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Comparative metaproteomic analysis on consecutively Rehmannia glutinosa-monocultured rhizosphere soil.

Wu L, Wang H, Zhang Z, Lin R, Zhang Z, Lin W - PLoS ONE (2011)

Bottom Line: The consecutive monoculture for most of medicinal plants, such as Rehmannia glutinosa, results in a significant reduction in the yield and quality.The results suggest that the consecutive monoculture of R. glutinosa changes the soil microbial ecology due to the exudates accumulation, as a result, the nutrient cycles are affected, leading to the retardation of plant growth and development.Our results demonstrated the interactions among plant, soil and microflora in the proteomic level are crucial for the productivity and quality of R. glutinosa in consecutive monoculture system.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

ABSTRACT

Background: The consecutive monoculture for most of medicinal plants, such as Rehmannia glutinosa, results in a significant reduction in the yield and quality. There is an urgent need to study for the sustainable development of Chinese herbaceous medicine.

Methodology/principal findings: Comparative metaproteomics of rhizosphere soil was developed and used to analyze the underlying mechanism of the consecutive monoculture problems of R. glutinosa. The 2D-gel patterns of protein spots for the soil samples showed a strong matrix dependency. Among the spots, 103 spots with high resolution and repeatability were randomly selected and successfully identified by MALDI TOF-TOF MS for a rhizosphere soil metaproteomic profile analysis. These proteins originating from plants and microorganisms play important roles in nutrient cycles and energy flow in rhizospheric soil ecosystem. They function in protein, nucleotide and secondary metabolisms, signal transduction and resistance. Comparative metaproteomics analysis revealed 33 differentially expressed protein spots in rhizosphere soil in response to increasing years of monoculture. Among them, plant proteins related to carbon and nitrogen metabolism and stress response, were mostly up-regulated except a down-regulated protein (glutathione S-transferase) involving detoxification. The phenylalanine ammonia-lyase was believed to participate in the phenylpropanoid metabolism as shown with a considerable increase in total phenolic acid content with increasing years of monoculture. Microbial proteins related to protein metabolism and cell wall biosynthesis, were up-regulated except a down-regulated protein (geranylgeranyl pyrophosphate synthase) functioning in diterpenoid synthesis. The results suggest that the consecutive monoculture of R. glutinosa changes the soil microbial ecology due to the exudates accumulation, as a result, the nutrient cycles are affected, leading to the retardation of plant growth and development.

Conclusions/significance: Our results demonstrated the interactions among plant, soil and microflora in the proteomic level are crucial for the productivity and quality of R. glutinosa in consecutive monoculture system.

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Related in: MedlinePlus

Silver stained 2-D gel of proteins extracted from rhizosphere soil.A: Proteins extracted from the control soil. B: Proteins extracted from the one-year R. glutinosa-monocultured soil; C: Proteins extracted from the two-year R. glutinosa-monocultured soil. D: Repeatability analysis of 2-DE maps of soil proteins extracted from three different soil samples. Arrows in A point at proteins with differential expressions. Upward arrows in B and C indicate the positions of up-regulated proteins and downward arrows show the positions of down-regulated proteins, while white circles in B and C represent the same expression level compared to the control. Scatter plots in D give an idea of the relationship between the spot values (%Vol) from two gels (CK vs NP, CK vs CM) by searching for the linear dependence between the spot values of one gel (variable X, namely NP or CM) and the corresponding values in a reference gel (variable Y, namely CK).
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pone-0020611-g001: Silver stained 2-D gel of proteins extracted from rhizosphere soil.A: Proteins extracted from the control soil. B: Proteins extracted from the one-year R. glutinosa-monocultured soil; C: Proteins extracted from the two-year R. glutinosa-monocultured soil. D: Repeatability analysis of 2-DE maps of soil proteins extracted from three different soil samples. Arrows in A point at proteins with differential expressions. Upward arrows in B and C indicate the positions of up-regulated proteins and downward arrows show the positions of down-regulated proteins, while white circles in B and C represent the same expression level compared to the control. Scatter plots in D give an idea of the relationship between the spot values (%Vol) from two gels (CK vs NP, CK vs CM) by searching for the linear dependence between the spot values of one gel (variable X, namely NP or CM) and the corresponding values in a reference gel (variable Y, namely CK).

Mentions: Further analysis was done to investigate the changes of the proteins from rhizosphere soil samples in response to the consecutive monoculture. A high resolution 2-DE gel protein separation was applied in the pI range between 5 and 8. After silver staining, protein spots were isolated and analyzed using the ImageMasterâ„¢ 2D Platinum software (version 5.0, GE Healthcare, Uppsala, Sweden). Highly reproducible 2-DE maps were obtained in the three different soil samples with significant correlations of scatter plots (Figure 1). The correlation index between the control soils and the one-year monoculture soils, and the correlation index between the control soils and the two-year monoculture soils were 0.772 and 0.812, respectively. All 2-DE images had a similar spot distribution pattern, implying that they shared similar bio-information.


