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Comparative metaproteomic analysis on consecutively Rehmannia glutinosa-monocultured rhizosphere soil.

Wu L, Wang H, Zhang Z, Lin R, Zhang Z, Lin W - PLoS ONE (2011)

Bottom Line: The consecutive monoculture for most of medicinal plants, such as Rehmannia glutinosa, results in a significant reduction in the yield and quality.The results suggest that the consecutive monoculture of R. glutinosa changes the soil microbial ecology due to the exudates accumulation, as a result, the nutrient cycles are affected, leading to the retardation of plant growth and development.Our results demonstrated the interactions among plant, soil and microflora in the proteomic level are crucial for the productivity and quality of R. glutinosa in consecutive monoculture system.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

ABSTRACT

Background: The consecutive monoculture for most of medicinal plants, such as Rehmannia glutinosa, results in a significant reduction in the yield and quality. There is an urgent need to study for the sustainable development of Chinese herbaceous medicine.

Methodology/principal findings: Comparative metaproteomics of rhizosphere soil was developed and used to analyze the underlying mechanism of the consecutive monoculture problems of R. glutinosa. The 2D-gel patterns of protein spots for the soil samples showed a strong matrix dependency. Among the spots, 103 spots with high resolution and repeatability were randomly selected and successfully identified by MALDI TOF-TOF MS for a rhizosphere soil metaproteomic profile analysis. These proteins originating from plants and microorganisms play important roles in nutrient cycles and energy flow in rhizospheric soil ecosystem. They function in protein, nucleotide and secondary metabolisms, signal transduction and resistance. Comparative metaproteomics analysis revealed 33 differentially expressed protein spots in rhizosphere soil in response to increasing years of monoculture. Among them, plant proteins related to carbon and nitrogen metabolism and stress response, were mostly up-regulated except a down-regulated protein (glutathione S-transferase) involving detoxification. The phenylalanine ammonia-lyase was believed to participate in the phenylpropanoid metabolism as shown with a considerable increase in total phenolic acid content with increasing years of monoculture. Microbial proteins related to protein metabolism and cell wall biosynthesis, were up-regulated except a down-regulated protein (geranylgeranyl pyrophosphate synthase) functioning in diterpenoid synthesis. The results suggest that the consecutive monoculture of R. glutinosa changes the soil microbial ecology due to the exudates accumulation, as a result, the nutrient cycles are affected, leading to the retardation of plant growth and development.

Conclusions/significance: Our results demonstrated the interactions among plant, soil and microflora in the proteomic level are crucial for the productivity and quality of R. glutinosa in consecutive monoculture system.

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Related in: MedlinePlus

Expression levels of 33 identified proteins as compared to control.Changes in protein expression under consecutive monoculture were calculated by Image Master software 5.0.
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pone-0020611-g005: Expression levels of 33 identified proteins as compared to control.Changes in protein expression under consecutive monoculture were calculated by Image Master software 5.0.

Mentions: Since the soil proteins have a significant effect on the soil biological process, a comparative analysis was carried out to analyze the changes of metaproteomic characterization in the consecutively R. glutinosa-monocultured rhizosphere soil. A quantitative analysis revealed that a total of 33 protein spots with high repeatability were differentially expressed, i.e., their intensities varied, at least on one gel in comparison to the control, by more than 1.5-fold. Among the differentially expressed proteins, 4 spots (spots 14, 18, 20 and 145, constituting 12.12%) were down-regulated with the increasing years of monoculture. Nine spots (spots 6, 9, 10, 16, 33, 50, 53, 131 and 135, constituting 27.27%) were up-regulated only in the two-year monoculture soil, but none in the one-year monoculture soil. One spot (spot 23, constituting 3.03%) was up-regulated only in the one-year monoculture soil, but none in the two-year monoculture soil. Two spots (spots 21 and 60, constituting 6.06%) were down-regulated only in the two-year monoculture soil, but none in the one-year monoculture soil. The remainders (spots 1, 2, 3, 4, 5, 7, 8, 11, 12, 13, 15, 17, 19, 39, 45, 46 and 111, constituting 51.52%) were all up-regulated with the increasing years of monoculture (Figure 5).


