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Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis.

David D, Rajappan LM, Balachandran K, Thulaseedharan JV, Nair AS, Pillai RM - J. Exp. Clin. Cancer Res. (2011)

Bottom Line: Its aberrant activation has been demonstrated to correlate with many types of human malignancy.Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

View Article: PubMed Central - HTML - PubMed

Affiliation: Integrated Cancer Research, Rajiv Gandhi Centre for Biotechnology, Kerala, India.

ABSTRACT

Background: Signal transducer and activator of transcription 3 (STAT3) is a key signaling molecule and a central cytoplasmic transcription factor, implicated in the regulation of growth. Its aberrant activation has been demonstrated to correlate with many types of human malignancy. However, whether constitutive STAT3 signaling plays a key role in the survival and growth of soft-tissue tumors is still unclear and hence needs to be elucidated further. In our study we examined the expression levels of STAT3 and pSTAT3 in different grades of soft tissue tumors and correlated with its clinicopathological characteristics.

Methods: Expression levels of STAT3 and pSTAT3 in soft tissue tumors were studied using Immunohistochemistry, Western blotting and Reverse transcriptase- PCR and correlated with its clinicopathological characteristics using Chi squared or Fisher's exact test and by logistic regression analysis. Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).

Results: Of the 82 soft tissue tumor samples, fifty four (65.8%) showed immunoreactivity for STAT3 and twenty eight (34.1%) for pSTAT3. Expression of STAT3 and pSTAT3 was significantly associated with tumor grade (P < 0.001; P < 0.001), tumor location (P = 0.025; P = 0.027), plane of tumor (P = 0.011; P = 0.006), and tumor necrosis (P = 0.001; P = 0.002). Western blotting and RT-PCR analysis showed increased expression of STAT3 and p-STAT3 as grade of malignancy increased.

Conclusion: These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

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Related in: MedlinePlus

Expression of immunohistochemical markers, STAT3 (A, C, E) and p-STAT3 (B, D, F), in benign (A and B); intermediate (C and D); malignant (E and F) soft tissue tumors. The nuclei were counterstained with hematoxylin blue. Image magnifications are 400×.
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Figure 2: Expression of immunohistochemical markers, STAT3 (A, C, E) and p-STAT3 (B, D, F), in benign (A and B); intermediate (C and D); malignant (E and F) soft tissue tumors. The nuclei were counterstained with hematoxylin blue. Image magnifications are 400×.

Mentions: Immunohistochemical staining revealed both cytoplasmic and nuclear localization of STAT3 and pSTAT3 in benign, intermediate, and malignant soft tissue tumors [Figure 2]. Two of 25 benign tumors expressed mild cytoplasmic positivity for STAT3 whereas 6 intermediate tumors exhibited both mild and moderate cytoplasmic positivity for STAT3. Thirty seven of the 46 malignant tumors showed intense STAT3 expression in the cytoplasm whereas the remaining 9 tissues showed moderate and mild cytoplasmic positivity. pSTAT3 expression was not observed in benign tumors. Both mild and moderate cytoplasmic expression of pSTAT3 was observed in intermediate tumors and only malignant tumors exhibited intense cytoplasmic expression for pSTAT3.


Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis.

David D, Rajappan LM, Balachandran K, Thulaseedharan JV, Nair AS, Pillai RM - J. Exp. Clin. Cancer Res. (2011)

Expression of immunohistochemical markers, STAT3 (A, C, E) and p-STAT3 (B, D, F), in benign (A and B); intermediate (C and D); malignant (E and F) soft tissue tumors. The nuclei were counterstained with hematoxylin blue. Image magnifications are 400×.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3105950&req=5

Figure 2: Expression of immunohistochemical markers, STAT3 (A, C, E) and p-STAT3 (B, D, F), in benign (A and B); intermediate (C and D); malignant (E and F) soft tissue tumors. The nuclei were counterstained with hematoxylin blue. Image magnifications are 400×.
Mentions: Immunohistochemical staining revealed both cytoplasmic and nuclear localization of STAT3 and pSTAT3 in benign, intermediate, and malignant soft tissue tumors [Figure 2]. Two of 25 benign tumors expressed mild cytoplasmic positivity for STAT3 whereas 6 intermediate tumors exhibited both mild and moderate cytoplasmic positivity for STAT3. Thirty seven of the 46 malignant tumors showed intense STAT3 expression in the cytoplasm whereas the remaining 9 tissues showed moderate and mild cytoplasmic positivity. pSTAT3 expression was not observed in benign tumors. Both mild and moderate cytoplasmic expression of pSTAT3 was observed in intermediate tumors and only malignant tumors exhibited intense cytoplasmic expression for pSTAT3.

Bottom Line: Its aberrant activation has been demonstrated to correlate with many types of human malignancy.Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

View Article: PubMed Central - HTML - PubMed

Affiliation: Integrated Cancer Research, Rajiv Gandhi Centre for Biotechnology, Kerala, India.

ABSTRACT

Background: Signal transducer and activator of transcription 3 (STAT3) is a key signaling molecule and a central cytoplasmic transcription factor, implicated in the regulation of growth. Its aberrant activation has been demonstrated to correlate with many types of human malignancy. However, whether constitutive STAT3 signaling plays a key role in the survival and growth of soft-tissue tumors is still unclear and hence needs to be elucidated further. In our study we examined the expression levels of STAT3 and pSTAT3 in different grades of soft tissue tumors and correlated with its clinicopathological characteristics.

Methods: Expression levels of STAT3 and pSTAT3 in soft tissue tumors were studied using Immunohistochemistry, Western blotting and Reverse transcriptase- PCR and correlated with its clinicopathological characteristics using Chi squared or Fisher's exact test and by logistic regression analysis. Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).

Results: Of the 82 soft tissue tumor samples, fifty four (65.8%) showed immunoreactivity for STAT3 and twenty eight (34.1%) for pSTAT3. Expression of STAT3 and pSTAT3 was significantly associated with tumor grade (P < 0.001; P < 0.001), tumor location (P = 0.025; P = 0.027), plane of tumor (P = 0.011; P = 0.006), and tumor necrosis (P = 0.001; P = 0.002). Western blotting and RT-PCR analysis showed increased expression of STAT3 and p-STAT3 as grade of malignancy increased.

Conclusion: These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

Show MeSH
Related in: MedlinePlus