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Association of Trypanosoma vivax in extracellular sites with central nervous system lesions and changes in cerebrospinal fluid in experimentally infected goats.

Batista JS, Rodrigues CM, García HA, Bezerra FS, Olinda RG, Teixeira MM, Soto-Blanco B - Vet. Res. (2011)

Bottom Line: CSF analysis revealed the presence of T. vivax for G2.Meningitis and meningoencephalitis were diagnosed in G2.PCR were positive for T. vivax in all the samples tested.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Sciences, Universidade Federal Rural do Semi-árido (UFERSA), BR 110 - Km 47, CEP: 59625-900, Mossoró-RN, Brazil. jaelsoares@hotmail.com.

ABSTRACT
Changes in cerebrospinal fluid (CSF) and anatomical and histopathological central nervous system (CNS) lesions were evaluated, and the presence of Trypanosoma vivax in CNS tissues was investigated through PCR. Twelve adult male goats were divided into three groups (G): G1, infected with T. vivax and evaluated during the acute phase; G2, infected goats evaluated during the chronic phase; and G3, consisting of non-infected goats. Each goat from G1 and G2 was infected with 1.25 × 10(5) trypomastigotes. Cerebrospinal fluid (CSF) analysis and investigation of T. vivax was performed at the 15th day post-infection (dpi) in G1 goats and on the fifth day after the manifestation of nervous system infection signs in G2 goats. All goats were necropsied, and CNS fragments from G1 and G2 goats were evaluated by PCR for the determination of T. vivax. Hyperthermia, anemia and parasitemia were observed from the fifth dpi for G1 and G2, with the highest parasitemia peak between the seventh and 21st dpi. Nervous system infection signs were observed in three G2 goats between the 30th and 35th dpi. CSF analysis revealed the presence of T. vivax for G2. Meningitis and meningoencephalitis were diagnosed in G2. PCR were positive for T. vivax in all the samples tested. In conclusion, T. vivax may reach the nervous tissue resulting in immune response from the host, which is the cause of progressive clinical and pathological manifestations of the CNS in experimentally infected goats.

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Mean rectal temperature (RT) values of goats infected experimentally with T. vivax (G1 and G2) and non-infected goats (G3) as a function of the experimental period, in dpi.
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Figure 1: Mean rectal temperature (RT) values of goats infected experimentally with T. vivax (G1 and G2) and non-infected goats (G3) as a function of the experimental period, in dpi.

Mentions: In the week that preceded infection, when the animals went through an adaptation period, no clinical abnormalities were observed. Goats from G1 and G2 showed hyperthermia on the fifth dpi, with a maximum average value of 41.3°C. In the goats from G2, this parameter was higher than average values for G3 until the 21st dpi. After this period, there was a gradual reduction in rectal temperature, which remained within the normal range for the species until the 28th dpi (P < 0.05). From the 29th dpi, there was a new increase in this parameter and a significant difference in relation to G3 (Figure 1).


Association of Trypanosoma vivax in extracellular sites with central nervous system lesions and changes in cerebrospinal fluid in experimentally infected goats.

Batista JS, Rodrigues CM, García HA, Bezerra FS, Olinda RG, Teixeira MM, Soto-Blanco B - Vet. Res. (2011)

Mean rectal temperature (RT) values of goats infected experimentally with T. vivax (G1 and G2) and non-infected goats (G3) as a function of the experimental period, in dpi.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3105954&req=5

Figure 1: Mean rectal temperature (RT) values of goats infected experimentally with T. vivax (G1 and G2) and non-infected goats (G3) as a function of the experimental period, in dpi.
Mentions: In the week that preceded infection, when the animals went through an adaptation period, no clinical abnormalities were observed. Goats from G1 and G2 showed hyperthermia on the fifth dpi, with a maximum average value of 41.3°C. In the goats from G2, this parameter was higher than average values for G3 until the 21st dpi. After this period, there was a gradual reduction in rectal temperature, which remained within the normal range for the species until the 28th dpi (P < 0.05). From the 29th dpi, there was a new increase in this parameter and a significant difference in relation to G3 (Figure 1).

Bottom Line: CSF analysis revealed the presence of T. vivax for G2.Meningitis and meningoencephalitis were diagnosed in G2.PCR were positive for T. vivax in all the samples tested.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Sciences, Universidade Federal Rural do Semi-árido (UFERSA), BR 110 - Km 47, CEP: 59625-900, Mossoró-RN, Brazil. jaelsoares@hotmail.com.

ABSTRACT
Changes in cerebrospinal fluid (CSF) and anatomical and histopathological central nervous system (CNS) lesions were evaluated, and the presence of Trypanosoma vivax in CNS tissues was investigated through PCR. Twelve adult male goats were divided into three groups (G): G1, infected with T. vivax and evaluated during the acute phase; G2, infected goats evaluated during the chronic phase; and G3, consisting of non-infected goats. Each goat from G1 and G2 was infected with 1.25 × 10(5) trypomastigotes. Cerebrospinal fluid (CSF) analysis and investigation of T. vivax was performed at the 15th day post-infection (dpi) in G1 goats and on the fifth day after the manifestation of nervous system infection signs in G2 goats. All goats were necropsied, and CNS fragments from G1 and G2 goats were evaluated by PCR for the determination of T. vivax. Hyperthermia, anemia and parasitemia were observed from the fifth dpi for G1 and G2, with the highest parasitemia peak between the seventh and 21st dpi. Nervous system infection signs were observed in three G2 goats between the 30th and 35th dpi. CSF analysis revealed the presence of T. vivax for G2. Meningitis and meningoencephalitis were diagnosed in G2. PCR were positive for T. vivax in all the samples tested. In conclusion, T. vivax may reach the nervous tissue resulting in immune response from the host, which is the cause of progressive clinical and pathological manifestations of the CNS in experimentally infected goats.

Show MeSH
Related in: MedlinePlus