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Comprehensive catecholaminergic projectome analysis reveals single-neuron integration of zebrafish ascending and descending dopaminergic systems.

Tay TL, Ronneberger O, Ryu S, Nitschke R, Driever W - Nat Commun (2011)

Bottom Line: The most extensive DA projections are established by posterior tubercular otp-dependent neurons, with individual somata integrating the ascending DA system, the descending diencephalospinal, as well as the endohypothalamic circuitry.We further identified an endogenous subpallial DA system that not only provides most of the local DA projections, but also connects to the ventral diencephalon.The catecholaminergic projectome map provides a framework to understand the evolution and function of these neuromodulatory systems.

View Article: PubMed Central - PubMed

Affiliation: Developmental Biology, Institute Biology I, Faculty of Biology, Albert-Ludwigs-University Freiburg, Hauptstrasse 1, Freiburg D-79104, Germany.

ABSTRACT
Essential components of animal behaviour are modulated by dopaminergic (DA) and noradrenergic circuitry. In this study, we reveal at cellular resolution the complete set of projections ('projectome') of every single type of DA and noradrenergio neurons in the central nervous system of zebrafish larvae. The most extensive DA projections are established by posterior tubercular otp-dependent neurons, with individual somata integrating the ascending DA system, the descending diencephalospinal, as well as the endohypothalamic circuitry. These findings suggest a major role in the modulation of physiology and behaviour for otp-dependent DA neurons, which correlate with the mammalian A11 group. We further identified an endogenous subpallial DA system that not only provides most of the local DA projections, but also connects to the ventral diencephalon. The catecholaminergic projectome map provides a framework to understand the evolution and function of these neuromodulatory systems.

No MeSH data available.


Related in: MedlinePlus

Target areas of catecholaminergic projections.The target areas (columns) of projections of each catecholaminergic (CA) neuronal group (rows) are indicated quantitatively by the colour code representing the percentage of total neurons per analysed group that project into the area. The total number of neurons analysed is shown in the furthest right column. For CA groups in which individual somata extend projections to multiple target areas, the percentages add up to more than 100. Lavender indicates the neuronal group is liquor contacting. Projections pass through the commissures with the exception of a few cases in which some processes were noted to terminate there: anterior commissure as target of locus coeruleus and area postrema noradrenergic neurons; postoptic commissure as target of anterior preoptic, preoptic, DC1, 2, 4 and 5 dopaminergic neurons. 'L' (for local projection) denotes the termination of neuronal processes within the region in which the soma is located. DC1 includes populations located in the ventral thalamus and periventricular posterior tuberculum.
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f2: Target areas of catecholaminergic projections.The target areas (columns) of projections of each catecholaminergic (CA) neuronal group (rows) are indicated quantitatively by the colour code representing the percentage of total neurons per analysed group that project into the area. The total number of neurons analysed is shown in the furthest right column. For CA groups in which individual somata extend projections to multiple target areas, the percentages add up to more than 100. Lavender indicates the neuronal group is liquor contacting. Projections pass through the commissures with the exception of a few cases in which some processes were noted to terminate there: anterior commissure as target of locus coeruleus and area postrema noradrenergic neurons; postoptic commissure as target of anterior preoptic, preoptic, DC1, 2, 4 and 5 dopaminergic neurons. 'L' (for local projection) denotes the termination of neuronal processes within the region in which the soma is located. DC1 includes populations located in the ventral thalamus and periventricular posterior tuberculum.

Mentions: We established an image analysis workflow to obtain a data set of single CA neuron projections and to identify and classify their projection behaviour (Fig. 1; Supplementary Methods, Supplementary Movie 1 and Supplementary Fig. S2). This imaging technique did not allow us to distinguish between axons and dendrites. Target area anatomical identities were confirmed from histological stains. To comprehensively analyse the CA projection network, we first dissected the characteristics of projections emanating from neurons of each CA group to include directionality (based on the body axes) and type (for example, arbourizing, branching, midline crossing, local; Supplementary Fig. S2). For further classification, major projections that are proximal to the cell soma and target the contralateral side were indicated as contralateral, whereas distal branches that originate from major ipsilateral projections but target contralaterally were documented as midline crossing processes. The second step was to visually trace all CA projections to their anatomical target areas (Fig. 2). Statistical analysis indicates that our data set is large enough to identify most (>80%) of the projection behaviours and target areas.


