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FOXP3, IL-10, and TGF-β genes expression in children with IgE-dependent food allergy.

Krogulska A, Borowiec M, Polakowska E, Dynowski J, Młynarski W, Wasowska-Królikowska K - J. Clin. Immunol. (2010)

Bottom Line: The significant tendency toward lower total IgE levels with a higher FOXP3 mRNA expression was detected (n = 54; Pearson r = -0.4393; p = 0.001).Children with FA showed statistically significant lower level of the FOXP3 and IL10 gene expression than healthy children.The correlation between the level of FOXP3 and total IgE was detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Allergology, Gastroenterology and Nutrition, Medical University of Lodz, Sporna 36/50, 91-738, Lodz, Poland. anetkrog@poczta.onet.pl

ABSTRACT

Background: Regulatory T cells (Tregs) have an essential role in tolerance and immune regulation. However, few and controversial data have been published to date on the role and number of these cells in food allergic children. The forkhead/winged-helix transcription factor box protein 3 (FOXP3) is considered the most reliable marker for Tregs.

Objective: This study aims to investigate the FOXP3, interleukin (IL)-10, and transforming growth factor (TGF-β) genes expression in children with IgE-dependent food allergy.

Material and methods: The study group consisted of 54 children with IgE-dependent food allergy (FA) and a control group of 26 non-atopic healthy children. The diagnosis of FA was established using questionnaires, clinical criteria, skin prick tests, serum sIgE antibodies (UniCAP 100 Pharmacia Upjohn), and a double-blind placebo control food challenge. In order to assess gene expression, the isolation of nucleated cells was performed using Histopaque-1077 (Sigma-Aldrich, Germany). The concentration of RNA obtained was measured using a super-sensitive NanoDrop ND1000 spectrophotometer (Thermo Scientific, USA). A reverse transcription reaction was performed using a commercially available set of High Capacity cDNA Archive Kit (Applied Biosystems, USA). Analysis have been carried out in the genetic analyzer 7900HT Real-Time PCR (Applied Biosystems, USA).

Results: The average level of the FOXP3 gene expression in the studied group was 2.19 ± 1.16 and in the control group 2.88 ± 1.66 (p = 0.03). The average level of IL10 mRNA expression in the study group was 13.6 ± 1.07 and was significantly lower than corresponding values in the control group 14.3 ± 1.1 (p = 0.01). There were no significant differences in the average level of the TGF-β mRNA expression in the study group (3.4 ± 0.4) and controls (3.5 ± 0.3; p > 0.05). The FOXP3 gene expression was the highest in children who acquired tolerance to food (3.54 ± 0.75), lower in heated allergen-tolerant children (2.43 ± 0.81), and the lowest in heated allergen-reactive children (1.18 ± 0.5; p = 0.001 control vs heated allergen reactive; p = 0.005 heated allergen tolerant vs heated allergen reactive; p = 0.001 outgrown vs heated allergen reactive). The significant tendency toward lower total IgE levels with a higher FOXP3 mRNA expression was detected (n = 54; Pearson r = -0.4393; p = 0.001).

Conclusions: Children with FA showed statistically significant lower level of the FOXP3 and IL10 gene expression than healthy children. Children acquiring tolerance to the food show significantly higher levels of the FOXP3 gene expression than children with active FA. The correlation between the level of FOXP3 and total IgE was detected.

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a. FOXP3 expression in correlation with tIgE in study group. b. FOXP3 expression in correlation with tIgE and reaction grade during food challenge
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Fig3: a. FOXP3 expression in correlation with tIgE in study group. b. FOXP3 expression in correlation with tIgE and reaction grade during food challenge

Mentions: There was no statistically significant correlation between FOXP3, IL-10, or TGF-β expression and age (r = −0.0605; r = 0.2478; r = −0.1651; p > 0.05), although there was a significant negative correlation between the average level of expression of IL-10 and age in children up to 3 years of life, which was not found in older children (r = −0.3876; p = 0.04). The significant tendency toward lower total IgE (tIgE) levels with higher FOXP3 mRNA expression was detected (r = −0.4393; p = 0.001; Fig. 3a). There was no correlation between IL-10 or TGF-β expression and tIgE levels (r = 0.0185; r = 0.0193; p > 0.05). Likewise, there was no statistically significant correlation between FOXP3, IL-10, or TGF-β expression and sIgE level (r = −0.0285; r = 0.0449; r = 0.0873; p > 0.05).Fig. 3


FOXP3, IL-10, and TGF-β genes expression in children with IgE-dependent food allergy.