Comparative metaproteomic analysis on consecutively Rehmannia glutinosa-monocultured rhizosphere soil.

Wu L, Wang H, Zhang Z, Lin R, Zhang Z, Lin W - PLoS ONE (2011)

Expression levels of 33 identified proteins as compared to control.Changes in protein expression under consecutive monoculture were calculated by Image Master software 5.0.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3105091&req=5

pone-0020611-g005: Expression levels of 33 identified proteins as compared to control.Changes in protein expression under consecutive monoculture were calculated by Image Master software 5.0.
Mentions: Since the soil proteins have a significant effect on the soil biological process, a comparative analysis was carried out to analyze the changes of metaproteomic characterization in the consecutively R. glutinosa-monocultured rhizosphere soil. A quantitative analysis revealed that a total of 33 protein spots with high repeatability were differentially expressed, i.e., their intensities varied, at least on one gel in comparison to the control, by more than 1.5-fold. Among the differentially expressed proteins, 4 spots (spots 14, 18, 20 and 145, constituting 12.12%) were down-regulated with the increasing years of monoculture. Nine spots (spots 6, 9, 10, 16, 33, 50, 53, 131 and 135, constituting 27.27%) were up-regulated only in the two-year monoculture soil, but none in the one-year monoculture soil. One spot (spot 23, constituting 3.03%) was up-regulated only in the one-year monoculture soil, but none in the two-year monoculture soil. Two spots (spots 21 and 60, constituting 6.06%) were down-regulated only in the two-year monoculture soil, but none in the one-year monoculture soil. The remainders (spots 1, 2, 3, 4, 5, 7, 8, 11, 12, 13, 15, 17, 19, 39, 45, 46 and 111, constituting 51.52%) were all up-regulated with the increasing years of monoculture (Figure 5).

Bottom Line: The consecutive monoculture for most of medicinal plants, such as Rehmannia glutinosa, results in a significant reduction in the yield and quality.The results suggest that the consecutive monoculture of R. glutinosa changes the soil microbial ecology due to the exudates accumulation, as a result, the nutrient cycles are affected, leading to the retardation of plant growth and development.Our results demonstrated the interactions among plant, soil and microflora in the proteomic level are crucial for the productivity and quality of R. glutinosa in consecutive monoculture system.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

ABSTRACT

Background: The consecutive monoculture for most of medicinal plants, such as Rehmannia glutinosa, results in a significant reduction in the yield and quality. There is an urgent need to study for the sustainable development of Chinese herbaceous medicine.

Methodology/principal findings: Comparative metaproteomics of rhizosphere soil was developed and used to analyze the underlying mechanism of the consecutive monoculture problems of R. glutinosa. The 2D-gel patterns of protein spots for the soil samples showed a strong matrix dependency. Among the spots, 103 spots with high resolution and repeatability were randomly selected and successfully identified by MALDI TOF-TOF MS for a rhizosphere soil metaproteomic profile analysis. These proteins originating from plants and microorganisms play important roles in nutrient cycles and energy flow in rhizospheric soil ecosystem. They function in protein, nucleotide and secondary metabolisms, signal transduction and resistance. Comparative metaproteomics analysis revealed 33 differentially expressed protein spots in rhizosphere soil in response to increasing years of monoculture. Among them, plant proteins related to carbon and nitrogen metabolism and stress response, were mostly up-regulated except a down-regulated protein (glutathione S-transferase) involving detoxification. The phenylalanine ammonia-lyase was believed to participate in the phenylpropanoid metabolism as shown with a considerable increase in total phenolic acid content with increasing years of monoculture. Microbial proteins related to protein metabolism and cell wall biosynthesis, were up-regulated except a down-regulated protein (geranylgeranyl pyrophosphate synthase) functioning in diterpenoid synthesis. The results suggest that the consecutive monoculture of R. glutinosa changes the soil microbial ecology due to the exudates accumulation, as a result, the nutrient cycles are affected, leading to the retardation of plant growth and development.

Conclusions/significance: Our results demonstrated the interactions among plant, soil and microflora in the proteomic level are crucial for the productivity and quality of R. glutinosa in consecutive monoculture system.

Show MeSH
Related in: MedlinePlus