Limits...
Association of Trypanosoma vivax in extracellular sites with central nervous system lesions and changes in cerebrospinal fluid in experimentally infected goats.

Batista JS, Rodrigues CM, García HA, Bezerra FS, Olinda RG, Teixeira MM, Soto-Blanco B - Vet. Res. (2011)

Bottom Line: CSF analysis revealed the presence of T. vivax for G2.Meningitis and meningoencephalitis were diagnosed in G2.PCR were positive for T. vivax in all the samples tested.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Sciences, Universidade Federal Rural do Semi-árido (UFERSA), BR 110 - Km 47, CEP: 59625-900, Mossoró-RN, Brazil. jaelsoares@hotmail.com.

ABSTRACT
Changes in cerebrospinal fluid (CSF) and anatomical and histopathological central nervous system (CNS) lesions were evaluated, and the presence of Trypanosoma vivax in CNS tissues was investigated through PCR. Twelve adult male goats were divided into three groups (G): G1, infected with T. vivax and evaluated during the acute phase; G2, infected goats evaluated during the chronic phase; and G3, consisting of non-infected goats. Each goat from G1 and G2 was infected with 1.25 × 10(5) trypomastigotes. Cerebrospinal fluid (CSF) analysis and investigation of T. vivax was performed at the 15th day post-infection (dpi) in G1 goats and on the fifth day after the manifestation of nervous system infection signs in G2 goats. All goats were necropsied, and CNS fragments from G1 and G2 goats were evaluated by PCR for the determination of T. vivax. Hyperthermia, anemia and parasitemia were observed from the fifth dpi for G1 and G2, with the highest parasitemia peak between the seventh and 21st dpi. Nervous system infection signs were observed in three G2 goats between the 30th and 35th dpi. CSF analysis revealed the presence of T. vivax for G2. Meningitis and meningoencephalitis were diagnosed in G2. PCR were positive for T. vivax in all the samples tested. In conclusion, T. vivax may reach the nervous tissue resulting in immune response from the host, which is the cause of progressive clinical and pathological manifestations of the CNS in experimentally infected goats.

Show MeSH

Related in: MedlinePlus

Mean parasitemia (×105 Trypanosomas/mL of blood) in goats infected experimentally with T. vivax (G1 and G2) as a function of the experimental period, in dpi.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3105954&req=5

Figure 2: Mean parasitemia (×105 Trypanosomas/mL of blood) in goats infected experimentally with T. vivax (G1 and G2) as a function of the experimental period, in dpi.

Mentions: The presence of T. vivax in blood was observed from the fifth dpi in G1 and G2 animals. The highest peak of parasitemia occurred from the seventh to the 21st dpi (maximum of 112, 5 × 105 Trypanosomes/mL of blood) in G2 animals, followed by a period of low-level parasitemia between the 22nd and the 31st dpi. On the 32nd dpi, there was a new peak of parasitemia (78.8 × 105 Trypanosomes/mL), followed by another reduction in parasitemia, which remained low until the end of the experimental period. The G1 animals showed similar parasitemia values to G2 animals from the first to the fifteenth dpi (Figure 2).


Association of Trypanosoma vivax in extracellular sites with central nervous system lesions and changes in cerebrospinal fluid in experimentally infected goats.

Batista JS, Rodrigues CM, García HA, Bezerra FS, Olinda RG, Teixeira MM, Soto-Blanco B - Vet. Res. (2011)

Mean parasitemia (×105 Trypanosomas/mL of blood) in goats infected experimentally with T. vivax (G1 and G2) as a function of the experimental period, in dpi.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3105954&req=5

Figure 2: Mean parasitemia (×105 Trypanosomas/mL of blood) in goats infected experimentally with T. vivax (G1 and G2) as a function of the experimental period, in dpi.
Mentions: The presence of T. vivax in blood was observed from the fifth dpi in G1 and G2 animals. The highest peak of parasitemia occurred from the seventh to the 21st dpi (maximum of 112, 5 × 105 Trypanosomes/mL of blood) in G2 animals, followed by a period of low-level parasitemia between the 22nd and the 31st dpi. On the 32nd dpi, there was a new peak of parasitemia (78.8 × 105 Trypanosomes/mL), followed by another reduction in parasitemia, which remained low until the end of the experimental period. The G1 animals showed similar parasitemia values to G2 animals from the first to the fifteenth dpi (Figure 2).

Bottom Line: CSF analysis revealed the presence of T. vivax for G2.Meningitis and meningoencephalitis were diagnosed in G2.PCR were positive for T. vivax in all the samples tested.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Sciences, Universidade Federal Rural do Semi-árido (UFERSA), BR 110 - Km 47, CEP: 59625-900, Mossoró-RN, Brazil. jaelsoares@hotmail.com.

ABSTRACT
Changes in cerebrospinal fluid (CSF) and anatomical and histopathological central nervous system (CNS) lesions were evaluated, and the presence of Trypanosoma vivax in CNS tissues was investigated through PCR. Twelve adult male goats were divided into three groups (G): G1, infected with T. vivax and evaluated during the acute phase; G2, infected goats evaluated during the chronic phase; and G3, consisting of non-infected goats. Each goat from G1 and G2 was infected with 1.25 × 10(5) trypomastigotes. Cerebrospinal fluid (CSF) analysis and investigation of T. vivax was performed at the 15th day post-infection (dpi) in G1 goats and on the fifth day after the manifestation of nervous system infection signs in G2 goats. All goats were necropsied, and CNS fragments from G1 and G2 goats were evaluated by PCR for the determination of T. vivax. Hyperthermia, anemia and parasitemia were observed from the fifth dpi for G1 and G2, with the highest parasitemia peak between the seventh and 21st dpi. Nervous system infection signs were observed in three G2 goats between the 30th and 35th dpi. CSF analysis revealed the presence of T. vivax for G2. Meningitis and meningoencephalitis were diagnosed in G2. PCR were positive for T. vivax in all the samples tested. In conclusion, T. vivax may reach the nervous tissue resulting in immune response from the host, which is the cause of progressive clinical and pathological manifestations of the CNS in experimentally infected goats.

Show MeSH
Related in: MedlinePlus