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Local, persistent activation of Rho GTPases during plasticity of single dendritic spines.

Murakoshi H, Wang H, Yasuda R - Nature (2011)

Bottom Line: Inhibition of the Rho-Rock pathway preferentially inhibited the initial spine growth, whereas the inhibition of the Cdc42-Pak pathway blocked the maintenance of sustained structural plasticity.RhoA and Cdc42 activation depended on Ca(2+)/calmodulin-dependent kinase (CaMKII).Thus, RhoA and Cdc42 relay transient CaMKII activation to synapse-specific, long-term signalling required for spine structural plasticity.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA.

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Spatiotemporal dynamics of Cdc42 activation during long-term structural plasticity induced in single spinesThe same experiments and analyses as in Fig. 1 except for measuring Cdc42 activity instead of RhoA activity. The numbers of samples (spines/neurons) are 33/28 for stimulated spines and dendrite, 33/28 for adjacent spines, 11/6 for KN62 and 12/8 for AP5.
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Figure 2: Spatiotemporal dynamics of Cdc42 activation during long-term structural plasticity induced in single spinesThe same experiments and analyses as in Fig. 1 except for measuring Cdc42 activity instead of RhoA activity. The numbers of samples (spines/neurons) are 33/28 for stimulated spines and dendrite, 33/28 for adjacent spines, 11/6 for KN62 and 12/8 for AP5.

Mentions: Using these sensors, we measured the activity of RhoA and Cdc42 during spine structural plasticity associated with LTP (Figs. 1, 2 and 3). Pyramidal neurons in the CA1 region of cultured hippocampal slices were ballistically18 transfected with the RhoA or Cdc42 sensor, and the FRET signal was imaged under 2pFLIM. The spine volume was monitored using the red fluorescence of mCherry-RBD-mCherry (Supplementary Fig. 3)12. To induce structural plasticity in a single dendritic spine, we applied a low frequency train of two-photon glutamate uncaging pulses (30 pulses at 0.5 Hz) to the spine in zero extracellular Mg2+ (Ref 13,14,19). The spine volume increased rapidly by ~300% following glutamate uncaging (transient phase) and relaxed to an elevated level of 70–80% for more than 30 min (sustained phase) (Figs. 1d, 2d)12–14. The time course of spine enlargement in neurons expressing the FRET sensor was similar to that in neurons expressing only EGFP (Fig. 4)14, suggesting that the overexpression of FRET sensors causes almost no effects on spine structural plasticity (Supplementary note).


Local, persistent activation of Rho GTPases during plasticity of single dendritic spines.

Murakoshi H, Wang H, Yasuda R - Nature (2011)

Spatiotemporal dynamics of Cdc42 activation during long-term structural plasticity induced in single spinesThe same experiments and analyses as in Fig. 1 except for measuring Cdc42 activity instead of RhoA activity. The numbers of samples (spines/neurons) are 33/28 for stimulated spines and dendrite, 33/28 for adjacent spines, 11/6 for KN62 and 12/8 for AP5.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3105377&req=5

Figure 2: Spatiotemporal dynamics of Cdc42 activation during long-term structural plasticity induced in single spinesThe same experiments and analyses as in Fig. 1 except for measuring Cdc42 activity instead of RhoA activity. The numbers of samples (spines/neurons) are 33/28 for stimulated spines and dendrite, 33/28 for adjacent spines, 11/6 for KN62 and 12/8 for AP5.
Mentions: Using these sensors, we measured the activity of RhoA and Cdc42 during spine structural plasticity associated with LTP (Figs. 1, 2 and 3). Pyramidal neurons in the CA1 region of cultured hippocampal slices were ballistically18 transfected with the RhoA or Cdc42 sensor, and the FRET signal was imaged under 2pFLIM. The spine volume was monitored using the red fluorescence of mCherry-RBD-mCherry (Supplementary Fig. 3)12. To induce structural plasticity in a single dendritic spine, we applied a low frequency train of two-photon glutamate uncaging pulses (30 pulses at 0.5 Hz) to the spine in zero extracellular Mg2+ (Ref 13,14,19). The spine volume increased rapidly by ~300% following glutamate uncaging (transient phase) and relaxed to an elevated level of 70–80% for more than 30 min (sustained phase) (Figs. 1d, 2d)12–14. The time course of spine enlargement in neurons expressing the FRET sensor was similar to that in neurons expressing only EGFP (Fig. 4)14, suggesting that the overexpression of FRET sensors causes almost no effects on spine structural plasticity (Supplementary note).

Bottom Line: Inhibition of the Rho-Rock pathway preferentially inhibited the initial spine growth, whereas the inhibition of the Cdc42-Pak pathway blocked the maintenance of sustained structural plasticity.RhoA and Cdc42 activation depended on Ca(2+)/calmodulin-dependent kinase (CaMKII).Thus, RhoA and Cdc42 relay transient CaMKII activation to synapse-specific, long-term signalling required for spine structural plasticity.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA.

Show MeSH
Related in: MedlinePlus