Comprehensive catecholaminergic projectome analysis reveals single-neuron integration of zebrafish ascending and descending dopaminergic systems.

Tay TL, Ronneberger O, Ryu S, Nitschke R, Driever W - Nat Commun (2011)

Target areas of catecholaminergic projections.The target areas (columns) of projections of each catecholaminergic (CA) neuronal group (rows) are indicated quantitatively by the colour code representing the percentage of total neurons per analysed group that project into the area. The total number of neurons analysed is shown in the furthest right column. For CA groups in which individual somata extend projections to multiple target areas, the percentages add up to more than 100. Lavender indicates the neuronal group is liquor contacting. Projections pass through the commissures with the exception of a few cases in which some processes were noted to terminate there: anterior commissure as target of locus coeruleus and area postrema noradrenergic neurons; postoptic commissure as target of anterior preoptic, preoptic, DC1, 2, 4 and 5 dopaminergic neurons. 'L' (for local projection) denotes the termination of neuronal processes within the region in which the soma is located. DC1 includes populations located in the ventral thalamus and periventricular posterior tuberculum.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3105308&req=5

f2: Target areas of catecholaminergic projections.The target areas (columns) of projections of each catecholaminergic (CA) neuronal group (rows) are indicated quantitatively by the colour code representing the percentage of total neurons per analysed group that project into the area. The total number of neurons analysed is shown in the furthest right column. For CA groups in which individual somata extend projections to multiple target areas, the percentages add up to more than 100. Lavender indicates the neuronal group is liquor contacting. Projections pass through the commissures with the exception of a few cases in which some processes were noted to terminate there: anterior commissure as target of locus coeruleus and area postrema noradrenergic neurons; postoptic commissure as target of anterior preoptic, preoptic, DC1, 2, 4 and 5 dopaminergic neurons. 'L' (for local projection) denotes the termination of neuronal processes within the region in which the soma is located. DC1 includes populations located in the ventral thalamus and periventricular posterior tuberculum.
Mentions: We established an image analysis workflow to obtain a data set of single CA neuron projections and to identify and classify their projection behaviour (Fig. 1; Supplementary Methods, Supplementary Movie 1 and Supplementary Fig. S2). This imaging technique did not allow us to distinguish between axons and dendrites. Target area anatomical identities were confirmed from histological stains. To comprehensively analyse the CA projection network, we first dissected the characteristics of projections emanating from neurons of each CA group to include directionality (based on the body axes) and type (for example, arbourizing, branching, midline crossing, local; Supplementary Fig. S2). For further classification, major projections that are proximal to the cell soma and target the contralateral side were indicated as contralateral, whereas distal branches that originate from major ipsilateral projections but target contralaterally were documented as midline crossing processes. The second step was to visually trace all CA projections to their anatomical target areas (Fig. 2). Statistical analysis indicates that our data set is large enough to identify most (>80%) of the projection behaviours and target areas.

Bottom Line: The most extensive DA projections are established by posterior tubercular otp-dependent neurons, with individual somata integrating the ascending DA system, the descending diencephalospinal, as well as the endohypothalamic circuitry.We further identified an endogenous subpallial DA system that not only provides most of the local DA projections, but also connects to the ventral diencephalon.The catecholaminergic projectome map provides a framework to understand the evolution and function of these neuromodulatory systems.

View Article: PubMed Central - PubMed

Affiliation: Developmental Biology, Institute Biology I, Faculty of Biology, Albert-Ludwigs-University Freiburg, Hauptstrasse 1, Freiburg D-79104, Germany.

ABSTRACT
Essential components of animal behaviour are modulated by dopaminergic (DA) and noradrenergic circuitry. In this study, we reveal at cellular resolution the complete set of projections ('projectome') of every single type of DA and noradrenergio neurons in the central nervous system of zebrafish larvae. The most extensive DA projections are established by posterior tubercular otp-dependent neurons, with individual somata integrating the ascending DA system, the descending diencephalospinal, as well as the endohypothalamic circuitry. These findings suggest a major role in the modulation of physiology and behaviour for otp-dependent DA neurons, which correlate with the mammalian A11 group. We further identified an endogenous subpallial DA system that not only provides most of the local DA projections, but also connects to the ventral diencephalon. The catecholaminergic projectome map provides a framework to understand the evolution and function of these neuromodulatory systems.

No MeSH data available.


Related in: MedlinePlus