomedicine (2011)

Splenocytes proliferation of ICR mice fed with different doses of Fe3O4-MNPs.Notes: A Control group, B low-dose group (300 mg/kg Fe3O4-MNPs), C medium-dose group (600 mg/kg Fe3O4-MNPs), D high-dose group (1200 mg/kg Fe3O4-MNPs), *P > 0.05, there were no difference between exposed groups and control group.Abbreviations: Fe3O4-MNPs, magnetic Fe3O4 nanoparticles; ICR, imprinting control region.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3107719&req=5

f3-ijn-6-605: Splenocytes proliferation of ICR mice fed with different doses of Fe3O4-MNPs.Notes: A Control group, B low-dose group (300 mg/kg Fe3O4-MNPs), C medium-dose group (600 mg/kg Fe3O4-MNPs), D high-dose group (1200 mg/kg Fe3O4-MNPs), *P > 0.05, there were no difference between exposed groups and control group.Abbreviations: Fe3O4-MNPs, magnetic Fe3O4 nanoparticles; ICR, imprinting control region.
Mentions: Wilcoxon rank test was carried out to compare the data of splenocyte proliferation from the ICR mice in the groups fed with a different dose of Fe3O4-MNPs, which were not subject to the normal distribution. Our results showed that there was no significant difference in SI between the exposed and control groups (P > 0.05) (Figure 3).

Bottom Line: The Fe(3)O(4)-MNPs were synthesized, and their characteristics such as particle size, zeta potential, and X-ray diffraction patterns were measured and determined.Our results indicated that there were no significant differences in splenocyte proliferation and release of cytokines between exposed and control groups.Furthermore, there was no significant difference in the proportions of T-lymphocyte subsets in the low-dose Fe(3)O(4)-MNPs group when compared to the control group, but the proportions of CD3(+)CD4(+) and CD3(+)CD8(+) T-lymphocyte subsets both in the medium- and high-dose Fe(3)O(4)-MNPs groups were higher than those in the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, Zhongda Hospital, Southeast University, Nanjing, People’s Republic of China.

ABSTRACT
The aim of this article is to study the changes inhibited T lymphocytes and cytokines related to the cellular immunity in ICR (imprinting control region) mice fed with Fe(3)O(4) magnetic nanoparticles (Fe(3)O(4)-MNPs). The Fe(3)O(4)-MNPs were synthesized, and their characteristics such as particle size, zeta potential, and X-ray diffraction patterns were measured and determined. All ICR mice were sacrificed after being exposed to 0, 300, 600, and 1200 mg/kg of Fe(3)O(4)-MNPs by single gastric administration for 14 days. Splenocytes proliferation was indicated with stimulate index by MTT assay; release of cytokines in the serum of ICR mice was detected by enzyme-linked immunosorbent assay, and the phenotypic analyses of T-lymphocyte subsets were performed using flow cytometry. Our results indicated that there were no significant differences in splenocyte proliferation and release of cytokines between exposed and control groups. Furthermore, there was no significant difference in the proportions of T-lymphocyte subsets in the low-dose Fe(3)O(4)-MNPs group when compared to the control group, but the proportions of CD3(+)CD4(+) and CD3(+)CD8(+) T-lymphocyte subsets both in the medium- and high-dose Fe(3)O(4)-MNPs groups were higher than those in the control group. It is concluded that a high dose of Fe(3)O(4)-MNPs, to some extent, could influence in vivo immune function of normal ICR mice.

Show MeSH
Related in: MedlinePlus