Comparative metaproteomic analysis on consecutively Rehmannia glutinosa-monocultured rhizosphere soil.

Wu L, Wang H, Zhang Z, Lin R, Zhang Z, Lin W - PLoS ONE (2011)

Silver stained 2-D gel of proteins extracted from rhizosphere soil.A: Proteins extracted from the control soil. B: Proteins extracted from the one-year R. glutinosa-monocultured soil; C: Proteins extracted from the two-year R. glutinosa-monocultured soil. D: Repeatability analysis of 2-DE maps of soil proteins extracted from three different soil samples. Arrows in A point at proteins with differential expressions. Upward arrows in B and C indicate the positions of up-regulated proteins and downward arrows show the positions of down-regulated proteins, while white circles in B and C represent the same expression level compared to the control. Scatter plots in D give an idea of the relationship between the spot values (%Vol) from two gels (CK vs NP, CK vs CM) by searching for the linear dependence between the spot values of one gel (variable X, namely NP or CM) and the corresponding values in a reference gel (variable Y, namely CK).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3105091&req=5

pone-0020611-g001: Silver stained 2-D gel of proteins extracted from rhizosphere soil.A: Proteins extracted from the control soil. B: Proteins extracted from the one-year R. glutinosa-monocultured soil; C: Proteins extracted from the two-year R. glutinosa-monocultured soil. D: Repeatability analysis of 2-DE maps of soil proteins extracted from three different soil samples. Arrows in A point at proteins with differential expressions. Upward arrows in B and C indicate the positions of up-regulated proteins and downward arrows show the positions of down-regulated proteins, while white circles in B and C represent the same expression level compared to the control. Scatter plots in D give an idea of the relationship between the spot values (%Vol) from two gels (CK vs NP, CK vs CM) by searching for the linear dependence between the spot values of one gel (variable X, namely NP or CM) and the corresponding values in a reference gel (variable Y, namely CK).
Mentions: Further analysis was done to investigate the changes of the proteins from rhizosphere soil samples in response to the consecutive monoculture. A high resolution 2-DE gel protein separation was applied in the pI range between 5 and 8. After silver staining, protein spots were isolated and analyzed using the ImageMasterâ„¢ 2D Platinum software (version 5.0, GE Healthcare, Uppsala, Sweden). Highly reproducible 2-DE maps were obtained in the three different soil samples with significant correlations of scatter plots (Figure 1). The correlation index between the control soils and the one-year monoculture soils, and the correlation index between the control soils and the two-year monoculture soils were 0.772 and 0.812, respectively. All 2-DE images had a similar spot distribution pattern, implying that they shared similar bio-information.

Bottom Line: The consecutive monoculture for most of medicinal plants, such as Rehmannia glutinosa, results in a significant reduction in the yield and quality.The results suggest that the consecutive monoculture of R. glutinosa changes the soil microbial ecology due to the exudates accumulation, as a result, the nutrient cycles are affected, leading to the retardation of plant growth and development.Our results demonstrated the interactions among plant, soil and microflora in the proteomic level are crucial for the productivity and quality of R. glutinosa in consecutive monoculture system.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

ABSTRACT

Background: The consecutive monoculture for most of medicinal plants, such as Rehmannia glutinosa, results in a significant reduction in the yield and quality. There is an urgent need to study for the sustainable development of Chinese herbaceous medicine.

Methodology/principal findings: Comparative metaproteomics of rhizosphere soil was developed and used to analyze the underlying mechanism of the consecutive monoculture problems of R. glutinosa. The 2D-gel patterns of protein spots for the soil samples showed a strong matrix dependency. Among the spots, 103 spots with high resolution and repeatability were randomly selected and successfully identified by MALDI TOF-TOF MS for a rhizosphere soil metaproteomic profile analysis. These proteins originating from plants and microorganisms play important roles in nutrient cycles and energy flow in rhizospheric soil ecosystem. They function in protein, nucleotide and secondary metabolisms, signal transduction and resistance. Comparative metaproteomics analysis revealed 33 differentially expressed protein spots in rhizosphere soil in response to increasing years of monoculture. Among them, plant proteins related to carbon and nitrogen metabolism and stress response, were mostly up-regulated except a down-regulated protein (glutathione S-transferase) involving detoxification. The phenylalanine ammonia-lyase was believed to participate in the phenylpropanoid metabolism as shown with a considerable increase in total phenolic acid content with increasing years of monoculture. Microbial proteins related to protein metabolism and cell wall biosynthesis, were up-regulated except a down-regulated protein (geranylgeranyl pyrophosphate synthase) functioning in diterpenoid synthesis. The results suggest that the consecutive monoculture of R. glutinosa changes the soil microbial ecology due to the exudates accumulation, as a result, the nutrient cycles are affected, leading to the retardation of plant growth and development.

Conclusions/significance: Our results demonstrated the interactions among plant, soil and microflora in the proteomic level are crucial for the productivity and quality of R. glutinosa in consecutive monoculture system.

Show MeSH
Related in: MedlinePlus