Limits...
Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy.

Zheng M, Lv LL, Ni J, Ni HF, Li Q, Ma KL, Liu BC - PLoS ONE (2011)

Bottom Line: Quantification of messenger RNA (mRNA) expression in urinary sediment by real-time PCR is emerging as a noninvasive method of screening DN-associated biomarkers.Furthermore, podocalyxin mRNA was found to negatively correlate with eGFR (r = -0.349, p = 0.01).The urinary mRNA profiles of synaptopodin, podocalyxin, CD2-AP, α-actin4, and podocin were found to increase with the progression of DN, which suggested that quantification of podocyte-associated molecules will be useful biomarkers of DN.

View Article: PubMed Central - PubMed

Affiliation: Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing, China.

ABSTRACT

Background: Podocyte injury and subsequent excretion in urine play a crucial role in the pathogenesis and progression of diabetic nephropathy (DN). Quantification of messenger RNA (mRNA) expression in urinary sediment by real-time PCR is emerging as a noninvasive method of screening DN-associated biomarkers. We hypothesized that the urinary mRNA profile of podocyte-associated molecules may provide important clinical insight into the different stages of diabetic nephropathy.

Methods: DN patients (N = 51) and healthy controls (N = 13) were enrolled in this study. DN patients were divided into a normoalbuminuria group (UAE<30 mg/g, n = 17), a microalbuminuria group (UAE 30∼300 mg/g, n = 15), and a macroalbuminuria group (UAE>300 mg/g, n = 19), according to their urinary albumin excretion (UAE). Relative mRNA abundance of synaptopodin, podocalyxin, CD2-AP, α-actin4, and podocin were quantified, and correlations between target mRNAs and clinical parameters were examined.

Results: The urinary mRNA levels of all genes studied were significantly higher in the DN group compared with controls (p<0.05), and mRNA levels increased with DN progression. Urinary mRNA levels of all target genes positively correlated with both UAE and BUN. The expression of podocalyxin, CD2-AP, α-actin4, and podocin mRNA correlated with serum creatinine (r = 0.457, p = 0.001; r = 0.329, p = 0.01; r = 0.286, p = 0.021; r = 0.357, p = 0.006, respectively). Furthermore, podocalyxin mRNA was found to negatively correlate with eGFR (r = -0.349, p = 0.01).

Conclusion: The urinary mRNA profiles of synaptopodin, podocalyxin, CD2-AP, α-actin4, and podocin were found to increase with the progression of DN, which suggested that quantification of podocyte-associated molecules will be useful biomarkers of DN.

Show MeSH

Related in: MedlinePlus

Relationships between mRNA expression of podocyte-associated molecules in urinary sediment and baseline parameters.albuminuria (A), serum creatinine (B) and BUN (C). Data were compared using the Spearman correlation coefficient.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3105067&req=5

pone-0020431-g003: Relationships between mRNA expression of podocyte-associated molecules in urinary sediment and baseline parameters.albuminuria (A), serum creatinine (B) and BUN (C). Data were compared using the Spearman correlation coefficient.

Mentions: No significant correlations between urinary mRNA expression of podocyte markers and age were found (r = 0.133, p = 0.289 for α-actin4, r = 0.018, p = 0.894 for podocin, r = −0.007, p = 0.955 for CD2-AP, r = 0.15, p = 0.279 for podocalyxin, and r = 0.12, p = 0.351 for synaptopodin using the Spearman rank-order correlation). The correlations between these markers and clinical parameters of renal function, such as urinary albumin, BUN, serum creatinine and eGFR, are summarized in figure 3. In general, the urinary mRNA levels of all target molecules were significantly correlated with urinary albumin (r = 0.478, p<0.001 for α-actin4, r = 0.378, p = 0.003 for podocin, r = 0.402, p = 0.001 for CD2-AP, r = 0.457, p<0.001 for podocalyxin, and r = 0.384, p = 0.001 for synaptopodin using the Spearman rank-order correlation). Also, a significant positive correlation was observed between BUN and urinary α-actin4 mRNA (r = 0.353, p = 0.004), CD2-AP mRNA (r = 0.303, p = 0.018), podocalyxin mRNA (r = 0.474, p<0.001) and synaptopodin mRNA levels (r = 0.359, p = 0.004). Moreover, the expression of podocalyxin, CD2-AP, α-actin4, and podocin mRNA correlated with serum creatinine levels (r = 0.457, p = 0.001; r = 0.329, p = 0.01; r = 0.286, p = 0.021; r = 0.357, p = 0.006, respectively). However, eGFR levels did not significantly relate to the mRNA levels of synaptopodin (r = −0.146, p = 0.255), CD2-AP (r = −0.209, p = 0.106), α-actin4 (r = −0.227, p = 0.069), or podocin(r = −0.202, p = 0.127), but did show a significant correlation with podocalyxin expression (r = −0.349, p = 0.01).


Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy.

Zheng M, Lv LL, Ni J, Ni HF, Li Q, Ma KL, Liu BC - PLoS ONE (2011)

Relationships between mRNA expression of podocyte-associated molecules in urinary sediment and baseline parameters.albuminuria (A), serum creatinine (B) and BUN (C). Data were compared using the Spearman correlation coefficient.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3105067&req=5

pone-0020431-g003: Relationships between mRNA expression of podocyte-associated molecules in urinary sediment and baseline parameters.albuminuria (A), serum creatinine (B) and BUN (C). Data were compared using the Spearman correlation coefficient.
Mentions: No significant correlations between urinary mRNA expression of podocyte markers and age were found (r = 0.133, p = 0.289 for α-actin4, r = 0.018, p = 0.894 for podocin, r = −0.007, p = 0.955 for CD2-AP, r = 0.15, p = 0.279 for podocalyxin, and r = 0.12, p = 0.351 for synaptopodin using the Spearman rank-order correlation). The correlations between these markers and clinical parameters of renal function, such as urinary albumin, BUN, serum creatinine and eGFR, are summarized in figure 3. In general, the urinary mRNA levels of all target molecules were significantly correlated with urinary albumin (r = 0.478, p<0.001 for α-actin4, r = 0.378, p = 0.003 for podocin, r = 0.402, p = 0.001 for CD2-AP, r = 0.457, p<0.001 for podocalyxin, and r = 0.384, p = 0.001 for synaptopodin using the Spearman rank-order correlation). Also, a significant positive correlation was observed between BUN and urinary α-actin4 mRNA (r = 0.353, p = 0.004), CD2-AP mRNA (r = 0.303, p = 0.018), podocalyxin mRNA (r = 0.474, p<0.001) and synaptopodin mRNA levels (r = 0.359, p = 0.004). Moreover, the expression of podocalyxin, CD2-AP, α-actin4, and podocin mRNA correlated with serum creatinine levels (r = 0.457, p = 0.001; r = 0.329, p = 0.01; r = 0.286, p = 0.021; r = 0.357, p = 0.006, respectively). However, eGFR levels did not significantly relate to the mRNA levels of synaptopodin (r = −0.146, p = 0.255), CD2-AP (r = −0.209, p = 0.106), α-actin4 (r = −0.227, p = 0.069), or podocin(r = −0.202, p = 0.127), but did show a significant correlation with podocalyxin expression (r = −0.349, p = 0.01).

Bottom Line: Quantification of messenger RNA (mRNA) expression in urinary sediment by real-time PCR is emerging as a noninvasive method of screening DN-associated biomarkers.Furthermore, podocalyxin mRNA was found to negatively correlate with eGFR (r = -0.349, p = 0.01).The urinary mRNA profiles of synaptopodin, podocalyxin, CD2-AP, α-actin4, and podocin were found to increase with the progression of DN, which suggested that quantification of podocyte-associated molecules will be useful biomarkers of DN.

View Article: PubMed Central - PubMed

Affiliation: Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing, China.

ABSTRACT

Background: Podocyte injury and subsequent excretion in urine play a crucial role in the pathogenesis and progression of diabetic nephropathy (DN). Quantification of messenger RNA (mRNA) expression in urinary sediment by real-time PCR is emerging as a noninvasive method of screening DN-associated biomarkers. We hypothesized that the urinary mRNA profile of podocyte-associated molecules may provide important clinical insight into the different stages of diabetic nephropathy.

Methods: DN patients (N = 51) and healthy controls (N = 13) were enrolled in this study. DN patients were divided into a normoalbuminuria group (UAE<30 mg/g, n = 17), a microalbuminuria group (UAE 30∼300 mg/g, n = 15), and a macroalbuminuria group (UAE>300 mg/g, n = 19), according to their urinary albumin excretion (UAE). Relative mRNA abundance of synaptopodin, podocalyxin, CD2-AP, α-actin4, and podocin were quantified, and correlations between target mRNAs and clinical parameters were examined.

Results: The urinary mRNA levels of all genes studied were significantly higher in the DN group compared with controls (p<0.05), and mRNA levels increased with DN progression. Urinary mRNA levels of all target genes positively correlated with both UAE and BUN. The expression of podocalyxin, CD2-AP, α-actin4, and podocin mRNA correlated with serum creatinine (r = 0.457, p = 0.001; r = 0.329, p = 0.01; r = 0.286, p = 0.021; r = 0.357, p = 0.006, respectively). Furthermore, podocalyxin mRNA was found to negatively correlate with eGFR (r = -0.349, p = 0.01).

Conclusion: The urinary mRNA profiles of synaptopodin, podocalyxin, CD2-AP, α-actin4, and podocin were found to increase with the progression of DN, which suggested that quantification of podocyte-associated molecules will be useful biomarkers of DN.

Show MeSH
Related in: MedlinePlus