Limits...
Colocalization of 14-3-3 proteins with SOD1 in Lewy body-like hyaline inclusions in familial amyotrophic lateral sclerosis cases and the animal model.

Okamoto Y, Shirakashi Y, Ihara M, Urushitani M, Oono M, Kawamoto Y, Yamashita H, Shimohama S, Kato S, Hirano A, Tomimoto H, Ito H, Takahashi R - PLoS ONE (2011)

Bottom Line: Cu/Zn superoxide dismutase (SOD1) is a major component of Lewy body-like hyaline inclusion (LBHI) found in the postmortem tissue of SOD1-linked familial amyotrophic lateral sclerosis (FALS) patients.In our recent studies, 14-3-3 proteins have been found in the ubiquitinated inclusions inside the anterior horn cells of spinal cords with sporadic amyotrophic lateral sclerosis (ALS).We examined the postmortem brains and the spinal cords of three FALS cases (A4V SOD1 mutant).

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Kyoto University Graduate School of Medicine, Kyoto, Japan.

ABSTRACT

Background and purpose: Cu/Zn superoxide dismutase (SOD1) is a major component of Lewy body-like hyaline inclusion (LBHI) found in the postmortem tissue of SOD1-linked familial amyotrophic lateral sclerosis (FALS) patients. In our recent studies, 14-3-3 proteins have been found in the ubiquitinated inclusions inside the anterior horn cells of spinal cords with sporadic amyotrophic lateral sclerosis (ALS). To further investigate the role of 14-3-3 proteins in ALS, we performed immunohistochemical analysis of 14-3-3 proteins and compared their distributions with those of SOD1 in FALS patients and SOD1-overexpressing mice.

Methods: We examined the postmortem brains and the spinal cords of three FALS cases (A4V SOD1 mutant). Transgenic mice expressing the G93A mutant human SOD1 (mutant SOD1-Tg mice), transgenic mice expressing the wild-type human SOD1 (wild-type SOD1-Tg mice), and non-Tg wild-type mice were also subjected to the immunohistochemical analysis.

Results: In all the FALS patients, LBHIs were observed in the cytoplasm of the anterior horn cells, and these inclusions were immunopositive intensely for pan 14-3-3, 14-3-3β, and 14-3-3γ. In the mutant SOD1-Tg mice, a high degree of immunoreactivity for misfolded SOD1 (C4F6) was observed in the cytoplasm, with an even greater degree of immunoreactivity present in the cytoplasmic aggregates of the anterior horn cells in the lumbar spinal cord. Furthermore, we have found increased 14-3-3β and 14-3-3γ immunoreactivities in the mutant SOD1-Tg mice. Double immunofluorescent staining showed that C4F6 and 14-3-3 proteins were partially co-localized in the spinal cord with FALS and the mutant SOD1-Tg mice. In comparison, the wild-type SOD1-Tg and non-Tg wild-type mice showed no or faint immunoreactivity for C4F6 and 14-3-3 proteins (pan 14-3-3, 14-3-3β, and 14-3-3γ) in any neuronal compartments.

Discussion: These results suggest that 14-3-3 proteins may be associated with the formation of SOD1-containing inclusions, in FALS patients and the mutant SOD1-Tg mice.

Show MeSH

Related in: MedlinePlus

Neuronal inclusions immunopositive for C4F6 in mice.Strong immunoreactivity for C4F6 (A) was observed in the somatodendritic compartment with cytoplasmic inclusions in the mutant SOD1-Tg mice. Immunoreactivity for C4F6 was restricted to glial cells morphologically consistent with microglia in the wild-type SOD1-Tg mice (B) and absent in the non-Tg wild-type mice (C). Bar indicates 50 µm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3105059&req=5

pone-0020427-g004: Neuronal inclusions immunopositive for C4F6 in mice.Strong immunoreactivity for C4F6 (A) was observed in the somatodendritic compartment with cytoplasmic inclusions in the mutant SOD1-Tg mice. Immunoreactivity for C4F6 was restricted to glial cells morphologically consistent with microglia in the wild-type SOD1-Tg mice (B) and absent in the non-Tg wild-type mice (C). Bar indicates 50 µm.

Mentions: In mutant SOD1-Tg mice, C4F6 immunoreactivity was observed in the remaining anterior horn cells with cytoplasmic inclusions (Figure 4A). Immunoreactivity for C4F6 was restricted to the glial cells that were morphologically consistent with microglia in wild-type SOD1-Tg mice (Figure 4B) and absent in non-Tg wild-type mice (Figure 4C).


