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RGD targeting of human ferritin iron oxide nanoparticles can enhance in vivo carotid MRI of experimental atherosclerosis

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Human ferritin (HFn) is a promising nanoscale protein cage platform for molecular/cellular imaging, and we have developed engineered HFn nanoparticles as MRI agents... To evaluate RGD-conjugated HFn-iron oxide nanoparticles for enhanced in vivo MRI detection of murine carotid atherosclerosis... Mice were then injected with either RGD (n=7) or RGD (n=7) HFn nanoparticles, followed by MRI at 24 and 48 hours post injection... The nanoparticle accumulation was assessed by measuring the extent of T2*-induced reduction in carotid lumen size (% reduction of carotid lumen area)... Perl’s iron staining was performed to identify accumulation of the nanoparticles in the carotid lesions... Both RGD and RGD HFn nanoparticles caused a reduction in lumen size of the ligated left carotid arteries at 48 hrs due to T2* signal loss (p<0.001 vs. preinjection, Figures 1, 2), but the effect was significantly greater with RGD HFn (p=0.01 vs... RGD HFn)... There was no significant lumen reduction in the non-ligated (control) right carotid arteries... Human ferritin protein cage is a versatile nanoparticle imaging platform for in vivo cellular/molecular MRI, with enhanced atherosclerosis imaging through multivalent RGD targeting.

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Mentions: Both RGD+ and RGD- HFn nanoparticles caused a reduction in lumen size of the ligated left carotid arteries at 48 hrs due to T2* signal loss (p<0.001 vs. preinjection, Figures 1, 2), but the effect was significantly greater with RGD+ HFn (p=0.01 vs. RGD- HFn). There was no significant lumen reduction in the non-ligated (control) right carotid arteries. Perl’s iron staining confirmed greater accumulation of RGD+ HFn in the lesion compared to RGD- HFn, primarily in neointimal macrophages (Figure 3).


RGD targeting of human ferritin iron oxide nanoparticles can enhance in vivo carotid MRI of experimental atherosclerosis
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3106541&req=5

Mentions: Both RGD+ and RGD- HFn nanoparticles caused a reduction in lumen size of the ligated left carotid arteries at 48 hrs due to T2* signal loss (p<0.001 vs. preinjection, Figures 1, 2), but the effect was significantly greater with RGD+ HFn (p=0.01 vs. RGD- HFn). There was no significant lumen reduction in the non-ligated (control) right carotid arteries. Perl’s iron staining confirmed greater accumulation of RGD+ HFn in the lesion compared to RGD- HFn, primarily in neointimal macrophages (Figure 3).

View Article: PubMed Central - HTML

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Human ferritin (HFn) is a promising nanoscale protein cage platform for molecular/cellular imaging, and we have developed engineered HFn nanoparticles as MRI agents... To evaluate RGD-conjugated HFn-iron oxide nanoparticles for enhanced in vivo MRI detection of murine carotid atherosclerosis... Mice were then injected with either RGD (n=7) or RGD (n=7) HFn nanoparticles, followed by MRI at 24 and 48 hours post injection... The nanoparticle accumulation was assessed by measuring the extent of T2*-induced reduction in carotid lumen size (% reduction of carotid lumen area)... Perl’s iron staining was performed to identify accumulation of the nanoparticles in the carotid lesions... Both RGD and RGD HFn nanoparticles caused a reduction in lumen size of the ligated left carotid arteries at 48 hrs due to T2* signal loss (p<0.001 vs. preinjection, Figures 1, 2), but the effect was significantly greater with RGD HFn (p=0.01 vs... RGD HFn)... There was no significant lumen reduction in the non-ligated (control) right carotid arteries... Human ferritin protein cage is a versatile nanoparticle imaging platform for in vivo cellular/molecular MRI, with enhanced atherosclerosis imaging through multivalent RGD targeting.

No MeSH data available.