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RGD targeting of human ferritin iron oxide nanoparticles can enhance in vivo carotid MRI of experimental atherosclerosis

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Human ferritin (HFn) is a promising nanoscale protein cage platform for molecular/cellular imaging, and we have developed engineered HFn nanoparticles as MRI agents... To evaluate RGD-conjugated HFn-iron oxide nanoparticles for enhanced in vivo MRI detection of murine carotid atherosclerosis... Mice were then injected with either RGD (n=7) or RGD (n=7) HFn nanoparticles, followed by MRI at 24 and 48 hours post injection... The nanoparticle accumulation was assessed by measuring the extent of T2*-induced reduction in carotid lumen size (% reduction of carotid lumen area)... Perl’s iron staining was performed to identify accumulation of the nanoparticles in the carotid lesions... Both RGD and RGD HFn nanoparticles caused a reduction in lumen size of the ligated left carotid arteries at 48 hrs due to T2* signal loss (p<0.001 vs. preinjection, Figures 1, 2), but the effect was significantly greater with RGD HFn (p=0.01 vs... RGD HFn)... There was no significant lumen reduction in the non-ligated (control) right carotid arteries... Perl’s iron staining confirmed greater accumulation of RGD HFn in the lesion compared to RGD HFn, primarily in neointimal macrophages (Figure 3)... Human ferritin protein cage is a versatile nanoparticle imaging platform for in vivo cellular/molecular MRI, with enhanced atherosclerosis imaging through multivalent RGD targeting.

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Mentions: Both RGD+ and RGD- HFn nanoparticles caused a reduction in lumen size of the ligated left carotid arteries at 48 hrs due to T2* signal loss (p<0.001 vs. preinjection, Figures 1, 2), but the effect was significantly greater with RGD+ HFn (p=0.01 vs. RGD- HFn). There was no significant lumen reduction in the non-ligated (control) right carotid arteries. Perl’s iron staining confirmed greater accumulation of RGD+ HFn in the lesion compared to RGD- HFn, primarily in neointimal macrophages (Figure 3).


RGD targeting of human ferritin iron oxide nanoparticles can enhance in vivo carotid MRI of experimental atherosclerosis
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3106541&req=5

Mentions: Both RGD+ and RGD- HFn nanoparticles caused a reduction in lumen size of the ligated left carotid arteries at 48 hrs due to T2* signal loss (p<0.001 vs. preinjection, Figures 1, 2), but the effect was significantly greater with RGD+ HFn (p=0.01 vs. RGD- HFn). There was no significant lumen reduction in the non-ligated (control) right carotid arteries. Perl’s iron staining confirmed greater accumulation of RGD+ HFn in the lesion compared to RGD- HFn, primarily in neointimal macrophages (Figure 3).

View Article: PubMed Central - HTML

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Human ferritin (HFn) is a promising nanoscale protein cage platform for molecular/cellular imaging, and we have developed engineered HFn nanoparticles as MRI agents... To evaluate RGD-conjugated HFn-iron oxide nanoparticles for enhanced in vivo MRI detection of murine carotid atherosclerosis... Mice were then injected with either RGD (n=7) or RGD (n=7) HFn nanoparticles, followed by MRI at 24 and 48 hours post injection... The nanoparticle accumulation was assessed by measuring the extent of T2*-induced reduction in carotid lumen size (% reduction of carotid lumen area)... Perl’s iron staining was performed to identify accumulation of the nanoparticles in the carotid lesions... Both RGD and RGD HFn nanoparticles caused a reduction in lumen size of the ligated left carotid arteries at 48 hrs due to T2* signal loss (p<0.001 vs. preinjection, Figures 1, 2), but the effect was significantly greater with RGD HFn (p=0.01 vs... RGD HFn)... There was no significant lumen reduction in the non-ligated (control) right carotid arteries... Perl’s iron staining confirmed greater accumulation of RGD HFn in the lesion compared to RGD HFn, primarily in neointimal macrophages (Figure 3)... Human ferritin protein cage is a versatile nanoparticle imaging platform for in vivo cellular/molecular MRI, with enhanced atherosclerosis imaging through multivalent RGD targeting.

No MeSH data available.