Colocalization of 14-3-3 proteins with SOD1 in Lewy body-like hyaline inclusions in familial amyotrophic lateral sclerosis cases and the animal model.

Okamoto Y, Shirakashi Y, Ihara M, Urushitani M, Oono M, Kawamoto Y, Yamashita H, Shimohama S, Kato S, Hirano A, Tomimoto H, Ito H, Takahashi R - PLoS ONE (2011)

Neuronal inclusions immunopositive for C4F6 in mice.Strong immunoreactivity for C4F6 (A) was observed in the somatodendritic compartment with cytoplasmic inclusions in the mutant SOD1-Tg mice. Immunoreactivity for C4F6 was restricted to glial cells morphologically consistent with microglia in the wild-type SOD1-Tg mice (B) and absent in the non-Tg wild-type mice (C). Bar indicates 50 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3105059&req=5

pone-0020427-g004: Neuronal inclusions immunopositive for C4F6 in mice.Strong immunoreactivity for C4F6 (A) was observed in the somatodendritic compartment with cytoplasmic inclusions in the mutant SOD1-Tg mice. Immunoreactivity for C4F6 was restricted to glial cells morphologically consistent with microglia in the wild-type SOD1-Tg mice (B) and absent in the non-Tg wild-type mice (C). Bar indicates 50 µm.
Mentions: In mutant SOD1-Tg mice, C4F6 immunoreactivity was observed in the remaining anterior horn cells with cytoplasmic inclusions (Figure 4A). Immunoreactivity for C4F6 was restricted to the glial cells that were morphologically consistent with microglia in wild-type SOD1-Tg mice (Figure 4B) and absent in non-Tg wild-type mice (Figure 4C).

Bottom Line: Cu/Zn superoxide dismutase (SOD1) is a major component of Lewy body-like hyaline inclusion (LBHI) found in the postmortem tissue of SOD1-linked familial amyotrophic lateral sclerosis (FALS) patients.In our recent studies, 14-3-3 proteins have been found in the ubiquitinated inclusions inside the anterior horn cells of spinal cords with sporadic amyotrophic lateral sclerosis (ALS).We examined the postmortem brains and the spinal cords of three FALS cases (A4V SOD1 mutant).

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Kyoto University Graduate School of Medicine, Kyoto, Japan.

ABSTRACT

Background and purpose: Cu/Zn superoxide dismutase (SOD1) is a major component of Lewy body-like hyaline inclusion (LBHI) found in the postmortem tissue of SOD1-linked familial amyotrophic lateral sclerosis (FALS) patients. In our recent studies, 14-3-3 proteins have been found in the ubiquitinated inclusions inside the anterior horn cells of spinal cords with sporadic amyotrophic lateral sclerosis (ALS). To further investigate the role of 14-3-3 proteins in ALS, we performed immunohistochemical analysis of 14-3-3 proteins and compared their distributions with those of SOD1 in FALS patients and SOD1-overexpressing mice.

Methods: We examined the postmortem brains and the spinal cords of three FALS cases (A4V SOD1 mutant). Transgenic mice expressing the G93A mutant human SOD1 (mutant SOD1-Tg mice), transgenic mice expressing the wild-type human SOD1 (wild-type SOD1-Tg mice), and non-Tg wild-type mice were also subjected to the immunohistochemical analysis.

Results: In all the FALS patients, LBHIs were observed in the cytoplasm of the anterior horn cells, and these inclusions were immunopositive intensely for pan 14-3-3, 14-3-3β, and 14-3-3γ. In the mutant SOD1-Tg mice, a high degree of immunoreactivity for misfolded SOD1 (C4F6) was observed in the cytoplasm, with an even greater degree of immunoreactivity present in the cytoplasmic aggregates of the anterior horn cells in the lumbar spinal cord. Furthermore, we have found increased 14-3-3β and 14-3-3γ immunoreactivities in the mutant SOD1-Tg mice. Double immunofluorescent staining showed that C4F6 and 14-3-3 proteins were partially co-localized in the spinal cord with FALS and the mutant SOD1-Tg mice. In comparison, the wild-type SOD1-Tg and non-Tg wild-type mice showed no or faint immunoreactivity for C4F6 and 14-3-3 proteins (pan 14-3-3, 14-3-3β, and 14-3-3γ) in any neuronal compartments.

Discussion: These results suggest that 14-3-3 proteins may be associated with the formation of SOD1-containing inclusions, in FALS patients and the mutant SOD1-Tg mice.

Show MeSH
Related in: MedlinePlus