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Evolution of linear chromosomes and multipartite genomes in yeast mitochondria.

Valach M, Farkas Z, Fricova D, Kovac J, Brejova B, Vinar T, Pfeiffer I, Kucsera J, Tomaska L, Lang BF, Nosek J - Nucleic Acids Res. (2011)

Bottom Line: Our survey revealed a puzzling variability of genome architecture, including circular- and linear-mapping and multipartite linear forms.We propose that the arrangement of large inverted repeats identified in these genomes plays a crucial role in alterations of their molecular architectures.We suggest that molecular transactions generating linear mitochondrial DNA molecules with defined telomeric structures may parallel the evolutionary emergence of linear chromosomes and multipartite genomes in general and may provide clues for the origin of telomeres and pathways implicated in their maintenance.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Comenius University, Mlynska dolina CH-1, 842 15 Bratislava, Slovak republic.

ABSTRACT
Mitochondrial genome diversity in closely related species provides an excellent platform for investigation of chromosome architecture and its evolution by means of comparative genomics. In this study, we determined the complete mitochondrial DNA sequences of eight Candida species and analyzed their molecular architectures. Our survey revealed a puzzling variability of genome architecture, including circular- and linear-mapping and multipartite linear forms. We propose that the arrangement of large inverted repeats identified in these genomes plays a crucial role in alterations of their molecular architectures. In specific arrangements, the inverted repeats appear to function as resolution elements, allowing genome conversion among different topologies, eventually leading to genome fragmentation into multiple linear DNA molecules. We suggest that molecular transactions generating linear mitochondrial DNA molecules with defined telomeric structures may parallel the evolutionary emergence of linear chromosomes and multipartite genomes in general and may provide clues for the origin of telomeres and pathways implicated in their maintenance.

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Comparison of mitochondrial gene orders among species from C. neerlandica–C. tropicalis (A), L. elongisporus–C. parapsilosis (B) and C. subhashii–C. alai (C) lineages. Individual genes and blocks with conserved gene order are shown by identical colors. Duplicated regions are framed. The symbols wedge and caret indicate the orientation of genes, TEL (telomeres) and LIR. In L. elongisporus, both LIRs (LIR*) consist of two regions of ∼4 kb separated by 574 and 95 bp-long unique sequences.
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Figure 5: Comparison of mitochondrial gene orders among species from C. neerlandica–C. tropicalis (A), L. elongisporus–C. parapsilosis (B) and C. subhashii–C. alai (C) lineages. Individual genes and blocks with conserved gene order are shown by identical colors. Duplicated regions are framed. The symbols wedge and caret indicate the orientation of genes, TEL (telomeres) and LIR. In L. elongisporus, both LIRs (LIR*) consist of two regions of ∼4 kb separated by 574 and 95 bp-long unique sequences.

Mentions: Our previous studies (46,51) revealed that C. metapsilosis, C. orthopsilosis and C. parapsilosis have the same gene order in their mtDNAs. Likewise, C. frijolesensis, C. neerlandica and C. viswanathii have the same genetic organization, except that trnM1 has been duplicated and inverted in the latter species (Figure 5A). All other species examined exhibit unique gene arrangements, with synteny reduced to four conserved gene clusters (i.e. trnN-atp6, cox1-atp9, cob-nad3 and rnl-cox2) in C. jiufengensis versus C. parapsilosis (Figure 5B).Figure 5.


Evolution of linear chromosomes and multipartite genomes in yeast mitochondria.

Valach M, Farkas Z, Fricova D, Kovac J, Brejova B, Vinar T, Pfeiffer I, Kucsera J, Tomaska L, Lang BF, Nosek J - Nucleic Acids Res. (2011)

Comparison of mitochondrial gene orders among species from C. neerlandica–C. tropicalis (A), L. elongisporus–C. parapsilosis (B) and C. subhashii–C. alai (C) lineages. Individual genes and blocks with conserved gene order are shown by identical colors. Duplicated regions are framed. The symbols wedge and caret indicate the orientation of genes, TEL (telomeres) and LIR. In L. elongisporus, both LIRs (LIR*) consist of two regions of ∼4 kb separated by 574 and 95 bp-long unique sequences.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3105423&req=5

Figure 5: Comparison of mitochondrial gene orders among species from C. neerlandica–C. tropicalis (A), L. elongisporus–C. parapsilosis (B) and C. subhashii–C. alai (C) lineages. Individual genes and blocks with conserved gene order are shown by identical colors. Duplicated regions are framed. The symbols wedge and caret indicate the orientation of genes, TEL (telomeres) and LIR. In L. elongisporus, both LIRs (LIR*) consist of two regions of ∼4 kb separated by 574 and 95 bp-long unique sequences.
Mentions: Our previous studies (46,51) revealed that C. metapsilosis, C. orthopsilosis and C. parapsilosis have the same gene order in their mtDNAs. Likewise, C. frijolesensis, C. neerlandica and C. viswanathii have the same genetic organization, except that trnM1 has been duplicated and inverted in the latter species (Figure 5A). All other species examined exhibit unique gene arrangements, with synteny reduced to four conserved gene clusters (i.e. trnN-atp6, cox1-atp9, cob-nad3 and rnl-cox2) in C. jiufengensis versus C. parapsilosis (Figure 5B).Figure 5.

Bottom Line: Our survey revealed a puzzling variability of genome architecture, including circular- and linear-mapping and multipartite linear forms.We propose that the arrangement of large inverted repeats identified in these genomes plays a crucial role in alterations of their molecular architectures.We suggest that molecular transactions generating linear mitochondrial DNA molecules with defined telomeric structures may parallel the evolutionary emergence of linear chromosomes and multipartite genomes in general and may provide clues for the origin of telomeres and pathways implicated in their maintenance.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Comenius University, Mlynska dolina CH-1, 842 15 Bratislava, Slovak republic.

ABSTRACT
Mitochondrial genome diversity in closely related species provides an excellent platform for investigation of chromosome architecture and its evolution by means of comparative genomics. In this study, we determined the complete mitochondrial DNA sequences of eight Candida species and analyzed their molecular architectures. Our survey revealed a puzzling variability of genome architecture, including circular- and linear-mapping and multipartite linear forms. We propose that the arrangement of large inverted repeats identified in these genomes plays a crucial role in alterations of their molecular architectures. In specific arrangements, the inverted repeats appear to function as resolution elements, allowing genome conversion among different topologies, eventually leading to genome fragmentation into multiple linear DNA molecules. We suggest that molecular transactions generating linear mitochondrial DNA molecules with defined telomeric structures may parallel the evolutionary emergence of linear chromosomes and multipartite genomes in general and may provide clues for the origin of telomeres and pathways implicated in their maintenance.

Show MeSH
Related in: MedlinePlus