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Ancient origins determine global biogeography of hot and cold desert cyanobacteria.

Bahl J, Lau MC, Smith GJ, Vijaykrishna D, Cary SC, Lacap DC, Lee CK, Papke RT, Warren-Rhodes KA, Wong FK, McKay CP, Pointing SB - Nat Commun (2011)

Bottom Line: Multilocus phylogenetic associations were dependent on contemporary climate with no evidence for distance-related patterns.These results indicate that global distribution of desert cyanobacteria has not resulted from widespread contemporary dispersal but is an ancient evolutionary legacy.This highlights the importance of considering temporal scales in microbial biogeography.

View Article: PubMed Central - PubMed

Affiliation: Duke-NUS Graduate Medical School, Singapore 169857.

ABSTRACT
Factors governing large-scale spatio-temporal distribution of microorganisms remain unresolved, yet are pivotal to understanding ecosystem value and function. Molecular genetic analyses have focused on the influence of niche and neutral processes in determining spatial patterns without considering the temporal scale. Here, we use temporal phylogenetic analysis calibrated using microfossil data for a globally sampled desert cyanobacterium, Chroococcidiopsis, to investigate spatio-temporal patterns in microbial biogeography and evolution. Multilocus phylogenetic associations were dependent on contemporary climate with no evidence for distance-related patterns. Massively parallel pyrosequencing of environmental samples confirmed that Chroococcidiopsis variants were specific to either hot or cold deserts. Temporally scaled phylogenetic analyses showed no evidence of recent inter-regional gene flow, indicating populations have not shared common ancestry since before the formation of modern continents. These results indicate that global distribution of desert cyanobacteria has not resulted from widespread contemporary dispersal but is an ancient evolutionary legacy. This highlights the importance of considering temporal scales in microbial biogeography.

No MeSH data available.


Related in: MedlinePlus

Global distribution of Chroococcidiopsis variants.The map indicates hot (brown boxes) and cold (blue boxes) desert locations from which variants were recovered. Detailed site descriptions and climatic information are given in Supplementary Table S1.
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f1: Global distribution of Chroococcidiopsis variants.The map indicates hot (brown boxes) and cold (blue boxes) desert locations from which variants were recovered. Detailed site descriptions and climatic information are given in Supplementary Table S1.

Mentions: We assembled an extensive sequence-based data set for the ubiquitously distributed desert cyanobacterial genus Chroococcidiopsis from every major desert on the Earth (Fig. 1). These habitats included both hot and cold desert climates. We successfully recovered environmental DNA from hypolithic cyanobacterial biomass on translucent quartz. Clone libraries generated from each environmental sample were screened for Chroococcidiopsis phylotypes by analysis of 16S rRNA gene sequences and their 16S rRNA, 5.8S ITS and 23S rRNA loci were then sequenced for phylogenetic analysis. A total of 73 unique phylotypes were identified and sequenced (Supplementary Table S1).


Ancient origins determine global biogeography of hot and cold desert cyanobacteria.

Bahl J, Lau MC, Smith GJ, Vijaykrishna D, Cary SC, Lacap DC, Lee CK, Papke RT, Warren-Rhodes KA, Wong FK, McKay CP, Pointing SB - Nat Commun (2011)

Global distribution of Chroococcidiopsis variants.The map indicates hot (brown boxes) and cold (blue boxes) desert locations from which variants were recovered. Detailed site descriptions and climatic information are given in Supplementary Table S1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3105302&req=5

f1: Global distribution of Chroococcidiopsis variants.The map indicates hot (brown boxes) and cold (blue boxes) desert locations from which variants were recovered. Detailed site descriptions and climatic information are given in Supplementary Table S1.
Mentions: We assembled an extensive sequence-based data set for the ubiquitously distributed desert cyanobacterial genus Chroococcidiopsis from every major desert on the Earth (Fig. 1). These habitats included both hot and cold desert climates. We successfully recovered environmental DNA from hypolithic cyanobacterial biomass on translucent quartz. Clone libraries generated from each environmental sample were screened for Chroococcidiopsis phylotypes by analysis of 16S rRNA gene sequences and their 16S rRNA, 5.8S ITS and 23S rRNA loci were then sequenced for phylogenetic analysis. A total of 73 unique phylotypes were identified and sequenced (Supplementary Table S1).

Bottom Line: Multilocus phylogenetic associations were dependent on contemporary climate with no evidence for distance-related patterns.These results indicate that global distribution of desert cyanobacteria has not resulted from widespread contemporary dispersal but is an ancient evolutionary legacy.This highlights the importance of considering temporal scales in microbial biogeography.

View Article: PubMed Central - PubMed

Affiliation: Duke-NUS Graduate Medical School, Singapore 169857.

ABSTRACT
Factors governing large-scale spatio-temporal distribution of microorganisms remain unresolved, yet are pivotal to understanding ecosystem value and function. Molecular genetic analyses have focused on the influence of niche and neutral processes in determining spatial patterns without considering the temporal scale. Here, we use temporal phylogenetic analysis calibrated using microfossil data for a globally sampled desert cyanobacterium, Chroococcidiopsis, to investigate spatio-temporal patterns in microbial biogeography and evolution. Multilocus phylogenetic associations were dependent on contemporary climate with no evidence for distance-related patterns. Massively parallel pyrosequencing of environmental samples confirmed that Chroococcidiopsis variants were specific to either hot or cold deserts. Temporally scaled phylogenetic analyses showed no evidence of recent inter-regional gene flow, indicating populations have not shared common ancestry since before the formation of modern continents. These results indicate that global distribution of desert cyanobacteria has not resulted from widespread contemporary dispersal but is an ancient evolutionary legacy. This highlights the importance of considering temporal scales in microbial biogeography.

No MeSH data available.


Related in: MedlinePlus