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The production of extracellular proteins is regulated by ribonuclease III via two different pathways in Staphylococcus aureus.

Liu Y, Dong J, Wu N, Gao Y, Zhang X, Mu C, Shao N, Fan M, Yang G - PLoS ONE (2011)

Bottom Line: It was found that the extracellular proteins of Δrnc were decreased.We found during the lag phase of the bacterial growth cycle RNase III could influence the extracellular protein secretion via regulating the expression of secY2, one component of accessory secretory (sec) pathway.Our results suggest that RNase III could regulate the pathogenicity of S. aureus by influencing the level of extracellular proteins via two different ways respectively at different growth phases.

View Article: PubMed Central - PubMed

Affiliation: Beijing Institute of Basic Medical Sciences, Beijing, People's Republic of China.

ABSTRACT
Staphylococcus aureus ribonuclease III belongs to the enzyme family known to degrade double-stranded RNAs. It has previously been reported that RNase III cannot influence cell growth but regulates virulence gene expression in S. aureus. Here we constructed an RNase III inactivation mutant (Δrnc) from S. aureus 8325-4. It was found that the extracellular proteins of Δrnc were decreased. Furthermore, we explored how RNase III regulated the production of the extracellular proteins in S. aureus. We found during the lag phase of the bacterial growth cycle RNase III could influence the extracellular protein secretion via regulating the expression of secY2, one component of accessory secretory (sec) pathway. After S. aureus cells grew to exponential phase, RNase III can regulate the expression of extracellular proteins by affecting the level of RNAIII. Further investigation showed that the mRNA stability of secY2 and RNAIII was affected by RNase III. Our results suggest that RNase III could regulate the pathogenicity of S. aureus by influencing the level of extracellular proteins via two different ways respectively at different growth phases.

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Stability of RNAs.Half-lives of secY2 mRNA and RNAIII were determined in the presence of rifampicin (500 µg ml-1) in the WT and Δrnc strains. Percentage of RNA was calculated normalizing with 5s rRNA.
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pone-0020554-g006: Stability of RNAs.Half-lives of secY2 mRNA and RNAIII were determined in the presence of rifampicin (500 µg ml-1) in the WT and Δrnc strains. Percentage of RNA was calculated normalizing with 5s rRNA.

Mentions: In order to investigate how RNase III influences the expression level of secY2 and RNAIII, we tested the RNA stability of secY2 mRNA and RNAIII. The transcriptions of secY2 and RNAIII were inhibited by addition of rifampicin when the cells had been cultured at 37°C for 1.5 h or 6 h individually. Then the RNA stability was tested by Northern blot. It was found that RNA stability of secY2 mRNA and RNAIII was decreased in Δrnc compared with its parent strain (figure 6).


The production of extracellular proteins is regulated by ribonuclease III via two different pathways in Staphylococcus aureus.

Liu Y, Dong J, Wu N, Gao Y, Zhang X, Mu C, Shao N, Fan M, Yang G - PLoS ONE (2011)

Stability of RNAs.Half-lives of secY2 mRNA and RNAIII were determined in the presence of rifampicin (500 µg ml-1) in the WT and Δrnc strains. Percentage of RNA was calculated normalizing with 5s rRNA.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3105085&req=5

pone-0020554-g006: Stability of RNAs.Half-lives of secY2 mRNA and RNAIII were determined in the presence of rifampicin (500 µg ml-1) in the WT and Δrnc strains. Percentage of RNA was calculated normalizing with 5s rRNA.
Mentions: In order to investigate how RNase III influences the expression level of secY2 and RNAIII, we tested the RNA stability of secY2 mRNA and RNAIII. The transcriptions of secY2 and RNAIII were inhibited by addition of rifampicin when the cells had been cultured at 37°C for 1.5 h or 6 h individually. Then the RNA stability was tested by Northern blot. It was found that RNA stability of secY2 mRNA and RNAIII was decreased in Δrnc compared with its parent strain (figure 6).

Bottom Line: It was found that the extracellular proteins of Δrnc were decreased.We found during the lag phase of the bacterial growth cycle RNase III could influence the extracellular protein secretion via regulating the expression of secY2, one component of accessory secretory (sec) pathway.Our results suggest that RNase III could regulate the pathogenicity of S. aureus by influencing the level of extracellular proteins via two different ways respectively at different growth phases.

View Article: PubMed Central - PubMed

Affiliation: Beijing Institute of Basic Medical Sciences, Beijing, People's Republic of China.

ABSTRACT
Staphylococcus aureus ribonuclease III belongs to the enzyme family known to degrade double-stranded RNAs. It has previously been reported that RNase III cannot influence cell growth but regulates virulence gene expression in S. aureus. Here we constructed an RNase III inactivation mutant (Δrnc) from S. aureus 8325-4. It was found that the extracellular proteins of Δrnc were decreased. Furthermore, we explored how RNase III regulated the production of the extracellular proteins in S. aureus. We found during the lag phase of the bacterial growth cycle RNase III could influence the extracellular protein secretion via regulating the expression of secY2, one component of accessory secretory (sec) pathway. After S. aureus cells grew to exponential phase, RNase III can regulate the expression of extracellular proteins by affecting the level of RNAIII. Further investigation showed that the mRNA stability of secY2 and RNAIII was affected by RNase III. Our results suggest that RNase III could regulate the pathogenicity of S. aureus by influencing the level of extracellular proteins via two different ways respectively at different growth phases.

Show MeSH
Related in: MedlinePlus