Krogulska A, Borowiec M, Polakowska E, Dynowski J, Młynarski W, Wasowska-Królikowska K - J. Clin. Immunol. (2010)

a. FOXP3 expression in correlation with tIgE in study group. b. FOXP3 expression in correlation with tIgE and reaction grade during food challenge
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3105233&req=5

Fig3: a. FOXP3 expression in correlation with tIgE in study group. b. FOXP3 expression in correlation with tIgE and reaction grade during food challenge
Mentions: There was no statistically significant correlation between FOXP3, IL-10, or TGF-β expression and age (r = −0.0605; r = 0.2478; r = −0.1651; p > 0.05), although there was a significant negative correlation between the average level of expression of IL-10 and age in children up to 3 years of life, which was not found in older children (r = −0.3876; p = 0.04). The significant tendency toward lower total IgE (tIgE) levels with higher FOXP3 mRNA expression was detected (r = −0.4393; p = 0.001; Fig. 3a). There was no correlation between IL-10 or TGF-β expression and tIgE levels (r = 0.0185; r = 0.0193; p > 0.05). Likewise, there was no statistically significant correlation between FOXP3, IL-10, or TGF-β expression and sIgE level (r = −0.0285; r = 0.0449; r = 0.0873; p > 0.05).Fig. 3

Bottom Line: The significant tendency toward lower total IgE levels with a higher FOXP3 mRNA expression was detected (n = 54; Pearson r = -0.4393; p = 0.001).Children with FA showed statistically significant lower level of the FOXP3 and IL10 gene expression than healthy children.The correlation between the level of FOXP3 and total IgE was detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Allergology, Gastroenterology and Nutrition, Medical University of Lodz, Sporna 36/50, 91-738, Lodz, Poland. anetkrog@poczta.onet.pl

ABSTRACT

Background: Regulatory T cells (Tregs) have an essential role in tolerance and immune regulation. However, few and controversial data have been published to date on the role and number of these cells in food allergic children. The forkhead/winged-helix transcription factor box protein 3 (FOXP3) is considered the most reliable marker for Tregs.

Objective: This study aims to investigate the FOXP3, interleukin (IL)-10, and transforming growth factor (TGF-β) genes expression in children with IgE-dependent food allergy.

Material and methods: The study group consisted of 54 children with IgE-dependent food allergy (FA) and a control group of 26 non-atopic healthy children. The diagnosis of FA was established using questionnaires, clinical criteria, skin prick tests, serum sIgE antibodies (UniCAP 100 Pharmacia Upjohn), and a double-blind placebo control food challenge. In order to assess gene expression, the isolation of nucleated cells was performed using Histopaque-1077 (Sigma-Aldrich, Germany). The concentration of RNA obtained was measured using a super-sensitive NanoDrop ND1000 spectrophotometer (Thermo Scientific, USA). A reverse transcription reaction was performed using a commercially available set of High Capacity cDNA Archive Kit (Applied Biosystems, USA). Analysis have been carried out in the genetic analyzer 7900HT Real-Time PCR (Applied Biosystems, USA).

Results: The average level of the FOXP3 gene expression in the studied group was 2.19 ± 1.16 and in the control group 2.88 ± 1.66 (p = 0.03). The average level of IL10 mRNA expression in the study group was 13.6 ± 1.07 and was significantly lower than corresponding values in the control group 14.3 ± 1.1 (p = 0.01). There were no significant differences in the average level of the TGF-β mRNA expression in the study group (3.4 ± 0.4) and controls (3.5 ± 0.3; p > 0.05). The FOXP3 gene expression was the highest in children who acquired tolerance to food (3.54 ± 0.75), lower in heated allergen-tolerant children (2.43 ± 0.81), and the lowest in heated allergen-reactive children (1.18 ± 0.5; p = 0.001 control vs heated allergen reactive; p = 0.005 heated allergen tolerant vs heated allergen reactive; p = 0.001 outgrown vs heated allergen reactive). The significant tendency toward lower total IgE levels with a higher FOXP3 mRNA expression was detected (n = 54; Pearson r = -0.4393; p = 0.001).

Conclusions: Children with FA showed statistically significant lower level of the FOXP3 and IL10 gene expression than healthy children. Children acquiring tolerance to the food show significantly higher levels of the FOXP3 gene expression than children with active FA. The correlation between the level of FOXP3 and total IgE was detected.

Show MeSH
Related in: MedlinePlus