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Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis.

David D, Rajappan LM, Balachandran K, Thulaseedharan JV, Nair AS, Pillai RM - J. Exp. Clin. Cancer Res. (2011)

Bottom Line: Its aberrant activation has been demonstrated to correlate with many types of human malignancy.Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

View Article: PubMed Central - HTML - PubMed

Affiliation: Integrated Cancer Research, Rajiv Gandhi Centre for Biotechnology, Kerala, India.

ABSTRACT

Background: Signal transducer and activator of transcription 3 (STAT3) is a key signaling molecule and a central cytoplasmic transcription factor, implicated in the regulation of growth. Its aberrant activation has been demonstrated to correlate with many types of human malignancy. However, whether constitutive STAT3 signaling plays a key role in the survival and growth of soft-tissue tumors is still unclear and hence needs to be elucidated further. In our study we examined the expression levels of STAT3 and pSTAT3 in different grades of soft tissue tumors and correlated with its clinicopathological characteristics.

Methods: Expression levels of STAT3 and pSTAT3 in soft tissue tumors were studied using Immunohistochemistry, Western blotting and Reverse transcriptase- PCR and correlated with its clinicopathological characteristics using Chi squared or Fisher's exact test and by logistic regression analysis. Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).

Results: Of the 82 soft tissue tumor samples, fifty four (65.8%) showed immunoreactivity for STAT3 and twenty eight (34.1%) for pSTAT3. Expression of STAT3 and pSTAT3 was significantly associated with tumor grade (P < 0.001; P < 0.001), tumor location (P = 0.025; P = 0.027), plane of tumor (P = 0.011; P = 0.006), and tumor necrosis (P = 0.001; P = 0.002). Western blotting and RT-PCR analysis showed increased expression of STAT3 and p-STAT3 as grade of malignancy increased.

Conclusion: These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

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Related in: MedlinePlus

Representative ethidium bromide stained 2% agarose gel showing semiquantitative Reverse Transcriptase polymerase chain reaction (RT-PCR) analysis and quantification of STAT3 (298 bp) mRNA expression at different stages of soft tissue tumors v/s GAPDH (269 bp) (A and B). Lane 1: benign soft tissue tumor; lane 2: intermediate soft tissue tumor; lane 3: malignant soft tissue tumor. A 100-bp ladder was used as a size standard.
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Figure 4: Representative ethidium bromide stained 2% agarose gel showing semiquantitative Reverse Transcriptase polymerase chain reaction (RT-PCR) analysis and quantification of STAT3 (298 bp) mRNA expression at different stages of soft tissue tumors v/s GAPDH (269 bp) (A and B). Lane 1: benign soft tissue tumor; lane 2: intermediate soft tissue tumor; lane 3: malignant soft tissue tumor. A 100-bp ladder was used as a size standard.

Mentions: Reverse transcription -PCR was done to analyze the mRNA level expression of STAT3 in representative soft tissue tumor samples [Figure 4]. A high level expression of STAT3 mRNA was observed in tumor samples. Among the tumor samples, STAT3 mRNA was found to be overexpressed in malignant and intermediate tumors when compared with benign soft tissue tumors [Figure 5]. Together these results indicate that fluctuations observed in STAT3 mRNA expression correlated with its protein level expression.


Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis.

David D, Rajappan LM, Balachandran K, Thulaseedharan JV, Nair AS, Pillai RM - J. Exp. Clin. Cancer Res. (2011)

Representative ethidium bromide stained 2% agarose gel showing semiquantitative Reverse Transcriptase polymerase chain reaction (RT-PCR) analysis and quantification of STAT3 (298 bp) mRNA expression at different stages of soft tissue tumors v/s GAPDH (269 bp) (A and B). Lane 1: benign soft tissue tumor; lane 2: intermediate soft tissue tumor; lane 3: malignant soft tissue tumor. A 100-bp ladder was used as a size standard.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3105950&req=5

Figure 4: Representative ethidium bromide stained 2% agarose gel showing semiquantitative Reverse Transcriptase polymerase chain reaction (RT-PCR) analysis and quantification of STAT3 (298 bp) mRNA expression at different stages of soft tissue tumors v/s GAPDH (269 bp) (A and B). Lane 1: benign soft tissue tumor; lane 2: intermediate soft tissue tumor; lane 3: malignant soft tissue tumor. A 100-bp ladder was used as a size standard.
Mentions: Reverse transcription -PCR was done to analyze the mRNA level expression of STAT3 in representative soft tissue tumor samples [Figure 4]. A high level expression of STAT3 mRNA was observed in tumor samples. Among the tumor samples, STAT3 mRNA was found to be overexpressed in malignant and intermediate tumors when compared with benign soft tissue tumors [Figure 5]. Together these results indicate that fluctuations observed in STAT3 mRNA expression correlated with its protein level expression.

Bottom Line: Its aberrant activation has been demonstrated to correlate with many types of human malignancy.Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

View Article: PubMed Central - HTML - PubMed

Affiliation: Integrated Cancer Research, Rajiv Gandhi Centre for Biotechnology, Kerala, India.

ABSTRACT

Background: Signal transducer and activator of transcription 3 (STAT3) is a key signaling molecule and a central cytoplasmic transcription factor, implicated in the regulation of growth. Its aberrant activation has been demonstrated to correlate with many types of human malignancy. However, whether constitutive STAT3 signaling plays a key role in the survival and growth of soft-tissue tumors is still unclear and hence needs to be elucidated further. In our study we examined the expression levels of STAT3 and pSTAT3 in different grades of soft tissue tumors and correlated with its clinicopathological characteristics.

Methods: Expression levels of STAT3 and pSTAT3 in soft tissue tumors were studied using Immunohistochemistry, Western blotting and Reverse transcriptase- PCR and correlated with its clinicopathological characteristics using Chi squared or Fisher's exact test and by logistic regression analysis. Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).

Results: Of the 82 soft tissue tumor samples, fifty four (65.8%) showed immunoreactivity for STAT3 and twenty eight (34.1%) for pSTAT3. Expression of STAT3 and pSTAT3 was significantly associated with tumor grade (P < 0.001; P < 0.001), tumor location (P = 0.025; P = 0.027), plane of tumor (P = 0.011; P = 0.006), and tumor necrosis (P = 0.001; P = 0.002). Western blotting and RT-PCR analysis showed increased expression of STAT3 and p-STAT3 as grade of malignancy increased.

Conclusion: These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

Show MeSH
Related in: MedlinePlus