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Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis.

David D, Rajappan LM, Balachandran K, Thulaseedharan JV, Nair AS, Pillai RM - J. Exp. Clin. Cancer Res. (2011)

Bottom Line: Its aberrant activation has been demonstrated to correlate with many types of human malignancy.Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

View Article: PubMed Central - HTML - PubMed

Affiliation: Integrated Cancer Research, Rajiv Gandhi Centre for Biotechnology, Kerala, India.

ABSTRACT

Background: Signal transducer and activator of transcription 3 (STAT3) is a key signaling molecule and a central cytoplasmic transcription factor, implicated in the regulation of growth. Its aberrant activation has been demonstrated to correlate with many types of human malignancy. However, whether constitutive STAT3 signaling plays a key role in the survival and growth of soft-tissue tumors is still unclear and hence needs to be elucidated further. In our study we examined the expression levels of STAT3 and pSTAT3 in different grades of soft tissue tumors and correlated with its clinicopathological characteristics.

Methods: Expression levels of STAT3 and pSTAT3 in soft tissue tumors were studied using Immunohistochemistry, Western blotting and Reverse transcriptase- PCR and correlated with its clinicopathological characteristics using Chi squared or Fisher's exact test and by logistic regression analysis. Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).

Results: Of the 82 soft tissue tumor samples, fifty four (65.8%) showed immunoreactivity for STAT3 and twenty eight (34.1%) for pSTAT3. Expression of STAT3 and pSTAT3 was significantly associated with tumor grade (P < 0.001; P < 0.001), tumor location (P = 0.025; P = 0.027), plane of tumor (P = 0.011; P = 0.006), and tumor necrosis (P = 0.001; P = 0.002). Western blotting and RT-PCR analysis showed increased expression of STAT3 and p-STAT3 as grade of malignancy increased.

Conclusion: These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

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Related in: MedlinePlus

Pathologic features of benign, intermediate, and malignant soft tissue tumors. Benign tumor (A) shows cystic degeneration and nuclear palisading and (B) shows nests of granular cells separated by fibrocollagenous tissue. The intermediate grade tumors (C) shows solid, cellular lobules consisting of plump endothelial cells lining tiny rounded vascular spaces with inconspicuous and (D) shows proliferation of spindle cells in inflammatory background. The malignant soft tissue tumors (E) shows epithelioid cells arranged in nests, with a pseudoalveolar pattern and (F) shows lobulated vascular neoplasm composed of small blue round cells in sheets and rosettes. Image magnifications are 400×.
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Figure 1: Pathologic features of benign, intermediate, and malignant soft tissue tumors. Benign tumor (A) shows cystic degeneration and nuclear palisading and (B) shows nests of granular cells separated by fibrocollagenous tissue. The intermediate grade tumors (C) shows solid, cellular lobules consisting of plump endothelial cells lining tiny rounded vascular spaces with inconspicuous and (D) shows proliferation of spindle cells in inflammatory background. The malignant soft tissue tumors (E) shows epithelioid cells arranged in nests, with a pseudoalveolar pattern and (F) shows lobulated vascular neoplasm composed of small blue round cells in sheets and rosettes. Image magnifications are 400×.

Mentions: Among the 82 tumors studied, 38 were well-circumscribed and showed significant association with tumor grade (P < 0.001). Necrosis was studied in all the tumors and significant association was observed with the grade of the tumor (P < 0.001). Tables 1 list the clinicopathological characteristics of the soft tissue tumors selected for the study. Pathologic features of the representative benign, intermediate and malignant soft tissue tumors were given in Figure 1.


Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis.

David D, Rajappan LM, Balachandran K, Thulaseedharan JV, Nair AS, Pillai RM - J. Exp. Clin. Cancer Res. (2011)

Pathologic features of benign, intermediate, and malignant soft tissue tumors. Benign tumor (A) shows cystic degeneration and nuclear palisading and (B) shows nests of granular cells separated by fibrocollagenous tissue. The intermediate grade tumors (C) shows solid, cellular lobules consisting of plump endothelial cells lining tiny rounded vascular spaces with inconspicuous and (D) shows proliferation of spindle cells in inflammatory background. The malignant soft tissue tumors (E) shows epithelioid cells arranged in nests, with a pseudoalveolar pattern and (F) shows lobulated vascular neoplasm composed of small blue round cells in sheets and rosettes. Image magnifications are 400×.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3105950&req=5

Figure 1: Pathologic features of benign, intermediate, and malignant soft tissue tumors. Benign tumor (A) shows cystic degeneration and nuclear palisading and (B) shows nests of granular cells separated by fibrocollagenous tissue. The intermediate grade tumors (C) shows solid, cellular lobules consisting of plump endothelial cells lining tiny rounded vascular spaces with inconspicuous and (D) shows proliferation of spindle cells in inflammatory background. The malignant soft tissue tumors (E) shows epithelioid cells arranged in nests, with a pseudoalveolar pattern and (F) shows lobulated vascular neoplasm composed of small blue round cells in sheets and rosettes. Image magnifications are 400×.
Mentions: Among the 82 tumors studied, 38 were well-circumscribed and showed significant association with tumor grade (P < 0.001). Necrosis was studied in all the tumors and significant association was observed with the grade of the tumor (P < 0.001). Tables 1 list the clinicopathological characteristics of the soft tissue tumors selected for the study. Pathologic features of the representative benign, intermediate and malignant soft tissue tumors were given in Figure 1.

Bottom Line: Its aberrant activation has been demonstrated to correlate with many types of human malignancy.Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

View Article: PubMed Central - HTML - PubMed

Affiliation: Integrated Cancer Research, Rajiv Gandhi Centre for Biotechnology, Kerala, India.

ABSTRACT

Background: Signal transducer and activator of transcription 3 (STAT3) is a key signaling molecule and a central cytoplasmic transcription factor, implicated in the regulation of growth. Its aberrant activation has been demonstrated to correlate with many types of human malignancy. However, whether constitutive STAT3 signaling plays a key role in the survival and growth of soft-tissue tumors is still unclear and hence needs to be elucidated further. In our study we examined the expression levels of STAT3 and pSTAT3 in different grades of soft tissue tumors and correlated with its clinicopathological characteristics.

Methods: Expression levels of STAT3 and pSTAT3 in soft tissue tumors were studied using Immunohistochemistry, Western blotting and Reverse transcriptase- PCR and correlated with its clinicopathological characteristics using Chi squared or Fisher's exact test and by logistic regression analysis. Statistical analysis was done using Intercooled Stata software (Intercooled Stata 8.2 version).

Results: Of the 82 soft tissue tumor samples, fifty four (65.8%) showed immunoreactivity for STAT3 and twenty eight (34.1%) for pSTAT3. Expression of STAT3 and pSTAT3 was significantly associated with tumor grade (P < 0.001; P < 0.001), tumor location (P = 0.025; P = 0.027), plane of tumor (P = 0.011; P = 0.006), and tumor necrosis (P = 0.001; P = 0.002). Western blotting and RT-PCR analysis showed increased expression of STAT3 and p-STAT3 as grade of malignancy increased.

Conclusion: These findings suggest that constitutive activation of STAT3 is an important factor related to carcinogenesis of human soft tissue tumors and is significantly associated with its clinicopathological parameters which may possibly have potential diagnostic implications.

Show MeSH
Related in: MedlinePlus